Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for Bioproduction
ABSTRACT Glucose is the most abundant monosaccharide and a principal substrate in biotechnological production processes. In Pseudomonas, this sugar is either imported directly into the cytosol or first oxidised to gluconate in the periplasm. While gluconate is taken up via a proton‐driven symporter,...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Microbial Biotechnology |
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| Online Access: | https://doi.org/10.1111/1751-7915.70095 |
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| author | Tobias Schwanemann Nicolas Krink Pablo I. Nikel Benedikt Wynands Nick Wierckx |
| author_facet | Tobias Schwanemann Nicolas Krink Pablo I. Nikel Benedikt Wynands Nick Wierckx |
| author_sort | Tobias Schwanemann |
| collection | DOAJ |
| description | ABSTRACT Glucose is the most abundant monosaccharide and a principal substrate in biotechnological production processes. In Pseudomonas, this sugar is either imported directly into the cytosol or first oxidised to gluconate in the periplasm. While gluconate is taken up via a proton‐driven symporter, the import of glucose is mediated by an ABC‐type transporter, and hence both require energy. In this study, we heterologously expressed the energy‐independent glucose facilitator protein (Glf) from Zymomonas mobilis to replace the native energy‐demanding glucose transport systems, thereby increasing the metabolic energy efficiency. The implementation of passive glucose uptake in engineered production strains significantly increased product titres and yields of the two different aromatic products, cinnamic acid (+10%–15%) and resveratrol (+26%; 18.1 mg/g) in batch cultures. |
| format | Article |
| id | doaj-art-b3de997a3e104094885b3ec22df7a561 |
| institution | Kabale University |
| issn | 1751-7915 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj-art-b3de997a3e104094885b3ec22df7a5612025-01-31T06:26:36ZengWileyMicrobial Biotechnology1751-79152025-01-01181n/an/a10.1111/1751-7915.70095Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for BioproductionTobias Schwanemann0Nicolas Krink1Pablo I. Nikel2Benedikt Wynands3Nick Wierckx4Institute of Bio‐ and Geosciences, IBG‐1: Biotechnology Forschungszentrum Jülich GmbH Jülich GermanyThe Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark Kongens Lyngby DenmarkThe Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark Kongens Lyngby DenmarkInstitute of Bio‐ and Geosciences, IBG‐1: Biotechnology Forschungszentrum Jülich GmbH Jülich GermanyInstitute of Bio‐ and Geosciences, IBG‐1: Biotechnology Forschungszentrum Jülich GmbH Jülich GermanyABSTRACT Glucose is the most abundant monosaccharide and a principal substrate in biotechnological production processes. In Pseudomonas, this sugar is either imported directly into the cytosol or first oxidised to gluconate in the periplasm. While gluconate is taken up via a proton‐driven symporter, the import of glucose is mediated by an ABC‐type transporter, and hence both require energy. In this study, we heterologously expressed the energy‐independent glucose facilitator protein (Glf) from Zymomonas mobilis to replace the native energy‐demanding glucose transport systems, thereby increasing the metabolic energy efficiency. The implementation of passive glucose uptake in engineered production strains significantly increased product titres and yields of the two different aromatic products, cinnamic acid (+10%–15%) and resveratrol (+26%; 18.1 mg/g) in batch cultures.https://doi.org/10.1111/1751-7915.70095ATP consumptionglucose transportmetabolic engineeringPseudomonasstrain optimization |
| spellingShingle | Tobias Schwanemann Nicolas Krink Pablo I. Nikel Benedikt Wynands Nick Wierckx Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for Bioproduction Microbial Biotechnology ATP consumption glucose transport metabolic engineering Pseudomonas strain optimization |
| title | Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for Bioproduction |
| title_full | Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for Bioproduction |
| title_fullStr | Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for Bioproduction |
| title_full_unstemmed | Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for Bioproduction |
| title_short | Engineered Passive Glucose Uptake in Pseudomonas taiwanensis VLB120 Increases Resource Efficiency for Bioproduction |
| title_sort | engineered passive glucose uptake in pseudomonas taiwanensis vlb120 increases resource efficiency for bioproduction |
| topic | ATP consumption glucose transport metabolic engineering Pseudomonas strain optimization |
| url | https://doi.org/10.1111/1751-7915.70095 |
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