Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor
Abstract Arhopalus unicolor is a carrier of the pine wood nematode (PWN), which causes pine wilt disease, killing pine trees and causing considerable economic and environmental losses. While the A. unicolor mitochondrial genome has been published, a high-quality genome assembly and annotation of A....
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Nature Portfolio
2025-01-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04460-8 |
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| author | Xiangying Zou Liqiong Zeng Xingzao You Jinghui Zhong Chensheng Tang Zhaofei Zheng Xiaochun Lin Biaoqiang Zhang Xinliang Zhang Yu Fang Yanli Sun Shouping Cai Jun Su |
| author_facet | Xiangying Zou Liqiong Zeng Xingzao You Jinghui Zhong Chensheng Tang Zhaofei Zheng Xiaochun Lin Biaoqiang Zhang Xinliang Zhang Yu Fang Yanli Sun Shouping Cai Jun Su |
| author_sort | Xiangying Zou |
| collection | DOAJ |
| description | Abstract Arhopalus unicolor is a carrier of the pine wood nematode (PWN), which causes pine wilt disease, killing pine trees and causing considerable economic and environmental losses. While the A. unicolor mitochondrial genome has been published, a high-quality genome assembly and annotation of A. unicolor is not yet available. To address this, we assembled a chromosome-level reference genome assembly of A. unicolor with a combination of Illumina, PacBio, and Hi-C sequencing technologies. The final genome size was determined to be 1268.11 Mb, with a GC% of 32.44%, and the scaffold N50 value was 19.30 Mb. A total of 98.77% of the assembled sequences mapped to 10 pseudochromosomes, and BUSCO analysis revealed high completeness, with 97.15% gene coverage. Furthermore, the genome contains 71.74% repeat elements and encompasses 16,450 predicted protein-coding genes. This genome sequence of A. unicolor will be a valuable resource for understanding the genetics and evolutionary history of this species and for developing effective management strategies for this PWN carrier. |
| format | Article |
| id | doaj-art-b57fd77c85ee4fcbb4c161fd09027aef |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-b57fd77c85ee4fcbb4c161fd09027aef2025-01-26T12:14:53ZengNature PortfolioScientific Data2052-44632025-01-011211810.1038/s41597-025-04460-8Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolorXiangying Zou0Liqiong Zeng1Xingzao You2Jinghui Zhong3Chensheng Tang4Zhaofei Zheng5Xiaochun Lin6Biaoqiang Zhang7Xinliang Zhang8Yu Fang9Yanli Sun10Shouping Cai11Jun Su12Key Laboratory of State Forestry and Grassland Administration on Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of ForestryKey Laboratory of State Forestry and Grassland Administration on Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of ForestryNanping Forestry Science and Technology Promotion CenterQuanzhou Forestry Pest Control and Quarantine StationKey Laboratory of State Forestry and Grassland Administration on Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of ForestryNanping Forestry BureauFuding Forestry BureauShaxian Guanzhuang State-owned Forest Farm of Fujian ProvinceShaxian Forestry Pest Control and Quarantine StationInstitute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural SciencesLanshan Forestry Development CentreKey Laboratory of State Forestry and Grassland Administration on Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China, Fujian Academy of ForestryFujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Haixia Institute of Science and Technology, Fujian Agriculture and Forestry University, College of Life SciencesAbstract Arhopalus unicolor is a carrier of the pine wood nematode (PWN), which causes pine wilt disease, killing pine trees and causing considerable economic and environmental losses. While the A. unicolor mitochondrial genome has been published, a high-quality genome assembly and annotation of A. unicolor is not yet available. To address this, we assembled a chromosome-level reference genome assembly of A. unicolor with a combination of Illumina, PacBio, and Hi-C sequencing technologies. The final genome size was determined to be 1268.11 Mb, with a GC% of 32.44%, and the scaffold N50 value was 19.30 Mb. A total of 98.77% of the assembled sequences mapped to 10 pseudochromosomes, and BUSCO analysis revealed high completeness, with 97.15% gene coverage. Furthermore, the genome contains 71.74% repeat elements and encompasses 16,450 predicted protein-coding genes. This genome sequence of A. unicolor will be a valuable resource for understanding the genetics and evolutionary history of this species and for developing effective management strategies for this PWN carrier.https://doi.org/10.1038/s41597-025-04460-8 |
| spellingShingle | Xiangying Zou Liqiong Zeng Xingzao You Jinghui Zhong Chensheng Tang Zhaofei Zheng Xiaochun Lin Biaoqiang Zhang Xinliang Zhang Yu Fang Yanli Sun Shouping Cai Jun Su Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor Scientific Data |
| title | Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor |
| title_full | Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor |
| title_fullStr | Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor |
| title_full_unstemmed | Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor |
| title_short | Chromosome-level genome assembly of the pine wood nematode carrier Arhopalus unicolor |
| title_sort | chromosome level genome assembly of the pine wood nematode carrier arhopalus unicolor |
| url | https://doi.org/10.1038/s41597-025-04460-8 |
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