Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B1 Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium
Aflatoxin B1 (AFB1) is a carcinogenic metabolite produced by certain Aspergillus species on agricultural commodities. AFB1 biosynthesis is affected by jasmonic acid and also by its methylester (MeJA), a plant growth regulator derived from linoleic acid. This study reports the effect of MeJA on the g...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2009/842626 |
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| author | Dido Maria Meimaroglou Dia Galanopoulou Panagiota Markaki |
| author_facet | Dido Maria Meimaroglou Dia Galanopoulou Panagiota Markaki |
| author_sort | Dido Maria Meimaroglou |
| collection | DOAJ |
| description | Aflatoxin B1 (AFB1) is a carcinogenic metabolite produced by certain Aspergillus species on agricultural commodities. AFB1 biosynthesis is affected by jasmonic acid and also by its methylester (MeJA), a plant growth regulator derived from linoleic acid. This study reports the effect of MeJA on the growth of A. parasiticus and AFB1 output in yeast extract sucrose (YES) medium when added at three different concentrations; namely, 10−2 M, 10−4 M, and 10−6 M. AFB1 determination was performed by immunoaffinity and HPLC. MeJA at 10−4 and 10−6 M concentrations had no significant effect on mycelial growth but did affect AFB1 production after the 7th day of incubation; on the 12th day, AFB1 production was increased by 212.7% and 141.6% compared to the control samples (addition of 10−6 M and 10−4 M MeJA, resp.). Treatment of A. parasiticus cultures with 10−2 M MeJA inhibited mycelial growth and AFB1 production as well. These results suggest that the effect of MeJA on AFB1 biosynthesis by A. parasiticus depends on the MeJA concentration used. |
| format | Article |
| id | doaj-art-b3734cd16815467fa8a5c9f5da368d33 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-b3734cd16815467fa8a5c9f5da368d332025-02-03T01:26:50ZengWileyInternational Journal of Microbiology1687-918X1687-91982009-01-01200910.1155/2009/842626842626Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B1 Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose MediumDido Maria Meimaroglou0Dia Galanopoulou1Panagiota Markaki2Department of Food Chemistry, School of Chemistry, University of Athens, Panepistimiopolis Zogra-fou, 15784 Athens, GreeceLaboratory of Biochemistry, School of Chemistry, University of Athens, Panepistimiopolis Zografou, 15784 Athens, GreeceDepartment of Food Chemistry, School of Chemistry, University of Athens, Panepistimiopolis Zogra-fou, 15784 Athens, GreeceAflatoxin B1 (AFB1) is a carcinogenic metabolite produced by certain Aspergillus species on agricultural commodities. AFB1 biosynthesis is affected by jasmonic acid and also by its methylester (MeJA), a plant growth regulator derived from linoleic acid. This study reports the effect of MeJA on the growth of A. parasiticus and AFB1 output in yeast extract sucrose (YES) medium when added at three different concentrations; namely, 10−2 M, 10−4 M, and 10−6 M. AFB1 determination was performed by immunoaffinity and HPLC. MeJA at 10−4 and 10−6 M concentrations had no significant effect on mycelial growth but did affect AFB1 production after the 7th day of incubation; on the 12th day, AFB1 production was increased by 212.7% and 141.6% compared to the control samples (addition of 10−6 M and 10−4 M MeJA, resp.). Treatment of A. parasiticus cultures with 10−2 M MeJA inhibited mycelial growth and AFB1 production as well. These results suggest that the effect of MeJA on AFB1 biosynthesis by A. parasiticus depends on the MeJA concentration used.http://dx.doi.org/10.1155/2009/842626 |
| spellingShingle | Dido Maria Meimaroglou Dia Galanopoulou Panagiota Markaki Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B1 Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium International Journal of Microbiology |
| title | Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B1 Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium |
| title_full | Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B1 Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium |
| title_fullStr | Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B1 Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium |
| title_full_unstemmed | Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B1 Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium |
| title_short | Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B1 Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium |
| title_sort | study of the effect of methyl jasmonate concentration on aflatoxin b1 biosynthesis by aspergillus parasiticus in yeast extract sucrose medium |
| url | http://dx.doi.org/10.1155/2009/842626 |
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