Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression
Aim. The effects of cryopreservation on adipose tissue-derived mesenchymal stem cells are not clearly documented, as there is a growing body of evidence about the importance of adipose-derived mesenchymal stem cells for regenerative therapies. The aim of this study was to analyze human adipose tissu...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/2562718 |
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| author | Ana Carolina Irioda Rafael Cassilha Larissa Zocche Julio Cesar Francisco Ricardo Correa Cunha Priscila Elias Ferreira Luiz Cesar Guarita-Souza Reginaldo Justino Ferreira Bassam Felipe Mogharbel Venkata Naga Srikanth Garikipati Daiany Souza Mirian Perlingeiro Beltrame Katherine Athayde Teixeira de Carvalho |
| author_facet | Ana Carolina Irioda Rafael Cassilha Larissa Zocche Julio Cesar Francisco Ricardo Correa Cunha Priscila Elias Ferreira Luiz Cesar Guarita-Souza Reginaldo Justino Ferreira Bassam Felipe Mogharbel Venkata Naga Srikanth Garikipati Daiany Souza Mirian Perlingeiro Beltrame Katherine Athayde Teixeira de Carvalho |
| author_sort | Ana Carolina Irioda |
| collection | DOAJ |
| description | Aim. The effects of cryopreservation on adipose tissue-derived mesenchymal stem cells are not clearly documented, as there is a growing body of evidence about the importance of adipose-derived mesenchymal stem cells for regenerative therapies. The aim of this study was to analyze human adipose tissue-derived mesenchymal stem cells phenotypic expression (CD34, CD45, CD73, CD90, CD105, and CD49d), colony forming unit ability, viability, and differentiation potential before and after cryopreservation. Materials and Methods. 12 samples of the adipose tissue were collected from a healthy donor using the liposuction technique. The cell isolation was performed by enzymatic digestion and then the cells were cultured up to passage 2. Before and after cryopreservation the immunophenotype, cellular viability analysis by flow cytometer, colony forming units ability, differentiation potential into adipocytes and osteoblasts as demonstrated by Oil Red O and Alizarin Red staining, respectively. Results. The immunophenotypic markers expression was largely preserved, and their multipotency was maintained. However, after cryopreservation, the cells decreased α4-integrin expression (CD49d), cell viability, and number of colony forming units. Conclusions. These findings suggest that ADMSC transplanted after cryopreservation might compromise the retention of transplanted cells in the host tissue. Therefore, further studies are warranted to standardize protocols related to cryopreservation to attain full benefits of stem cell therapy. |
| format | Article |
| id | doaj-art-b3e529a2454847cb904b2b7a841950c5 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b3e529a2454847cb904b2b7a841950c52025-02-03T06:11:41ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/25627182562718Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin ExpressionAna Carolina Irioda0Rafael Cassilha1Larissa Zocche2Julio Cesar Francisco3Ricardo Correa Cunha4Priscila Elias Ferreira5Luiz Cesar Guarita-Souza6Reginaldo Justino Ferreira7Bassam Felipe Mogharbel8Venkata Naga Srikanth Garikipati9Daiany Souza10Mirian Perlingeiro Beltrame11Katherine Athayde Teixeira de Carvalho12Cell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilHospital Santé Curitiba, Plastic Surgery Clinic, Rua XV de Novembro 2913, 80045-340 Curitiba, PR, BrazilCell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilCell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilCell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilCell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilExperimental Laboratory of Institute of Biological and Health Sciences of Pontifical Catholic University of Paraná (PUCPR), Rua Imaculada Conceição 1155, 80215-901 Curitiba, PR, BrazilCell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilCell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilCenter for Translational Medicine, Temple University School of Medicine, Philadelphia, PA 19140, USACell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilFlow Cytometric Analysis Laboratory, Clinical Hospital of Federal University of Paraná, Rua Padre Camargo 280, Alto da Glória, 80060-240 Curitiba, PR, BrazilCell Therapy and Biotechnology in Regenerative Medicine Research Group, Pelé Pequeno Príncipe Institute, Avenida Silva Jardim 1632, 80250-200 Curitiba, PR, BrazilAim. The effects of cryopreservation on adipose tissue-derived mesenchymal stem cells are not clearly documented, as there is a growing body of evidence about the importance of adipose-derived mesenchymal stem cells for regenerative therapies. The aim of this study was to analyze human adipose tissue-derived mesenchymal stem cells phenotypic expression (CD34, CD45, CD73, CD90, CD105, and CD49d), colony forming unit ability, viability, and differentiation potential before and after cryopreservation. Materials and Methods. 12 samples of the adipose tissue were collected from a healthy donor using the liposuction technique. The cell isolation was performed by enzymatic digestion and then the cells were cultured up to passage 2. Before and after cryopreservation the immunophenotype, cellular viability analysis by flow cytometer, colony forming units ability, differentiation potential into adipocytes and osteoblasts as demonstrated by Oil Red O and Alizarin Red staining, respectively. Results. The immunophenotypic markers expression was largely preserved, and their multipotency was maintained. However, after cryopreservation, the cells decreased α4-integrin expression (CD49d), cell viability, and number of colony forming units. Conclusions. These findings suggest that ADMSC transplanted after cryopreservation might compromise the retention of transplanted cells in the host tissue. Therefore, further studies are warranted to standardize protocols related to cryopreservation to attain full benefits of stem cell therapy.http://dx.doi.org/10.1155/2016/2562718 |
| spellingShingle | Ana Carolina Irioda Rafael Cassilha Larissa Zocche Julio Cesar Francisco Ricardo Correa Cunha Priscila Elias Ferreira Luiz Cesar Guarita-Souza Reginaldo Justino Ferreira Bassam Felipe Mogharbel Venkata Naga Srikanth Garikipati Daiany Souza Mirian Perlingeiro Beltrame Katherine Athayde Teixeira de Carvalho Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression Stem Cells International |
| title | Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression |
| title_full | Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression |
| title_fullStr | Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression |
| title_full_unstemmed | Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression |
| title_short | Human Adipose-Derived Mesenchymal Stem Cells Cryopreservation and Thawing Decrease α4-Integrin Expression |
| title_sort | human adipose derived mesenchymal stem cells cryopreservation and thawing decrease α4 integrin expression |
| url | http://dx.doi.org/10.1155/2016/2562718 |
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