Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro Study
Treating the surfaces of dental implants in an alkaline medium allows us to obtain microstructures of sodium titanate crystals that favor the appearance of apatite in the physiological environment, producing osteoconductive surfaces. In this research, 385 discs made of titanium used in dental implan...
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2025-01-01
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| author | Saray Fernández-Hernández Javier Gil Daniel Robles-Cantero Esteban Pérez-Pevida Mariano Herrero-Climent Aritza Brizuela-Velasco |
| author_facet | Saray Fernández-Hernández Javier Gil Daniel Robles-Cantero Esteban Pérez-Pevida Mariano Herrero-Climent Aritza Brizuela-Velasco |
| author_sort | Saray Fernández-Hernández |
| collection | DOAJ |
| description | Treating the surfaces of dental implants in an alkaline medium allows us to obtain microstructures of sodium titanate crystals that favor the appearance of apatite in the physiological environment, producing osteoconductive surfaces. In this research, 385 discs made of titanium used in dental implants underwent different NaOH treatments with a 6M concentration at 600 °C and cooling rates of 20, 50, 75, and 115 °C/h. Using high-resolution electron microscopy, the microstructures were observed, and the different crystal sizes were determined and compared with control samples (those without biomimetic treatment). Roughness, wettability, surface energy and the sodium content of the surface were determined. The different surfaces were cultured with human osteoblastic cells; cell adhesion was determined at 3 and 14 days, and the degree of mineralization was determined at 14 days via alkaline phosphatase levels. Variations in the microstructure and size of sodium titanate crystals in NaOH solutions rich (1 g/L) or low in calcium (approximately 100 ppm) were determined. The results show that as the cooling rate increases, the size of the crystals decreases (from 0.4 μm to 0.8 μm) except for the case of 115 °C/h, when the rate is too fast for crystalline nucleation to occur on the surface of the titanium. The thermochemical treatment does not influence the roughness or the cooling rate since a Sa of 0.21 μm is maintained. However, the presence of titanate causes a decrease in the contact angle from 70° to 42° and, in turn, causes an increase in the total surface energy from 35 to 49.5 mJ/m<sup>2</sup>, with the polar component standing out in this energy increase. No variations were observed in the thermochemical treatments in the presence of sodium, which was around 1200 ppm. It was observed that as the size of the crystals decreases, cell adhesion increases at 3 days and decreases at 14 days. This is because finer crystals on the surface are already in the mineralization process, as demonstrated using the level of alkaline phosphatase that is maximal for the cooling rate of 75 °C/h. It was possible to confirm that the variations in the concentrated NaOH solutions with different calcium contents did not affect the crystal sizes or the microstructure of the surface. This research makes it possible to obtain dental implants with different mineralization speeds depending on the cooling rate applied. |
| format | Article |
| id | doaj-art-b48489dbd2944f9e97575fed7fcd7538 |
| institution | Kabale University |
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| spelling | doaj-art-b48489dbd2944f9e97575fed7fcd75382025-01-24T13:24:42ZengMDPI AGBiomimetics2313-76732025-01-011014310.3390/biomimetics10010043Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro StudySaray Fernández-Hernández0Javier Gil1Daniel Robles-Cantero2Esteban Pérez-Pevida3Mariano Herrero-Climent4Aritza Brizuela-Velasco5Bioengineering Institute of Technology, Facultad de Medicina y Ciencias de la Salud, Universidad Internacional de Catalunya, C/Josep Trueta s/n, 08195 Sant Cugat del Vallés, SpainBioengineering Institute of Technology, Facultad de Medicina y Ciencias de la Salud, Universidad Internacional de Catalunya, C/Josep Trueta s/n, 08195 Sant Cugat del Vallés, SpainDENS-ia Research Group, Faculty of Health Sciences, Miguel de Cervantes European University, C/del Padre Julio Chevalier 2, 47012 Valladolid, SpainDENS-ia Research Group, Faculty of Health Sciences, Miguel de Cervantes European University, C/del Padre Julio Chevalier 2, 47012 Valladolid, SpainIndependent Researcher, Porto Dental Institute, Av. De Montevideo 810, 4150-518 Oporto, PortugalDENS-ia Research Group, Faculty of Health Sciences, Miguel de Cervantes European University, C/del Padre Julio Chevalier 2, 47012 Valladolid, SpainTreating the surfaces of dental implants in an alkaline medium allows us to obtain microstructures of sodium titanate crystals that favor the appearance of apatite in the physiological environment, producing osteoconductive surfaces. In this research, 385 discs made of titanium used in dental implants underwent different NaOH treatments with a 6M concentration at 600 °C and cooling rates of 20, 50, 75, and 115 °C/h. Using high-resolution electron microscopy, the microstructures were observed, and the different crystal sizes were determined and compared with control samples (those without biomimetic treatment). Roughness, wettability, surface energy and the sodium content of the surface were determined. The different surfaces were cultured with human osteoblastic cells; cell adhesion was determined at 3 and 14 days, and the degree of mineralization was determined at 14 days via alkaline phosphatase levels. Variations in the microstructure and size of sodium titanate crystals in NaOH solutions rich (1 g/L) or low in calcium (approximately 100 ppm) were determined. The results show that as the cooling rate increases, the size of the crystals decreases (from 0.4 μm to 0.8 μm) except for the case of 115 °C/h, when the rate is too fast for crystalline nucleation to occur on the surface of the titanium. The thermochemical treatment does not influence the roughness or the cooling rate since a Sa of 0.21 μm is maintained. However, the presence of titanate causes a decrease in the contact angle from 70° to 42° and, in turn, causes an increase in the total surface energy from 35 to 49.5 mJ/m<sup>2</sup>, with the polar component standing out in this energy increase. No variations were observed in the thermochemical treatments in the presence of sodium, which was around 1200 ppm. It was observed that as the size of the crystals decreases, cell adhesion increases at 3 days and decreases at 14 days. This is because finer crystals on the surface are already in the mineralization process, as demonstrated using the level of alkaline phosphatase that is maximal for the cooling rate of 75 °C/h. It was possible to confirm that the variations in the concentrated NaOH solutions with different calcium contents did not affect the crystal sizes or the microstructure of the surface. This research makes it possible to obtain dental implants with different mineralization speeds depending on the cooling rate applied.https://www.mdpi.com/2313-7673/10/1/43dental implantstitaniumsodium titanatebiomimetic surfaceosteoblasts |
| spellingShingle | Saray Fernández-Hernández Javier Gil Daniel Robles-Cantero Esteban Pérez-Pevida Mariano Herrero-Climent Aritza Brizuela-Velasco Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro Study Biomimetics dental implants titanium sodium titanate biomimetic surface osteoblasts |
| title | Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro Study |
| title_full | Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro Study |
| title_fullStr | Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro Study |
| title_full_unstemmed | Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro Study |
| title_short | Influence of the Sodium Titanate Crystal Size of Biomimetic Dental Implants on Osteoblastic Behavior: An In Vitro Study |
| title_sort | influence of the sodium titanate crystal size of biomimetic dental implants on osteoblastic behavior an in vitro study |
| topic | dental implants titanium sodium titanate biomimetic surface osteoblasts |
| url | https://www.mdpi.com/2313-7673/10/1/43 |
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