Selective inhibition by antiflamrnin-2 of thromboxane B2 release from isolated and perfused guinea-pig lung
Antiflammin-2 (AF2) is a nonapeptide corresponding to the amino acid residues 246–254 of lipocortin-1 showing anti-inflammatory activity both in vitro and in vivo. The effect of AF2 on the thromboxane B2 (TXB2) and histamine release from isolated and perfused guinea-pig lungs has been studied. AF-2...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1992-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935192000383 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832555877570183168 |
|---|---|
| author | Lidia Sautebin Giuseppe Cirino Massimo Di Rosa |
| author_facet | Lidia Sautebin Giuseppe Cirino Massimo Di Rosa |
| author_sort | Lidia Sautebin |
| collection | DOAJ |
| description | Antiflammin-2 (AF2) is a nonapeptide corresponding to the amino acid residues 246–254 of lipocortin-1 showing anti-inflammatory activity both in vitro and in vivo. The effect of AF2 on the thromboxane B2 (TXB2) and histamine release from isolated and perfused guinea-pig lungs has been studied. AF-2 (10–100 nM) inhibited leukotriene C4- (LTC4) (3 ng) and antigen-induced (ovalbumin, 1 mg) TXB2 release in normal and sensitized lungs, respectively. In contrast AF-2 (100 nM) did not modify TXB2 release induced by histamine (5 μg) or bradykinin (5 μg) in normal lungs. Antigen-induced histamine release was not affected by 100 nM AF-2 infusion. When tested in chopped lung fragments AF-2 (0.1–25 μM) did not modify the release of histamine and TXB2 induced by antigen (ovalbumin, 10 μg ml−1) or calcium ionophore A 23187 (1 μM). Our results show that the inhibitory effect of AF-2 on TXB2 release is selective and depends on the stimulus applied. In this respect AF-2 mimics, at least in part, the actions of both glucocorticoids and lipocortin-1. |
| format | Article |
| id | doaj-art-b459cc0bb04046dc8604a5b2506c3733 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b459cc0bb04046dc8604a5b2506c37332025-02-03T05:46:59ZengWileyMediators of Inflammation0962-93511466-18611992-01-011425125410.1155/S0962935192000383Selective inhibition by antiflamrnin-2 of thromboxane B2 release from isolated and perfused guinea-pig lungLidia Sautebin0Giuseppe Cirino1Massimo Di Rosa2Department of Experimental Pharmacology, University of Naples Federico II, via Domenico Montesano 49, Naples 80131, ItalyDepartment of Experimental Pharmacology, University of Naples Federico II, via Domenico Montesano 49, Naples 80131, ItalyDepartment of Experimental Pharmacology, University of Naples Federico II, via Domenico Montesano 49, Naples 80131, ItalyAntiflammin-2 (AF2) is a nonapeptide corresponding to the amino acid residues 246–254 of lipocortin-1 showing anti-inflammatory activity both in vitro and in vivo. The effect of AF2 on the thromboxane B2 (TXB2) and histamine release from isolated and perfused guinea-pig lungs has been studied. AF-2 (10–100 nM) inhibited leukotriene C4- (LTC4) (3 ng) and antigen-induced (ovalbumin, 1 mg) TXB2 release in normal and sensitized lungs, respectively. In contrast AF-2 (100 nM) did not modify TXB2 release induced by histamine (5 μg) or bradykinin (5 μg) in normal lungs. Antigen-induced histamine release was not affected by 100 nM AF-2 infusion. When tested in chopped lung fragments AF-2 (0.1–25 μM) did not modify the release of histamine and TXB2 induced by antigen (ovalbumin, 10 μg ml−1) or calcium ionophore A 23187 (1 μM). Our results show that the inhibitory effect of AF-2 on TXB2 release is selective and depends on the stimulus applied. In this respect AF-2 mimics, at least in part, the actions of both glucocorticoids and lipocortin-1.http://dx.doi.org/10.1155/S0962935192000383 |
| spellingShingle | Lidia Sautebin Giuseppe Cirino Massimo Di Rosa Selective inhibition by antiflamrnin-2 of thromboxane B2 release from isolated and perfused guinea-pig lung Mediators of Inflammation |
| title | Selective inhibition by antiflamrnin-2 of thromboxane B2 release from isolated and perfused guinea-pig lung |
| title_full | Selective inhibition by antiflamrnin-2 of thromboxane B2 release from isolated and perfused guinea-pig lung |
| title_fullStr | Selective inhibition by antiflamrnin-2 of thromboxane B2 release from isolated and perfused guinea-pig lung |
| title_full_unstemmed | Selective inhibition by antiflamrnin-2 of thromboxane B2 release from isolated and perfused guinea-pig lung |
| title_short | Selective inhibition by antiflamrnin-2 of thromboxane B2 release from isolated and perfused guinea-pig lung |
| title_sort | selective inhibition by antiflamrnin 2 of thromboxane b2 release from isolated and perfused guinea pig lung |
| url | http://dx.doi.org/10.1155/S0962935192000383 |
| work_keys_str_mv | AT lidiasautebin selectiveinhibitionbyantiflamrnin2ofthromboxaneb2releasefromisolatedandperfusedguineapiglung AT giuseppecirino selectiveinhibitionbyantiflamrnin2ofthromboxaneb2releasefromisolatedandperfusedguineapiglung AT massimodirosa selectiveinhibitionbyantiflamrnin2ofthromboxaneb2releasefromisolatedandperfusedguineapiglung |