Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates
Polydimethylsiloxane (PDMS) is a commonly used material for the design of new cell culture devices. Typically, molded PDMS is bonded to glass or another PDMS layer; however, it is challenging to form high-strength bonds with polymers commonly used for cell culture-ware, such as polystyrene (PS). The...
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| Format: | Article |
| Language: | English |
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AIP Publishing LLC
2025-01-01
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| Series: | APL Materials |
| Online Access: | http://dx.doi.org/10.1063/5.0249862 |
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| author | Marta K. Orlowska Mary Lor Rebecca L. Fitzsimmons Harley Robinson Iliana Delcheva Daniel Williams Craig Priest James E. Hudson |
| author_facet | Marta K. Orlowska Mary Lor Rebecca L. Fitzsimmons Harley Robinson Iliana Delcheva Daniel Williams Craig Priest James E. Hudson |
| author_sort | Marta K. Orlowska |
| collection | DOAJ |
| description | Polydimethylsiloxane (PDMS) is a commonly used material for the design of new cell culture devices. Typically, molded PDMS is bonded to glass or another PDMS layer; however, it is challenging to form high-strength bonds with polymers commonly used for cell culture-ware, such as polystyrene (PS). Therefore, a robust PDMS–PS bonding method would facilitate a wider use of PDMS in tissue culture applications. Herein, we develop an irreversible, stable, and consistent PDMS–PS bonding protocol. We show that this method is superior to other currently available methods because of its bonding strength, reusability, and reliability. Furthermore, we demonstrate the utility of this bonding process in fabricating our Heart-Dyno device, a 96-well-based platform for culturing cardiac organoids. This bonding process has been successfully used over the past 2 years, with hundreds of plates manufactured for drug screening applications. Others may also have similar applications where this method may facilitate the fabrication of more robust PDMS–PS devices. |
| format | Article |
| id | doaj-art-b53eeb664814493ea94119cc80e0fc1e |
| institution | Kabale University |
| issn | 2166-532X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | AIP Publishing LLC |
| record_format | Article |
| series | APL Materials |
| spelling | doaj-art-b53eeb664814493ea94119cc80e0fc1e2025-02-03T16:42:32ZengAIP Publishing LLCAPL Materials2166-532X2025-01-01131011127011127-1010.1063/5.0249862Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture platesMarta K. Orlowska0Mary Lor1Rebecca L. Fitzsimmons2Harley Robinson3Iliana Delcheva4Daniel Williams5Craig Priest6James E. Hudson7Cardiac Bioengineering Laboratory, QIMR Berghofer Medical Research Institute, Herston, Queensland, AustraliaCardiac Bioengineering Laboratory, QIMR Berghofer Medical Research Institute, Herston, Queensland, AustraliaCardiac Bioengineering Laboratory, QIMR Berghofer Medical Research Institute, Herston, Queensland, AustraliaCardiac Bioengineering Laboratory, QIMR Berghofer Medical Research Institute, Herston, Queensland, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, South Australia, AustraliaCardiac Bioengineering Laboratory, QIMR Berghofer Medical Research Institute, Herston, Queensland, AustraliaPolydimethylsiloxane (PDMS) is a commonly used material for the design of new cell culture devices. Typically, molded PDMS is bonded to glass or another PDMS layer; however, it is challenging to form high-strength bonds with polymers commonly used for cell culture-ware, such as polystyrene (PS). Therefore, a robust PDMS–PS bonding method would facilitate a wider use of PDMS in tissue culture applications. Herein, we develop an irreversible, stable, and consistent PDMS–PS bonding protocol. We show that this method is superior to other currently available methods because of its bonding strength, reusability, and reliability. Furthermore, we demonstrate the utility of this bonding process in fabricating our Heart-Dyno device, a 96-well-based platform for culturing cardiac organoids. This bonding process has been successfully used over the past 2 years, with hundreds of plates manufactured for drug screening applications. Others may also have similar applications where this method may facilitate the fabrication of more robust PDMS–PS devices.http://dx.doi.org/10.1063/5.0249862 |
| spellingShingle | Marta K. Orlowska Mary Lor Rebecca L. Fitzsimmons Harley Robinson Iliana Delcheva Daniel Williams Craig Priest James E. Hudson Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates APL Materials |
| title | Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates |
| title_full | Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates |
| title_fullStr | Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates |
| title_full_unstemmed | Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates |
| title_short | Robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates |
| title_sort | robust and consistent bonding of polydimethylsiloxane to polystyrene cell culture plates |
| url | http://dx.doi.org/10.1063/5.0249862 |
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