Distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybrids
Yeast cells sometimes engage in interspecific hybridization, i.e., crosses between different species. These interspecific yeast hybrids combine phenotypes of the two parental species and can therefore allow fast adaptation to new niches. This is perhaps most evident in beer yeasts, where a cross bet...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1472832/full |
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| author | Pablo Murath Pablo Murath Stephanie Hoffmann Beatriz Herrera-Malaver Beatriz Herrera-Malaver Beatriz Herrera-Malaver Luis Bustamante Kevin Verstrepen Kevin Verstrepen Kevin Verstrepen Jan Steensels Jan Steensels Jan Steensels |
| author_facet | Pablo Murath Pablo Murath Stephanie Hoffmann Beatriz Herrera-Malaver Beatriz Herrera-Malaver Beatriz Herrera-Malaver Luis Bustamante Kevin Verstrepen Kevin Verstrepen Kevin Verstrepen Jan Steensels Jan Steensels Jan Steensels |
| author_sort | Pablo Murath |
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| description | Yeast cells sometimes engage in interspecific hybridization, i.e., crosses between different species. These interspecific yeast hybrids combine phenotypes of the two parental species and can therefore allow fast adaptation to new niches. This is perhaps most evident in beer yeasts, where a cross between Saccharomyces cerevisiae and Saccharomyces eubayanus led to the emergence of the lager yeast Saccharomyces pastorianus, which combines the fermentation capacity of S. cerevisiae with the cold tolerance of S. eubayanus, making the hybrid suitable for the typical cool lager beer fermentation conditions. Interestingly, however, merging two different genomes into one cell causes genomic instability and rearrangements, ultimately leading to a reorganized but more stable hybrid genome. Here, we investigate how different parameters influence this genome stabilization trajectory and ultimately can lead to variants with different industrial phenotypes. We generated seven de novo interspecific hybrids between two S. eubayanus strains and an ale S. cerevisiae strain, subsequently exposing them to three different genome stabilization procedures. Next, we analyzed the fermentation characteristics and metabolite production of selected stabilized hybrids. Our results reveal how variation in the genome stabilization procedure leads to phenotypic variability and can generate additional diversity after the initial hybridization process. Moreover, several stabilized hybrids showed phenotypes that are interesting for industrial applications. |
| format | Article |
| id | doaj-art-b400c042bd3d4c3795073834325056bd |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-b400c042bd3d4c3795073834325056bd2025-01-29T06:45:47ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.14728321472832Distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybridsPablo Murath0Pablo Murath1Stephanie Hoffmann2Beatriz Herrera-Malaver3Beatriz Herrera-Malaver4Beatriz Herrera-Malaver5Luis Bustamante6Kevin Verstrepen7Kevin Verstrepen8Kevin Verstrepen9Jan Steensels10Jan Steensels11Jan Steensels12Departmento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Concepción, ChileDepartamento de Ciencia y Tecnología de los Alimentos, Facultad de Farmacia, Universidad de Concepción, Concepción, ChileDepartamento de Ciencia y Tecnología de los Alimentos, Facultad de Farmacia, Universidad de Concepción, Concepción, ChileLeuven Institute for Beer Research, Katholieke Universiteit Leuven, Bio-Incubator, Leuven, BelgiumLaboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, BelgiumLaboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Leuven, BelgiumDepartmento de Análisis Instrumental, Facultad de Farmacia, Universidad de Concepción, Concepción, ChileLeuven Institute for Beer Research, Katholieke Universiteit Leuven, Bio-Incubator, Leuven, BelgiumLaboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, BelgiumLaboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Leuven, BelgiumLeuven Institute for Beer Research, Katholieke Universiteit Leuven, Bio-Incubator, Leuven, BelgiumLaboratory for Systems Biology, VIB Centre for Microbiology, Bio-Incubator, Leuven, BelgiumLaboratory for Genetics and Genomics, Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Leuven, BelgiumYeast cells sometimes engage in interspecific hybridization, i.e., crosses between different species. These interspecific yeast hybrids combine phenotypes of the two parental species and can therefore allow fast adaptation to new niches. This is perhaps most evident in beer yeasts, where a cross between Saccharomyces cerevisiae and Saccharomyces eubayanus led to the emergence of the lager yeast Saccharomyces pastorianus, which combines the fermentation capacity of S. cerevisiae with the cold tolerance of S. eubayanus, making the hybrid suitable for the typical cool lager beer fermentation conditions. Interestingly, however, merging two different genomes into one cell causes genomic instability and rearrangements, ultimately leading to a reorganized but more stable hybrid genome. Here, we investigate how different parameters influence this genome stabilization trajectory and ultimately can lead to variants with different industrial phenotypes. We generated seven de novo interspecific hybrids between two S. eubayanus strains and an ale S. cerevisiae strain, subsequently exposing them to three different genome stabilization procedures. Next, we analyzed the fermentation characteristics and metabolite production of selected stabilized hybrids. Our results reveal how variation in the genome stabilization procedure leads to phenotypic variability and can generate additional diversity after the initial hybridization process. Moreover, several stabilized hybrids showed phenotypes that are interesting for industrial applications.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1472832/fullLager yeastinterspecific hybridsgenome stabilizationflavor productionbrewing |
| spellingShingle | Pablo Murath Pablo Murath Stephanie Hoffmann Beatriz Herrera-Malaver Beatriz Herrera-Malaver Beatriz Herrera-Malaver Luis Bustamante Kevin Verstrepen Kevin Verstrepen Kevin Verstrepen Jan Steensels Jan Steensels Jan Steensels Distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybrids Frontiers in Microbiology Lager yeast interspecific hybrids genome stabilization flavor production brewing |
| title | Distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybrids |
| title_full | Distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybrids |
| title_fullStr | Distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybrids |
| title_full_unstemmed | Distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybrids |
| title_short | Distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybrids |
| title_sort | distinct genome stabilization procedures lead to phenotypic variability in newly generated interspecific yeast hybrids |
| topic | Lager yeast interspecific hybrids genome stabilization flavor production brewing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1472832/full |
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