Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathway
IntroductionMiR-144-5p is differentially expressed in plasma exosomes from Bactrian camels of varying body sizes, with GO and KEGG analyses indicating that its target genes play crucial roles in lipid metabolism. PGC-1α, confirmed as a key target through RNA pull-down and dual-luciferase reporter as...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1477593/full |
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| author | Siriguleng Yu Siriguleng Yu Chaoqun Liu Hong Lv Zhijun Guo Hongqiang Yao Hongqiang Yao Xin Wen Ying Xiu Wa Gao Ziyi Li Wen Yu Yaru Niu Junjian Jin |
| author_facet | Siriguleng Yu Siriguleng Yu Chaoqun Liu Hong Lv Zhijun Guo Hongqiang Yao Hongqiang Yao Xin Wen Ying Xiu Wa Gao Ziyi Li Wen Yu Yaru Niu Junjian Jin |
| author_sort | Siriguleng Yu |
| collection | DOAJ |
| description | IntroductionMiR-144-5p is differentially expressed in plasma exosomes from Bactrian camels of varying body sizes, with GO and KEGG analyses indicating that its target genes play crucial roles in lipid metabolism. PGC-1α, confirmed as a key target through RNA pull-down and dual-luciferase reporter assays, is a significant regulator of this process. This study aims to investigate the impact of miR-144-5p on lipid metabolism in a mouse model of high-fat diet (HFD)-induced obesity to elucidate the mechanistic pathways involved.MethodsMale C57BL/6 mice were assigned to groups and fed either a control diet or an HFD for 12 weeks, which was followed by a 3-week intervention with miR-144-5p. Various metabolic parameters and gene expressions were evaluated to determine the effects of this treatment.ResultsThe treatment significantly reduced diet-induced adiposity and decreased inflammatory responses. Additionally, it inhibited PGC-1α expression in the liver tissue of HFD-fed mice. There was an increase in CPT1 activity and fatty acid β-oxidation rate, along with the downregulation of FASN mRNA expression and the upregulation of CPT1 and ACOX1 expression, promoting fatty acid oxidation.DiscussionThese findings suggest that miR-144-5p exerts anti-obesity effects by enhancing fatty acid oxidation, likely through modulation of the PGC-1α/AMPK signaling pathway. This study provides new insights into potential miRNA-based therapeutic strategies for obesity and related metabolic disorders. |
| format | Article |
| id | doaj-art-b3ef2fa49c8d40eb8d385fdd8463fc30 |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-b3ef2fa49c8d40eb8d385fdd8463fc302025-08-20T03:41:44ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-08-011210.3389/fvets.2025.14775931477593Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathwaySiriguleng Yu0Siriguleng Yu1Chaoqun Liu2Hong Lv3Zhijun Guo4Hongqiang Yao5Hongqiang Yao6Xin Wen7Ying Xiu8Wa Gao9Ziyi Li10Wen Yu11Yaru Niu12Junjian Jin13College of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaKey Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaKey Laboratory of Clinical Diagnosis and Treatment Technology for Animal Diseases, Ministry of Agriculture, Hohhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaDepartment of Public Health, Inner Mongolia Center for Disease Control and Prevention, Hohhot, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Huhhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaCollege of Veterinary Medicine, Inner Mongolia Agricultural University, Huhhot, ChinaIntroductionMiR-144-5p is differentially expressed in plasma exosomes from Bactrian camels of varying body sizes, with GO and KEGG analyses indicating that its target genes play crucial roles in lipid metabolism. PGC-1α, confirmed as a key target through RNA pull-down and dual-luciferase reporter assays, is a significant regulator of this process. This study aims to investigate the impact of miR-144-5p on lipid metabolism in a mouse model of high-fat diet (HFD)-induced obesity to elucidate the mechanistic pathways involved.MethodsMale C57BL/6 mice were assigned to groups and fed either a control diet or an HFD for 12 weeks, which was followed by a 3-week intervention with miR-144-5p. Various metabolic parameters and gene expressions were evaluated to determine the effects of this treatment.ResultsThe treatment significantly reduced diet-induced adiposity and decreased inflammatory responses. Additionally, it inhibited PGC-1α expression in the liver tissue of HFD-fed mice. There was an increase in CPT1 activity and fatty acid β-oxidation rate, along with the downregulation of FASN mRNA expression and the upregulation of CPT1 and ACOX1 expression, promoting fatty acid oxidation.DiscussionThese findings suggest that miR-144-5p exerts anti-obesity effects by enhancing fatty acid oxidation, likely through modulation of the PGC-1α/AMPK signaling pathway. This study provides new insights into potential miRNA-based therapeutic strategies for obesity and related metabolic disorders.https://www.frontiersin.org/articles/10.3389/fvets.2025.1477593/fullBactrian camelexosomemetabolismmiR-144-5pPGC-1α |
| spellingShingle | Siriguleng Yu Siriguleng Yu Chaoqun Liu Hong Lv Zhijun Guo Hongqiang Yao Hongqiang Yao Xin Wen Ying Xiu Wa Gao Ziyi Li Wen Yu Yaru Niu Junjian Jin Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathway Frontiers in Veterinary Science Bactrian camel exosome metabolism miR-144-5p PGC-1α |
| title | Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathway |
| title_full | Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathway |
| title_fullStr | Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathway |
| title_full_unstemmed | Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathway |
| title_short | Role of miR-144-5p in modulating lipid metabolism and potentially alleviating obesity via the PGC-1α/AMPK pathway |
| title_sort | role of mir 144 5p in modulating lipid metabolism and potentially alleviating obesity via the pgc 1α ampk pathway |
| topic | Bactrian camel exosome metabolism miR-144-5p PGC-1α |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1477593/full |
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