Negative Expression of DSG1 and DSG2, as Prognostic Biomarkers, Impacts on the Overall Survival in Patients with Extrahepatic Cholangiocarcinoma
Aims. To evaluate the expression of DSG1 and DSG2 and investigate their clinicopathological significance in EHCC. Method. The protein expression of DSG1 and DSG2 was measured by EnVision immunohistochemistry in 15 normal biliary tract tissues, 10 biliary tract adenoma tissues, 30 peritumoral tissues...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2020/9831646 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832563239274151936 |
|---|---|
| author | Shu Xu Shengfu Huang Daiqiang Li Qiong Zou Yuan Yuan Zhulin Yang |
| author_facet | Shu Xu Shengfu Huang Daiqiang Li Qiong Zou Yuan Yuan Zhulin Yang |
| author_sort | Shu Xu |
| collection | DOAJ |
| description | Aims. To evaluate the expression of DSG1 and DSG2 and investigate their clinicopathological significance in EHCC. Method. The protein expression of DSG1 and DSG2 was measured by EnVision immunohistochemistry in 15 normal biliary tract tissues, 10 biliary tract adenoma tissues, 30 peritumoral tissues, and 100 EHCC tumour tissues. Result. The expression of the DSG1 and DSG2 proteins was significantly lower in EHCC tumour tissues than in normal biliary tract tissues, biliary tract adenoma, and peritumoral tissues (P<0.05). Adenoma and peritumoral tissues with negative DSG1 and/or DSG2 protein expression exhibited atypical hyperplasia. DSG1 expression was positively correlated with DSG2 expression in EHCC (P<0.01). In patients with good differentiation, no invasion, no lymph metastasis, TNM I + II stage, and radical surgery, the positive expression of DSG1 and DSG2 proteins was higher (P<0.05). In comparison to patients with negative DSG1 and/or DSG2 expression, the average overall survival time of those with positive expression was significantly longer (P=0.000). Cox multivariate analysis revealed that negative DSG1 and DSG2 expressions were independent of poor prognosis factors in EHCC patients. The AUC calculated for DSG1 was 0.681 (95% confidence interval: 0.594–0.768) and that for DSG2 was 0.645 (95% confidence interval: 0.555–0.734), while that for DSG1 and DSG2 was 0.772 (95% confidence interval: 0.609-0.936). Conclusions. Negative protein expression of DSG1 and DSG2 is closely related to the pathogenesis, severe clinicopathological characteristics, aggressive biological behaviours, and dismal prognosis in EHCC. |
| format | Article |
| id | doaj-art-b4f9f39d2db54e49b33029ab6592a60b |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-b4f9f39d2db54e49b33029ab6592a60b2025-02-03T01:20:37ZengWileyAnalytical Cellular Pathology2210-71772210-71852020-01-01202010.1155/2020/98316469831646Negative Expression of DSG1 and DSG2, as Prognostic Biomarkers, Impacts on the Overall Survival in Patients with Extrahepatic CholangiocarcinomaShu Xu0Shengfu Huang1Daiqiang Li2Qiong Zou3Yuan Yuan4Zhulin Yang5Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pathology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Pathology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, ChinaDepartment of Pathology, The Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, ChinaDepartment of General Surgery, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaAims. To evaluate the expression of DSG1 and DSG2 and investigate their clinicopathological significance in EHCC. Method. The protein expression of DSG1 and DSG2 was measured by EnVision immunohistochemistry in 15 normal biliary tract tissues, 10 biliary tract adenoma tissues, 30 peritumoral tissues, and 100 EHCC tumour tissues. Result. The expression of the DSG1 and DSG2 proteins was significantly lower in EHCC tumour tissues than in normal biliary tract tissues, biliary tract adenoma, and peritumoral tissues (P<0.05). Adenoma and peritumoral tissues with negative DSG1 and/or DSG2 protein expression exhibited atypical hyperplasia. DSG1 expression was positively correlated with DSG2 expression in EHCC (P<0.01). In patients with good differentiation, no invasion, no lymph metastasis, TNM I + II stage, and radical surgery, the positive expression of DSG1 and DSG2 proteins was higher (P<0.05). In comparison to patients with negative DSG1 and/or DSG2 expression, the average overall survival time of those with positive expression was significantly longer (P=0.000). Cox multivariate analysis revealed that negative DSG1 and DSG2 expressions were independent of poor prognosis factors in EHCC patients. The AUC calculated for DSG1 was 0.681 (95% confidence interval: 0.594–0.768) and that for DSG2 was 0.645 (95% confidence interval: 0.555–0.734), while that for DSG1 and DSG2 was 0.772 (95% confidence interval: 0.609-0.936). Conclusions. Negative protein expression of DSG1 and DSG2 is closely related to the pathogenesis, severe clinicopathological characteristics, aggressive biological behaviours, and dismal prognosis in EHCC.http://dx.doi.org/10.1155/2020/9831646 |
| spellingShingle | Shu Xu Shengfu Huang Daiqiang Li Qiong Zou Yuan Yuan Zhulin Yang Negative Expression of DSG1 and DSG2, as Prognostic Biomarkers, Impacts on the Overall Survival in Patients with Extrahepatic Cholangiocarcinoma Analytical Cellular Pathology |
| title | Negative Expression of DSG1 and DSG2, as Prognostic Biomarkers, Impacts on the Overall Survival in Patients with Extrahepatic Cholangiocarcinoma |
| title_full | Negative Expression of DSG1 and DSG2, as Prognostic Biomarkers, Impacts on the Overall Survival in Patients with Extrahepatic Cholangiocarcinoma |
| title_fullStr | Negative Expression of DSG1 and DSG2, as Prognostic Biomarkers, Impacts on the Overall Survival in Patients with Extrahepatic Cholangiocarcinoma |
| title_full_unstemmed | Negative Expression of DSG1 and DSG2, as Prognostic Biomarkers, Impacts on the Overall Survival in Patients with Extrahepatic Cholangiocarcinoma |
| title_short | Negative Expression of DSG1 and DSG2, as Prognostic Biomarkers, Impacts on the Overall Survival in Patients with Extrahepatic Cholangiocarcinoma |
| title_sort | negative expression of dsg1 and dsg2 as prognostic biomarkers impacts on the overall survival in patients with extrahepatic cholangiocarcinoma |
| url | http://dx.doi.org/10.1155/2020/9831646 |
| work_keys_str_mv | AT shuxu negativeexpressionofdsg1anddsg2asprognosticbiomarkersimpactsontheoverallsurvivalinpatientswithextrahepaticcholangiocarcinoma AT shengfuhuang negativeexpressionofdsg1anddsg2asprognosticbiomarkersimpactsontheoverallsurvivalinpatientswithextrahepaticcholangiocarcinoma AT daiqiangli negativeexpressionofdsg1anddsg2asprognosticbiomarkersimpactsontheoverallsurvivalinpatientswithextrahepaticcholangiocarcinoma AT qiongzou negativeexpressionofdsg1anddsg2asprognosticbiomarkersimpactsontheoverallsurvivalinpatientswithextrahepaticcholangiocarcinoma AT yuanyuan negativeexpressionofdsg1anddsg2asprognosticbiomarkersimpactsontheoverallsurvivalinpatientswithextrahepaticcholangiocarcinoma AT zhulinyang negativeexpressionofdsg1anddsg2asprognosticbiomarkersimpactsontheoverallsurvivalinpatientswithextrahepaticcholangiocarcinoma |