Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry
A new stability-indicating reversed-phase high-performance thin-layer chromatographic (RP-HPTLC) method for densitometric analysis of thiocolchicoside was developed and validated. The chromatograms were developed using aluminum plates pre-coated with silica gel 60 RP-18 F254S as a stationary phase a...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/142628 |
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| author | Dnyansing K. Rajput Atul A. Shirkhedkar Jyoti K. Rajput Harun M. Patel Sanjay J. Surana |
| author_facet | Dnyansing K. Rajput Atul A. Shirkhedkar Jyoti K. Rajput Harun M. Patel Sanjay J. Surana |
| author_sort | Dnyansing K. Rajput |
| collection | DOAJ |
| description | A new stability-indicating reversed-phase high-performance thin-layer chromatographic (RP-HPTLC) method for densitometric analysis of thiocolchicoside was developed and validated. The chromatograms were developed using aluminum plates pre-coated with silica gel 60 RP-18 F254S as a stationary phase and methanol : water (70 : 30 ) as a mobile phase. The compact band for thiocolchicoside was observed at value of at an absorption wavelength of 377 nm. The linear regression data for the calibration plots () was found with respect to peak area in the concentration range of 100–600 ng per band. The limit of detection (LOD) and limit of quantification (LOQ) were 9.77 ng and 29.63 ng, respectively. The drug was exposed to acidic and alkaline hydrolysis, oxidation, photo degradation, and dry heat conditions. The peaks of degradation products were well-resolved from the peak of the standard drug with significantly different values. Statistical analysis proved that the established RP-HPTLC method is reproducible, selective, and accurate for the determination of thiocolchicoside in its formulations. The method can effectively separate the drug from its degradation products, and it can be considered as stability-indicating assay. |
| format | Article |
| id | doaj-art-b5649562dc094f62ac1069808c1b87b0 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-b5649562dc094f62ac1069808c1b87b02025-02-03T06:13:08ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/142628142628Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/DensitometryDnyansing K. Rajput0Atul A. Shirkhedkar1Jyoti K. Rajput2Harun M. Patel3Sanjay J. Surana4Department of Quality Assurance, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule 425 405, IndiaDepartment of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule 425 405, IndiaDepartment of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule 425 405, IndiaDepartment of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule 425 405, IndiaR. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dhule 425 405, IndiaA new stability-indicating reversed-phase high-performance thin-layer chromatographic (RP-HPTLC) method for densitometric analysis of thiocolchicoside was developed and validated. The chromatograms were developed using aluminum plates pre-coated with silica gel 60 RP-18 F254S as a stationary phase and methanol : water (70 : 30 ) as a mobile phase. The compact band for thiocolchicoside was observed at value of at an absorption wavelength of 377 nm. The linear regression data for the calibration plots () was found with respect to peak area in the concentration range of 100–600 ng per band. The limit of detection (LOD) and limit of quantification (LOQ) were 9.77 ng and 29.63 ng, respectively. The drug was exposed to acidic and alkaline hydrolysis, oxidation, photo degradation, and dry heat conditions. The peaks of degradation products were well-resolved from the peak of the standard drug with significantly different values. Statistical analysis proved that the established RP-HPTLC method is reproducible, selective, and accurate for the determination of thiocolchicoside in its formulations. The method can effectively separate the drug from its degradation products, and it can be considered as stability-indicating assay.http://dx.doi.org/10.1155/2013/142628 |
| spellingShingle | Dnyansing K. Rajput Atul A. Shirkhedkar Jyoti K. Rajput Harun M. Patel Sanjay J. Surana Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry Journal of Analytical Methods in Chemistry |
| title | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
| title_full | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
| title_fullStr | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
| title_full_unstemmed | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
| title_short | Stability Studies of Thiocolchicoside in Bulk and Capsules Using RP-HPTLC/Densitometry |
| title_sort | stability studies of thiocolchicoside in bulk and capsules using rp hptlc densitometry |
| url | http://dx.doi.org/10.1155/2013/142628 |
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