Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo
Oral squamous cell carcinoma (OSCC) is the one of the most common types of malignant tumor found in the head and neck area. Replication factor C subunit 4 (RFC4), an oncogene active in various human cancers, has been rarely studied in OSCC. In the present study, bioinformatics analysis identified RF...
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| Format: | Article |
| Language: | English |
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Wiley
2025-02-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13929 |
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| author | Pengyue You Di Wang Zheng Liu Shuzhen Guan Ning Xiao Haotian Chen Xin Zhang Lichuan Wu Guizhen Wang Haitao Dong |
| author_facet | Pengyue You Di Wang Zheng Liu Shuzhen Guan Ning Xiao Haotian Chen Xin Zhang Lichuan Wu Guizhen Wang Haitao Dong |
| author_sort | Pengyue You |
| collection | DOAJ |
| description | Oral squamous cell carcinoma (OSCC) is the one of the most common types of malignant tumor found in the head and neck area. Replication factor C subunit 4 (RFC4), an oncogene active in various human cancers, has been rarely studied in OSCC. In the present study, bioinformatics analysis identified RFC4 as a potential key target in OSCC progression. Additional experiments showed that RFC4 expression was significantly higher in OSCC tumor tissues than in normal tissues. Knockdown of RFC4 led to G2/M phase cell cycle arrest and inhibited the proliferation of OSCC cells both in vitro and in vivo. High RFC4 expression in OSCC tumors was linked to increased levels of MET, along with reduced levels of CD274 and CD160. Overall, the present study reveals that RFC4 may play a pivotal role in OSCC tumorigenesis and could serve as a potential predictive marker for the efficacy of immunotherapy. |
| format | Article |
| id | doaj-art-b45470d90b4f456ab476ede0835bf7c3 |
| institution | Kabale University |
| issn | 2211-5463 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj-art-b45470d90b4f456ab476ede0835bf7c32025-02-03T10:59:31ZengWileyFEBS Open Bio2211-54632025-02-0115234635810.1002/2211-5463.13929Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivoPengyue You0Di Wang1Zheng Liu2Shuzhen Guan3Ning Xiao4Haotian Chen5Xin Zhang6Lichuan Wu7Guizhen Wang8Haitao Dong9Department of Stomatology, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Clinical Laboratory, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaState Key Laboratory of Molecular Oncology, Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaMedical College of Guangxi University Nanning ChinaDepartment of Stomatology, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Stomatology, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Stomatology, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaMedical College of Guangxi University Nanning ChinaState Key Laboratory of Molecular Oncology, Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaDepartment of Stomatology, Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing ChinaOral squamous cell carcinoma (OSCC) is the one of the most common types of malignant tumor found in the head and neck area. Replication factor C subunit 4 (RFC4), an oncogene active in various human cancers, has been rarely studied in OSCC. In the present study, bioinformatics analysis identified RFC4 as a potential key target in OSCC progression. Additional experiments showed that RFC4 expression was significantly higher in OSCC tumor tissues than in normal tissues. Knockdown of RFC4 led to G2/M phase cell cycle arrest and inhibited the proliferation of OSCC cells both in vitro and in vivo. High RFC4 expression in OSCC tumors was linked to increased levels of MET, along with reduced levels of CD274 and CD160. Overall, the present study reveals that RFC4 may play a pivotal role in OSCC tumorigenesis and could serve as a potential predictive marker for the efficacy of immunotherapy.https://doi.org/10.1002/2211-5463.13929immunotherapyoral squamous cell carcinomaproliferationRFC4WGCNA |
| spellingShingle | Pengyue You Di Wang Zheng Liu Shuzhen Guan Ning Xiao Haotian Chen Xin Zhang Lichuan Wu Guizhen Wang Haitao Dong Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo FEBS Open Bio immunotherapy oral squamous cell carcinoma proliferation RFC4 WGCNA |
| title | Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo |
| title_full | Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo |
| title_fullStr | Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo |
| title_full_unstemmed | Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo |
| title_short | Knockdown of RFC4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo |
| title_sort | knockdown of rfc4 inhibits cell proliferation of oral squamous cell carcinoma in vitro and in vivo |
| topic | immunotherapy oral squamous cell carcinoma proliferation RFC4 WGCNA |
| url | https://doi.org/10.1002/2211-5463.13929 |
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