Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in Arabidopsis
Abstract The Cysteine‐rich secretory proteins (CRISPS), Antigen 5 (Ag5), and Pathogenesis‐related 1 (PR‐1) protein (CAP) superfamily members are found in multiple eukaryotic organisms, including yeasts, animals, and plants. Although one of the plant CAP family genes, PR‐1 is known to respond to path...
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| Format: | Article |
| Language: | English |
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Wiley
2024-10-01
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| Series: | Plant Direct |
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| Online Access: | https://doi.org/10.1002/pld3.70003 |
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| author | Megumi Matsuzawa Takumi Nakayama Masa H. Sato Tomoko Hirano |
| author_facet | Megumi Matsuzawa Takumi Nakayama Masa H. Sato Tomoko Hirano |
| author_sort | Megumi Matsuzawa |
| collection | DOAJ |
| description | Abstract The Cysteine‐rich secretory proteins (CRISPS), Antigen 5 (Ag5), and Pathogenesis‐related 1 (PR‐1) protein (CAP) superfamily members are found in multiple eukaryotic organisms, including yeasts, animals, and plants. Although one of the plant CAP family genes, PR‐1 is known to respond to pathogen infection in plants, the functions of other CAP family genes in Arabidopsis remain largely unknown. In this study, we conducted a comprehensive analysis of the similarities, loci, and expression patterns of 22 Arabidopsis CAP genes/proteins, providing a clue to elucidate their molecular functions. According to the promoter‐β‐glucuronidase (GUS) analysis, members of the Arabidopsis CAP family were expressed in various young tissues or organs, such as root and shoot meristems, reproductive tissues, and particularly at the lateral root initiation site before the formation of the lateral root primordium, with distinct expression specificity. In particular, CAP51, CAP52, and CAP53 were specifically expressed in the cortical cells at the lateral root developing regions, suggesting that these genes may function in lateral root development. Thus, the expression patterns of Arabidopsis CAP family genes suggest that CAP family proteins may have certain function in the expressed organs or tissues in Arabidopsis plant. |
| format | Article |
| id | doaj-art-b44aa716c5394c4bb370d4117c565018 |
| institution | Kabale University |
| issn | 2475-4455 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj-art-b44aa716c5394c4bb370d4117c5650182025-02-04T08:31:56ZengWileyPlant Direct2475-44552024-10-01810n/an/a10.1002/pld3.70003Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in ArabidopsisMegumi Matsuzawa0Takumi Nakayama1Masa H. Sato2Tomoko Hirano3Graduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto JapanGraduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto JapanGraduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto JapanGraduate School of Life and Environmental Sciences Kyoto Prefectural University Kyoto JapanAbstract The Cysteine‐rich secretory proteins (CRISPS), Antigen 5 (Ag5), and Pathogenesis‐related 1 (PR‐1) protein (CAP) superfamily members are found in multiple eukaryotic organisms, including yeasts, animals, and plants. Although one of the plant CAP family genes, PR‐1 is known to respond to pathogen infection in plants, the functions of other CAP family genes in Arabidopsis remain largely unknown. In this study, we conducted a comprehensive analysis of the similarities, loci, and expression patterns of 22 Arabidopsis CAP genes/proteins, providing a clue to elucidate their molecular functions. According to the promoter‐β‐glucuronidase (GUS) analysis, members of the Arabidopsis CAP family were expressed in various young tissues or organs, such as root and shoot meristems, reproductive tissues, and particularly at the lateral root initiation site before the formation of the lateral root primordium, with distinct expression specificity. In particular, CAP51, CAP52, and CAP53 were specifically expressed in the cortical cells at the lateral root developing regions, suggesting that these genes may function in lateral root development. Thus, the expression patterns of Arabidopsis CAP family genes suggest that CAP family proteins may have certain function in the expressed organs or tissues in Arabidopsis plant.https://doi.org/10.1002/pld3.70003antigen 5 (Ag5)Arabidopsiscysteine‐rich secretory proteins (CRISPS)gene expression patternpathogenesis‐related 1 (PR‐1) |
| spellingShingle | Megumi Matsuzawa Takumi Nakayama Masa H. Sato Tomoko Hirano Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in Arabidopsis Plant Direct antigen 5 (Ag5) Arabidopsis cysteine‐rich secretory proteins (CRISPS) gene expression pattern pathogenesis‐related 1 (PR‐1) |
| title | Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in Arabidopsis |
| title_full | Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in Arabidopsis |
| title_fullStr | Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in Arabidopsis |
| title_full_unstemmed | Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in Arabidopsis |
| title_short | Systematic expression analysis of cysteine‐rich secretory proteins, antigen 5, and pathogenesis‐related 1 protein (CAP) superfamily in Arabidopsis |
| title_sort | systematic expression analysis of cysteine rich secretory proteins antigen 5 and pathogenesis related 1 protein cap superfamily in arabidopsis |
| topic | antigen 5 (Ag5) Arabidopsis cysteine‐rich secretory proteins (CRISPS) gene expression pattern pathogenesis‐related 1 (PR‐1) |
| url | https://doi.org/10.1002/pld3.70003 |
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