Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold
Tinnitus is a common auditory disease worldwide; it is estimated that more than 10% of all individuals experience this hearing disorder during their lifetime. Tinnitus is sometimes accompanied by hearing loss. However, hearing loss is not acquired in some other tinnitus generations. In this study, w...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2020/3949161 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550585557057536 |
|---|---|
| author | Wei Zhang Zhe Peng ShuKui Yu Qing-Ling Song Teng-Fei Qu Lu He Ke Liu Shu-Sheng Gong |
| author_facet | Wei Zhang Zhe Peng ShuKui Yu Qing-Ling Song Teng-Fei Qu Lu He Ke Liu Shu-Sheng Gong |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | Tinnitus is a common auditory disease worldwide; it is estimated that more than 10% of all individuals experience this hearing disorder during their lifetime. Tinnitus is sometimes accompanied by hearing loss. However, hearing loss is not acquired in some other tinnitus generations. In this study, we injected adult rats with salicylate sodium (SS) (200 mg/kg/day for 10 days) and found no significant hearing threshold changes at 2, 4, 8, 12, 14, 16, 20, or 24 kHz (all p>0.05). Tinnitus was confirmed in the treated rats via Behaviour Testing of Acoustic Startle Response (ASR) and Gap Prepulse Inhibition Test of Acoustic Startle Reflex (GPIAS). A immunostaining study showed that there is significant loss of anti-CtBP2 puncta (a marker of cochlear inner hair cell (HC) ribbon synapses) in treated animals in apical, middle, and basal turns (all p<0.05). The ABR wave I amplitudes were significantly reduced at 4, 8, 12, 14, 16, and 20 kHz (all p<0.05). No significant losses of outer HCs, inner HCs, or HC cilia were observed (all p>0.05). Thus, our study suggests that loss of cochlear inner HC ribbon synapse after SS exposure is a contributor to the development of tinnitus without changing hearing threshold. |
| format | Article |
| id | doaj-art-b4371b54e98949a589a8116f1f863433 |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-b4371b54e98949a589a8116f1f8634332025-02-03T06:06:29ZengWileyNeural Plasticity2090-59041687-54432020-01-01202010.1155/2020/39491613949161Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing ThresholdWei Zhang0Zhe Peng1ShuKui Yu2Qing-Ling Song3Teng-Fei Qu4Lu He5Ke Liu6Shu-Sheng Gong7Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong an, Beijing 100050, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong an, Beijing 100050, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong an, Beijing 100050, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong an, Beijing 100050, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong an, Beijing 100050, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong an, Beijing 100050, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong an, Beijing 100050, ChinaDepartment of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing City Xi Cheng District Road 95 Yong an, Beijing 100050, ChinaTinnitus is a common auditory disease worldwide; it is estimated that more than 10% of all individuals experience this hearing disorder during their lifetime. Tinnitus is sometimes accompanied by hearing loss. However, hearing loss is not acquired in some other tinnitus generations. In this study, we injected adult rats with salicylate sodium (SS) (200 mg/kg/day for 10 days) and found no significant hearing threshold changes at 2, 4, 8, 12, 14, 16, 20, or 24 kHz (all p>0.05). Tinnitus was confirmed in the treated rats via Behaviour Testing of Acoustic Startle Response (ASR) and Gap Prepulse Inhibition Test of Acoustic Startle Reflex (GPIAS). A immunostaining study showed that there is significant loss of anti-CtBP2 puncta (a marker of cochlear inner hair cell (HC) ribbon synapses) in treated animals in apical, middle, and basal turns (all p<0.05). The ABR wave I amplitudes were significantly reduced at 4, 8, 12, 14, 16, and 20 kHz (all p<0.05). No significant losses of outer HCs, inner HCs, or HC cilia were observed (all p>0.05). Thus, our study suggests that loss of cochlear inner HC ribbon synapse after SS exposure is a contributor to the development of tinnitus without changing hearing threshold.http://dx.doi.org/10.1155/2020/3949161 |
| spellingShingle | Wei Zhang Zhe Peng ShuKui Yu Qing-Ling Song Teng-Fei Qu Lu He Ke Liu Shu-Sheng Gong Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold Neural Plasticity |
| title | Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold |
| title_full | Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold |
| title_fullStr | Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold |
| title_full_unstemmed | Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold |
| title_short | Loss of Cochlear Ribbon Synapse Is a Critical Contributor to Chronic Salicylate Sodium Treatment-Induced Tinnitus without Change Hearing Threshold |
| title_sort | loss of cochlear ribbon synapse is a critical contributor to chronic salicylate sodium treatment induced tinnitus without change hearing threshold |
| url | http://dx.doi.org/10.1155/2020/3949161 |
| work_keys_str_mv | AT weizhang lossofcochlearribbonsynapseisacriticalcontributortochronicsalicylatesodiumtreatmentinducedtinnituswithoutchangehearingthreshold AT zhepeng lossofcochlearribbonsynapseisacriticalcontributortochronicsalicylatesodiumtreatmentinducedtinnituswithoutchangehearingthreshold AT shukuiyu lossofcochlearribbonsynapseisacriticalcontributortochronicsalicylatesodiumtreatmentinducedtinnituswithoutchangehearingthreshold AT qinglingsong lossofcochlearribbonsynapseisacriticalcontributortochronicsalicylatesodiumtreatmentinducedtinnituswithoutchangehearingthreshold AT tengfeiqu lossofcochlearribbonsynapseisacriticalcontributortochronicsalicylatesodiumtreatmentinducedtinnituswithoutchangehearingthreshold AT luhe lossofcochlearribbonsynapseisacriticalcontributortochronicsalicylatesodiumtreatmentinducedtinnituswithoutchangehearingthreshold AT keliu lossofcochlearribbonsynapseisacriticalcontributortochronicsalicylatesodiumtreatmentinducedtinnituswithoutchangehearingthreshold AT shushenggong lossofcochlearribbonsynapseisacriticalcontributortochronicsalicylatesodiumtreatmentinducedtinnituswithoutchangehearingthreshold |