Genetic Uniqueness and Pomological Diversity Among the Apple Accessions Maintained Within the Croatian National Clonal Germplasm Repository
In the Croatian apple germplasm, there are, presumably, unique genotypes that have not yet been documented in reference molecular databases. Due to similarities between accessions, incorrect names are often used, which creates a problem in the identification of accessions. Overall, 169 apple accessi...
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2025-01-01
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| author | Danijel Čiček Almira Konjić Martina Skendrović Babojelić Predrag Vujević Silvio Šimon Fuad Gaši |
| author_facet | Danijel Čiček Almira Konjić Martina Skendrović Babojelić Predrag Vujević Silvio Šimon Fuad Gaši |
| author_sort | Danijel Čiček |
| collection | DOAJ |
| description | In the Croatian apple germplasm, there are, presumably, unique genotypes that have not yet been documented in reference molecular databases. Due to similarities between accessions, incorrect names are often used, which creates a problem in the identification of accessions. Overall, 169 apple accessions and 11 reference cultivars from the largest ex situ apple collection in the Republic of Croatia were investigated within this study. The examined accessions have been genotyped using SSR markers. In order to assess the advantage of a high-resolution marker system, such as SNPs, compared to low-resolution markers, such as SSRs, a subset of 23 unique apple accessions and eight reference cultivars were genotyped using the 480K Affymetrix Axiom SNP array. Results obtained through the use of two marker systems revealed 26 synonyms, 40 duplicates, 13 mislabeling accessions, 45 accessions with confirmed identity (known cultivars), and 45 unique accessions, as well as the true identity of a large number of accessions, currently maintained at the Croatian National Apple Germplasm Bank. In order to investigate the pomological variability of unique apples, a three-year study was carried out on eleven pomological traits. The researched germplasm shows an exceptional diversity of pomological properties. Many of the accessions can be considered unique, and the results of the pomological characterization indicated that this germplasm contains valuable traits of interest for future breeding programs. |
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| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Agronomy |
| spelling | doaj-art-b541cc49f25543d7b3bd4c7e9ff93da52025-01-24T13:16:46ZengMDPI AGAgronomy2073-43952025-01-0115111310.3390/agronomy15010113Genetic Uniqueness and Pomological Diversity Among the Apple Accessions Maintained Within the Croatian National Clonal Germplasm RepositoryDanijel Čiček0Almira Konjić1Martina Skendrović Babojelić2Predrag Vujević3Silvio Šimon4Fuad Gaši5Croatian Agency for Agriculture and Food, Center of Pomology and Vegetable Crops, Gorice 68b, 10000 Zagreb, CroatiaFaculty of Agriculture and Food Sciences, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaFaculty of Agriculture, University of Zagreb, Svetošimunska Cesta 25, 10000 Zagreb, CroatiaCroatian Agency for Agriculture and Food, Center of Pomology and Vegetable Crops, Gorice 68b, 10000 Zagreb, CroatiaMinistry of Agriculture, Forestry and Fisheries, Ulica Grada Vukovara 78, 10000 Zagreb, CroatiaFaculty of Agriculture and Food Sciences, University of Sarajevo, Zmaja od Bosne 8, 71000 Sarajevo, Bosnia and HerzegovinaIn the Croatian apple germplasm, there are, presumably, unique genotypes that have not yet been documented in reference molecular databases. Due to similarities between accessions, incorrect names are often used, which creates a problem in the identification of accessions. Overall, 169 apple accessions and 11 reference cultivars from the largest ex situ apple collection in the Republic of Croatia were investigated within this study. The examined accessions have been genotyped using SSR markers. In order to assess the advantage of a high-resolution marker system, such as SNPs, compared to low-resolution markers, such as SSRs, a subset of 23 unique apple accessions and eight reference cultivars were genotyped using the 480K Affymetrix Axiom SNP array. Results obtained through the use of two marker systems revealed 26 synonyms, 40 duplicates, 13 mislabeling accessions, 45 accessions with confirmed identity (known cultivars), and 45 unique accessions, as well as the true identity of a large number of accessions, currently maintained at the Croatian National Apple Germplasm Bank. In order to investigate the pomological variability of unique apples, a three-year study was carried out on eleven pomological traits. The researched germplasm shows an exceptional diversity of pomological properties. Many of the accessions can be considered unique, and the results of the pomological characterization indicated that this germplasm contains valuable traits of interest for future breeding programs.https://www.mdpi.com/2073-4395/15/1/113<i>Malus domestica</i>SSR markersSNPsex situ collectiondiversity |
| spellingShingle | Danijel Čiček Almira Konjić Martina Skendrović Babojelić Predrag Vujević Silvio Šimon Fuad Gaši Genetic Uniqueness and Pomological Diversity Among the Apple Accessions Maintained Within the Croatian National Clonal Germplasm Repository Agronomy <i>Malus domestica</i> SSR markers SNPs ex situ collection diversity |
| title | Genetic Uniqueness and Pomological Diversity Among the Apple Accessions Maintained Within the Croatian National Clonal Germplasm Repository |
| title_full | Genetic Uniqueness and Pomological Diversity Among the Apple Accessions Maintained Within the Croatian National Clonal Germplasm Repository |
| title_fullStr | Genetic Uniqueness and Pomological Diversity Among the Apple Accessions Maintained Within the Croatian National Clonal Germplasm Repository |
| title_full_unstemmed | Genetic Uniqueness and Pomological Diversity Among the Apple Accessions Maintained Within the Croatian National Clonal Germplasm Repository |
| title_short | Genetic Uniqueness and Pomological Diversity Among the Apple Accessions Maintained Within the Croatian National Clonal Germplasm Repository |
| title_sort | genetic uniqueness and pomological diversity among the apple accessions maintained within the croatian national clonal germplasm repository |
| topic | <i>Malus domestica</i> SSR markers SNPs ex situ collection diversity |
| url | https://www.mdpi.com/2073-4395/15/1/113 |
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