A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth Cycle
This is the first of a series of calorimetric studies designed to characterize and understand survival mechanisms of metal-reducing bacteria isolated from metal-polluted environments. In this paper we introduce a new concept of thermal spectrum of the endothermic melting of complex biological system...
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| Format: | Article |
| Language: | English |
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Wiley
2003-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2003.33 |
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| author | Nugzar G. Bakradze Marina K. Abuladze Victor M. Sokhadze Nina V. Asatiani Nelly A. Sapojnikova Tamara M. Kartvelishvili Emma N. Namchevadze Nelly Y. Tsibakhashvili Leila V. Tabatadze Lia V. Lejava Hoi-Ying Holman |
| author_facet | Nugzar G. Bakradze Marina K. Abuladze Victor M. Sokhadze Nina V. Asatiani Nelly A. Sapojnikova Tamara M. Kartvelishvili Emma N. Namchevadze Nelly Y. Tsibakhashvili Leila V. Tabatadze Lia V. Lejava Hoi-Ying Holman |
| author_sort | Nugzar G. Bakradze |
| collection | DOAJ |
| description | This is the first of a series of calorimetric studies designed to characterize and understand survival mechanisms of metal-reducing bacteria isolated from metal-polluted environments. In this paper we introduce a new concept of thermal spectrum of the endothermic melting of complex biological systems (e.g., proteins, nucleic acids, ribosomes, membrane structures) in intact cells. All thermal spectra measured are thermograms that describe the temperature dependence of heat capacity change of the complex systems of biologically active substances in bacterial cells. This new concept of thermal spectrum was applied to investigate spectral features from intact cells of Cr(VI)-reducer Arthrobacter oxydans at different points of their growth conditions and stages. Over the temperature range of 40–105°C, we observed that spectral changes are particularly significant in the 40–90°C interval. This may correspond to the orderly changes in subcellular structural elements: proteins, ribosomes and RNA, membranes, and various structural elements of the cell wall during different points of the growth cycle and growth conditions. Spectral changes in the 90–105°C region are less pronounced, implicating that the structural composition of DNA-Protein (DNP) complexes may change little. |
| format | Article |
| id | doaj-art-b4400a49647f47b9ae640fe06ad9e502 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2003-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-b4400a49647f47b9ae640fe06ad9e5022025-02-03T01:22:47ZengWileyThe Scientific World Journal1537-744X2003-01-01343244210.1100/tsw.2003.33A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth CycleNugzar G. Bakradze0Marina K. Abuladze1Victor M. Sokhadze2Nina V. Asatiani3Nelly A. Sapojnikova4Tamara M. Kartvelishvili5Emma N. Namchevadze6Nelly Y. Tsibakhashvili7Leila V. Tabatadze8Lia V. Lejava9Hoi-Ying Holman10Department of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaDepartment of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaDepartment of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaDepartment of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaDepartment of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaInstitute of Molecular Biology and Biophysics, AS of Georgia, 14, Gotua st., Tbilisi, 380069, GeorgiaDepartment of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaDepartment of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaDepartment of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaDepartment of Physics of Biological Systems, Andronikashvili Institute of Physics, AS of Georgia, 6 Tamarashvili st., Tbilisi, 380077, GeorgiaLawrence Berkeley National Laboratory, University of California, Berkeley, USAThis is the first of a series of calorimetric studies designed to characterize and understand survival mechanisms of metal-reducing bacteria isolated from metal-polluted environments. In this paper we introduce a new concept of thermal spectrum of the endothermic melting of complex biological systems (e.g., proteins, nucleic acids, ribosomes, membrane structures) in intact cells. All thermal spectra measured are thermograms that describe the temperature dependence of heat capacity change of the complex systems of biologically active substances in bacterial cells. This new concept of thermal spectrum was applied to investigate spectral features from intact cells of Cr(VI)-reducer Arthrobacter oxydans at different points of their growth conditions and stages. Over the temperature range of 40–105°C, we observed that spectral changes are particularly significant in the 40–90°C interval. This may correspond to the orderly changes in subcellular structural elements: proteins, ribosomes and RNA, membranes, and various structural elements of the cell wall during different points of the growth cycle and growth conditions. Spectral changes in the 90–105°C region are less pronounced, implicating that the structural composition of DNA-Protein (DNP) complexes may change little.http://dx.doi.org/10.1100/tsw.2003.33 |
| spellingShingle | Nugzar G. Bakradze Marina K. Abuladze Victor M. Sokhadze Nina V. Asatiani Nelly A. Sapojnikova Tamara M. Kartvelishvili Emma N. Namchevadze Nelly Y. Tsibakhashvili Leila V. Tabatadze Lia V. Lejava Hoi-Ying Holman A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth Cycle The Scientific World Journal |
| title | A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth Cycle |
| title_full | A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth Cycle |
| title_fullStr | A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth Cycle |
| title_full_unstemmed | A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth Cycle |
| title_short | A Calorimetric Characterization of Cr(VI)-Reducing Arthrobacter oxydans at Different Phases of the Cell Growth Cycle |
| title_sort | calorimetric characterization of cr vi reducing arthrobacter oxydans at different phases of the cell growth cycle |
| url | http://dx.doi.org/10.1100/tsw.2003.33 |
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