Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant System
Chronic renal failure (CRF) represents a world health problem. Ozone increases the endogenous antioxidant defense system, preserving the cell redox state. The aim of this study is to evaluate the effect of ozone/oxygen mixture in the renal function, morphology, and biochemical parameters, in an expe...
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| Format: | Article |
| Language: | English |
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Wiley
2005-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/MI.2005.221 |
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| author | José Luis Calunga Zullyt B. Zamora Aluet Borrego Sarahí del Río Ernesto Barber Silvia Menéndez Frank Hernández Teresita Montero Dunia Taboada |
| author_facet | José Luis Calunga Zullyt B. Zamora Aluet Borrego Sarahí del Río Ernesto Barber Silvia Menéndez Frank Hernández Teresita Montero Dunia Taboada |
| author_sort | José Luis Calunga |
| collection | DOAJ |
| description | Chronic renal failure (CRF) represents a world health problem. Ozone increases the endogenous antioxidant defense system, preserving the cell redox state. The aim of this study is to evaluate the effect of ozone/oxygen mixture in the renal function, morphology, and biochemical parameters, in an experimental model of CRF (subtotal nephrectomy). Ozone/oxygen mixture was applied daily, by rectal insufflation (0.5 mg/kg) for 15 sessions after the nephrectomy. Renal function was evaluated, as well as different biochemical parameters, at the beginning and at the end of the study (10 weeks). Renal plasmatic flow (RPF), glomerular filtration rate (GFR), the urine excretion index, and the sodium and potassium excretions (as a measurement of tubular function) in the ozone group were similar to those in Sham group. Nevertheless, nephrectomized rats without ozone (positive control group) showed the lowest RPF, GFR, and urine excretion figures, as well as tubular function. Animals treated with ozone showed systolic arterial pressure (SAP) figures lower than those in the positive control group, but higher values compared to Sham group. Serum creatinine values and protein excretion in 24 hours in the ozone group were decreased compared with nephrectomized rats, but were still higher than normal values. Histological study demonstrated that animals treated with ozone showed less number of lesions in comparison with nephrectomized rats. Thiobarbituric acid reactive substances were significantly increased in nephrectomized and ozone-treated nephrectomized rats in comparison with Sham group. In the positive control group, superoxide dismutase (SOD) and catalase (CAT) showed the lowest figures in comparison with the other groups. However, ozone/oxygen mixture induced a significant stimulation in the enzymatic activity of CAT, SOD, and glutathione peroxidase, as well as reduced glutathione in relation with Sham and positive control groups. In this animal model of CRF, ozone rectal administrations produced a delay in the advance of the disease, protecting the kidneys against vascular, hemorheological, and oxidative mechanisms. This behavior suggests ozone therapy has a protective effect on renal tissue by downregulation of the oxidative stress shown in CRF. |
| format | Article |
| id | doaj-art-b4224fea05ab404bbbd7964a3e537b53 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b4224fea05ab404bbbd7964a3e537b532025-02-03T01:24:53ZengWileyMediators of Inflammation0962-93511466-18612005-01-012005422122710.1155/MI.2005.221Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant SystemJosé Luis Calunga0Zullyt B. Zamora1Aluet Borrego2Sarahí del Río3Ernesto Barber4Silvia Menéndez5Frank Hernández6Teresita Montero7Dunia Taboada8Department of Biomedicine, Ozone Research Center, National Center for Scientific Research, PO Box 6414, Havana, CubaDepartment of Biomedicine, Ozone Research Center, National Center for Scientific Research, PO Box 6414, Havana, CubaDepartment of Biomedicine, Ozone Research Center, National Center for Scientific Research, PO Box 6414, Havana, CubaLuis Díaz Soto Military Medicine Institute, Havana, CubaVictoria de Girón Institute of Basic and Preclinical Sciences, Havana 11600, CubaDepartment of Biomedicine, Ozone Research Center, National Center for Scientific Research, PO Box 6414, Havana, CubaDepartment of Biomedicine, Ozone Research Center, National Center for Scientific Research, PO Box 6414, Havana, CubaLuis Díaz Soto Military Medicine Institute, Havana, CubaVictoria de Girón Institute of Basic and Preclinical Sciences, Havana 11600, CubaChronic renal failure (CRF) represents a world health problem. Ozone increases the endogenous antioxidant defense system, preserving the cell redox state. The aim of this study is to evaluate the effect of ozone/oxygen mixture in the renal function, morphology, and biochemical parameters, in an experimental model of CRF (subtotal nephrectomy). Ozone/oxygen mixture was applied daily, by rectal insufflation (0.5 mg/kg) for 15 sessions after the nephrectomy. Renal function was evaluated, as well as different biochemical parameters, at the beginning and at the end of the study (10 weeks). Renal plasmatic flow (RPF), glomerular filtration rate (GFR), the urine excretion index, and the sodium and potassium excretions (as a measurement of tubular function) in the ozone group were similar to those in Sham group. Nevertheless, nephrectomized rats without ozone (positive control group) showed the lowest RPF, GFR, and urine excretion figures, as well as tubular function. Animals treated with ozone showed systolic arterial pressure (SAP) figures lower than those in the positive control group, but higher values compared to Sham group. Serum creatinine values and protein excretion in 24 hours in the ozone group were decreased compared with nephrectomized rats, but were still higher than normal values. Histological study demonstrated that animals treated with ozone showed less number of lesions in comparison with nephrectomized rats. Thiobarbituric acid reactive substances were significantly increased in nephrectomized and ozone-treated nephrectomized rats in comparison with Sham group. In the positive control group, superoxide dismutase (SOD) and catalase (CAT) showed the lowest figures in comparison with the other groups. However, ozone/oxygen mixture induced a significant stimulation in the enzymatic activity of CAT, SOD, and glutathione peroxidase, as well as reduced glutathione in relation with Sham and positive control groups. In this animal model of CRF, ozone rectal administrations produced a delay in the advance of the disease, protecting the kidneys against vascular, hemorheological, and oxidative mechanisms. This behavior suggests ozone therapy has a protective effect on renal tissue by downregulation of the oxidative stress shown in CRF.http://dx.doi.org/10.1155/MI.2005.221 |
| spellingShingle | José Luis Calunga Zullyt B. Zamora Aluet Borrego Sarahí del Río Ernesto Barber Silvia Menéndez Frank Hernández Teresita Montero Dunia Taboada Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant System Mediators of Inflammation |
| title | Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant System |
| title_full | Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant System |
| title_fullStr | Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant System |
| title_full_unstemmed | Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant System |
| title_short | Ozone Therapy on Rats Submitted to Subtotal Nephrectomy: Role of Antioxidant System |
| title_sort | ozone therapy on rats submitted to subtotal nephrectomy role of antioxidant system |
| url | http://dx.doi.org/10.1155/MI.2005.221 |
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