The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitro
The effect of lyso-PAF on ciliated cells was investigated in vitro. Normal mucosa was surgically obtained from human paranasal sinuses and incubated in the form of tissue culture. Ciliated epithelium was magnified under an inverted microscope, and ciliary movement was photo-electrically measured. Ci...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000074 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567610726678528 |
|---|---|
| author | T. Ganbo K. Hisamatsu H. Inoue T. Nakajima Y. Murakami |
| author_facet | T. Ganbo K. Hisamatsu H. Inoue T. Nakajima Y. Murakami |
| author_sort | T. Ganbo |
| collection | DOAJ |
| description | The effect of lyso-PAF on ciliated cells was investigated in vitro.
Normal mucosa was surgically obtained from human paranasal sinuses
and incubated in the form of tissue culture. Ciliated epithelium was
magnified under an inverted microscope, and ciliary movement was
photo-electrically measured. Ciliary activity was significantly
inhibited by 10−8 M lyso-PAF and could be restored. The effect of
lyso-PAF was completely blocked by CV-6209, a specific PAF
antagonist. The PAF concentration in the incubation medium of
lyso-PAF was determined by radioimmunoassay, because PAF is a well
known inhibitor of ciliary activity. PAF gradually increased and
after 20 min reached its maximal level. These findings indicated the
existence of an enzyme in the paranasal sinus mucosa, by which
lyso-PAF is converted to PAF, and that lyso-PAF can inhibit ciliary
activity by means of PAF. |
| format | Article |
| id | doaj-art-b3f6a15232ee4c768e0c128d11e6c831 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b3f6a15232ee4c768e0c128d11e6c8312025-02-03T01:01:09ZengWileyMediators of Inflammation0962-93511466-18611994-01-0131414410.1155/S0962935194000074The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitroT. Ganbo0K. Hisamatsu1H. Inoue2T. Nakajima3Y. Murakami4Department of Otolaryngology, Yamanshi Medical University, 1110 Shimogato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, JapanDepartment of Otolaryngology, Yamanshi Medical University, 1110 Shimogato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, JapanDepartment of Plastic and Reconstructive Surgery, St Marianna University School of Medicine, Kawasaki, Kanagawa, JapanDepartment of Otolaryngology, Yamanshi Medical University, 1110 Shimogato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, JapanDepartment of Otolaryngology, Yamanshi Medical University, 1110 Shimogato, Tamaho-cho, Nakakoma-gun, 409-38, Yamanashi, JapanThe effect of lyso-PAF on ciliated cells was investigated in vitro. Normal mucosa was surgically obtained from human paranasal sinuses and incubated in the form of tissue culture. Ciliated epithelium was magnified under an inverted microscope, and ciliary movement was photo-electrically measured. Ciliary activity was significantly inhibited by 10−8 M lyso-PAF and could be restored. The effect of lyso-PAF was completely blocked by CV-6209, a specific PAF antagonist. The PAF concentration in the incubation medium of lyso-PAF was determined by radioimmunoassay, because PAF is a well known inhibitor of ciliary activity. PAF gradually increased and after 20 min reached its maximal level. These findings indicated the existence of an enzyme in the paranasal sinus mucosa, by which lyso-PAF is converted to PAF, and that lyso-PAF can inhibit ciliary activity by means of PAF.http://dx.doi.org/10.1155/S0962935194000074 |
| spellingShingle | T. Ganbo K. Hisamatsu H. Inoue T. Nakajima Y. Murakami The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitro Mediators of Inflammation |
| title | The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitro |
| title_full | The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitro |
| title_fullStr | The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitro |
| title_full_unstemmed | The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitro |
| title_short | The Effect of Lyso-PAF on Ciliary Activity of Human Paranasal Sinus Mucosa in vitro |
| title_sort | effect of lyso paf on ciliary activity of human paranasal sinus mucosa in vitro |
| url | http://dx.doi.org/10.1155/S0962935194000074 |
| work_keys_str_mv | AT tganbo theeffectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT khisamatsu theeffectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT hinoue theeffectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT tnakajima theeffectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT ymurakami theeffectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT tganbo effectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT khisamatsu effectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT hinoue effectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT tnakajima effectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro AT ymurakami effectoflysopafonciliaryactivityofhumanparanasalsinusmucosainvitro |