Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation
ADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation and differentiation, metabolism, stress, and immunity in plants and animals. The involvement of ADP-ribosylation in the life cycle of...
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2025-01-01
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| author | Viktoriya O. Samarskaya Sofya Koblova Tatiana Suprunova Eugene A. Rogozhin Nadezhda Spechenkova Sofiya Yakunina Andrew J. Love Natalia O. Kalinina Michael Taliansky |
| author_facet | Viktoriya O. Samarskaya Sofya Koblova Tatiana Suprunova Eugene A. Rogozhin Nadezhda Spechenkova Sofiya Yakunina Andrew J. Love Natalia O. Kalinina Michael Taliansky |
| author_sort | Viktoriya O. Samarskaya |
| collection | DOAJ |
| description | ADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation and differentiation, metabolism, stress, and immunity in plants and animals. The involvement of ADP-ribosylation in the life cycle of <i>Dictyostelium</i> and some filamentous fungi has also been demonstrated. However, the role of this process in pathogenic oomycetes has never been addressed. Here, we show that the <i>Phytophthora infestans</i> genome contains two PARP-like protein genes (PiPARP1 and PiPARP2), and provide evidence of PARylation activity for one of them (PiPARP2). Using dsRNA-mediated RNA silencing of the PiPARP2 gene and chemical (pharmacological) inhibition of PARP activity by 3-aminobenzamide (3AB) PARP inhibitor, we demonstrate the critical functional role of ADP-ribosylation in <i>Phytophthora</i> mycelium growth. Virulence test on detached leaves also suggests an important role of ADP-ribosylation in <i>Phytophthora</i> host plant colonisation and pathogenesis. On a practical level, our data suggest that targeting the PARylation system may constitute a novel powerful approach for the management of <i>Phytophthora</i> diseases. |
| format | Article |
| id | doaj-art-b5520d595e254ad5910cec62f4e324dc |
| institution | Kabale University |
| issn | 2309-608X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj-art-b5520d595e254ad5910cec62f4e324dc2025-01-24T13:37:17ZengMDPI AGJournal of Fungi2309-608X2025-01-011112910.3390/jof11010029Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant ColonisationViktoriya O. Samarskaya0Sofya Koblova1Tatiana Suprunova2Eugene A. Rogozhin3Nadezhda Spechenkova4Sofiya Yakunina5Andrew J. Love6Natalia O. Kalinina7Michael Taliansky8Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaDoka-Gene Technologies Ltd., Moscow Region, 141880 Rogachevo, RussiaDoka-Gene Technologies Ltd., Moscow Region, 141880 Rogachevo, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaJames Hutton Institute, Invergowrie, Dundee DD2 5DA, UKBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119991 Moscow, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, 117997 Moscow, RussiaADP-ribosylation is a reversible modification of proteins and nucleic acids, which controls major cellular processes, including DNA damage repair, cell proliferation and differentiation, metabolism, stress, and immunity in plants and animals. The involvement of ADP-ribosylation in the life cycle of <i>Dictyostelium</i> and some filamentous fungi has also been demonstrated. However, the role of this process in pathogenic oomycetes has never been addressed. Here, we show that the <i>Phytophthora infestans</i> genome contains two PARP-like protein genes (PiPARP1 and PiPARP2), and provide evidence of PARylation activity for one of them (PiPARP2). Using dsRNA-mediated RNA silencing of the PiPARP2 gene and chemical (pharmacological) inhibition of PARP activity by 3-aminobenzamide (3AB) PARP inhibitor, we demonstrate the critical functional role of ADP-ribosylation in <i>Phytophthora</i> mycelium growth. Virulence test on detached leaves also suggests an important role of ADP-ribosylation in <i>Phytophthora</i> host plant colonisation and pathogenesis. On a practical level, our data suggest that targeting the PARylation system may constitute a novel powerful approach for the management of <i>Phytophthora</i> diseases.https://www.mdpi.com/2309-608X/11/1/29<i>Phytophthora infestans</i>potatoRNA interferencedsRNApoly ADP-ribosylationPARP inhibitor |
| spellingShingle | Viktoriya O. Samarskaya Sofya Koblova Tatiana Suprunova Eugene A. Rogozhin Nadezhda Spechenkova Sofiya Yakunina Andrew J. Love Natalia O. Kalinina Michael Taliansky Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation Journal of Fungi <i>Phytophthora infestans</i> potato RNA interference dsRNA poly ADP-ribosylation PARP inhibitor |
| title | Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation |
| title_full | Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation |
| title_fullStr | Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation |
| title_full_unstemmed | Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation |
| title_short | Poly ADP-Ribosylation in a Plant Pathogenic Oomycete <i>Phytophthora infestans</i>: A Key Controller of Growth and Host Plant Colonisation |
| title_sort | poly adp ribosylation in a plant pathogenic oomycete i phytophthora infestans i a key controller of growth and host plant colonisation |
| topic | <i>Phytophthora infestans</i> potato RNA interference dsRNA poly ADP-ribosylation PARP inhibitor |
| url | https://www.mdpi.com/2309-608X/11/1/29 |
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