The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis
Abstract Objective Saliva, which is a critical component of the oral ecosystem, undergoes dynamic changes, particularly during the onset and progression of periodontitis. Subjects and methods This study used Gas Chromatography-Mass Spectrometry (GC-MS), a reliable and high-throughput tool for metabo...
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BMC
2025-04-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-025-05792-y |
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| author | Lijun Wang Wen Lu Wei Ju Wenxin Yao Chaowen Shi Xihu Yang Wenhao Qian |
| author_facet | Lijun Wang Wen Lu Wei Ju Wenxin Yao Chaowen Shi Xihu Yang Wenhao Qian |
| author_sort | Lijun Wang |
| collection | DOAJ |
| description | Abstract Objective Saliva, which is a critical component of the oral ecosystem, undergoes dynamic changes, particularly during the onset and progression of periodontitis. Subjects and methods This study used Gas Chromatography-Mass Spectrometry (GC-MS), a reliable and high-throughput tool for metabolomic analysis, to detect salivary metabolic shifts across various stages of periodontitis (T1-T4). We compared differential changes in metabolites between the HC and T1 groups, the T1 and T2 groups, the T2 and T3 groups, and the T3 and T4 groups. Results By analysing saliva samples from 116 individuals-10 healthy controls (HC) and 106 patients with periodontitis across stages T1 (22 individuals) to T4 (28 individuals), we identified differential metabolites including Glucose, 3-Aminobutanoic Acid, N-(1-Cyclopropylethyl) Aniline, and Methylmalonic Acid. Compared to HC, the metabolites in patients with periodontitis exhibited progressive concentration changes correlating with the severity of the disease. Furthermore, KEGG pathway analysis was used to elucidate the metabolic pathways involved in the development of periodontitis. Conclusions Our findings demonstrate the potential of salivary metabolites as biomarkers for monitoring periodontitis progression and offer valuable insights into its pathogenesis and potential therapeutic targets. |
| format | Article |
| id | doaj-art-b36efce9cdea40a28e3de00313d3403c |
| institution | OA Journals |
| issn | 1472-6831 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj-art-b36efce9cdea40a28e3de00313d3403c2025-08-20T02:08:11ZengBMCBMC Oral Health1472-68312025-04-012511910.1186/s12903-025-05792-yThe salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitisLijun Wang0Wen Lu1Wei Ju2Wenxin Yao3Chaowen Shi4Xihu Yang5Wenhao Qian6Department of Periodontitis, Xuhui District Dental CenterDepartment of Oral and Maxillofacial Surgery, Affiliated Hospital of Jiangsu UniversityDepartment of Oral and Maxillofacial Surgery, Affiliated Hospital of Jiangsu UniversityDepartment of Periodontitis, Xuhui District Dental CenterSchool of Life Sciences, Jiangsu UniversityDepartment of Oral and Maxillofacial Surgery, Affiliated Hospital of Jiangsu UniversityDepartment of Periodontitis, Xuhui District Dental CenterAbstract Objective Saliva, which is a critical component of the oral ecosystem, undergoes dynamic changes, particularly during the onset and progression of periodontitis. Subjects and methods This study used Gas Chromatography-Mass Spectrometry (GC-MS), a reliable and high-throughput tool for metabolomic analysis, to detect salivary metabolic shifts across various stages of periodontitis (T1-T4). We compared differential changes in metabolites between the HC and T1 groups, the T1 and T2 groups, the T2 and T3 groups, and the T3 and T4 groups. Results By analysing saliva samples from 116 individuals-10 healthy controls (HC) and 106 patients with periodontitis across stages T1 (22 individuals) to T4 (28 individuals), we identified differential metabolites including Glucose, 3-Aminobutanoic Acid, N-(1-Cyclopropylethyl) Aniline, and Methylmalonic Acid. Compared to HC, the metabolites in patients with periodontitis exhibited progressive concentration changes correlating with the severity of the disease. Furthermore, KEGG pathway analysis was used to elucidate the metabolic pathways involved in the development of periodontitis. Conclusions Our findings demonstrate the potential of salivary metabolites as biomarkers for monitoring periodontitis progression and offer valuable insights into its pathogenesis and potential therapeutic targets.https://doi.org/10.1186/s12903-025-05792-yGas Chromatography-Mass spectrometry (GC-MS)Salivary metabolomicsPeriodontitis (T1-T4) |
| spellingShingle | Lijun Wang Wen Lu Wei Ju Wenxin Yao Chaowen Shi Xihu Yang Wenhao Qian The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis BMC Oral Health Gas Chromatography-Mass spectrometry (GC-MS) Salivary metabolomics Periodontitis (T1-T4) |
| title | The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis |
| title_full | The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis |
| title_fullStr | The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis |
| title_full_unstemmed | The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis |
| title_short | The salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis |
| title_sort | salivary metabolomics analyses reveal the variable metabolites in distinct staging of periodontitis |
| topic | Gas Chromatography-Mass spectrometry (GC-MS) Salivary metabolomics Periodontitis (T1-T4) |
| url | https://doi.org/10.1186/s12903-025-05792-y |
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