Involvement of different protein kinases and phospholipases A2 in phorbol ester (TPA)-induced arachidonic acid liberation in bovine platelets
The effect of various phospholipase A2 and protein kinase inhibitors on the arachidonic acid liberation in bovine platelets induced by the protein kinase activator 12-O-tetradecanoylphorbol–13-acetate (TPA) was studied. TPA stimulates arachidonic acid release mainly by activating group IV cytosolic...
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| Format: | Article |
| Language: | English |
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Wiley
2000-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1080/09629350050024357 |
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| author | M. Lehr K. Griessbach |
| author_facet | M. Lehr K. Griessbach |
| author_sort | M. Lehr |
| collection | DOAJ |
| description | The effect of various phospholipase A2 and protein kinase inhibitors on the arachidonic acid liberation in bovine platelets induced by the protein kinase activator 12-O-tetradecanoylphorbol–13-acetate (TPA) was studied. TPA stimulates arachidonic acid release mainly by activating group IV cytosolic PLA2 (cPLA2), since inhibitors of this enzyme markedly inhibited arachidonic acid formation. However, group VI Ca2+-independent PLA2 (iPLA2) seems to contribute to the arachidonic acid liberation too, since the relatively specific iPLA2 inhibitor bromoenol lactone (BEL) decreased arachidonic acid generation in part. The pronounced inhibition of the TPA-induced arachidonic acid release by the protein kinase C (PKC) inhibitors GF 109203X and Ro 31–82220, respectively, and by the p38 MAP kinase inhibitor SB 202190 suggests that the activation of the PLA2s by TPA is mediated via PKC and p38 MAP kinase. |
| format | Article |
| id | doaj-art-b33c01234d234e4aa449f8e77ac124c1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2000-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b33c01234d234e4aa449f8e77ac124c12025-02-03T01:32:57ZengWileyMediators of Inflammation0962-93511466-18612000-01-0191313410.1080/09629350050024357Involvement of different protein kinases and phospholipases A2 in phorbol ester (TPA)-induced arachidonic acid liberation in bovine plateletsM. Lehr0K. Griessbach1Institute of Pharmaceutical Chemistry, University of Münster, Hittorfstrasse 58–62, Münster D–48149, GermanyInstitute of Pharmaceutical Chemistry, University of Münster, Hittorfstrasse 58–62, Münster D–48149, GermanyThe effect of various phospholipase A2 and protein kinase inhibitors on the arachidonic acid liberation in bovine platelets induced by the protein kinase activator 12-O-tetradecanoylphorbol–13-acetate (TPA) was studied. TPA stimulates arachidonic acid release mainly by activating group IV cytosolic PLA2 (cPLA2), since inhibitors of this enzyme markedly inhibited arachidonic acid formation. However, group VI Ca2+-independent PLA2 (iPLA2) seems to contribute to the arachidonic acid liberation too, since the relatively specific iPLA2 inhibitor bromoenol lactone (BEL) decreased arachidonic acid generation in part. The pronounced inhibition of the TPA-induced arachidonic acid release by the protein kinase C (PKC) inhibitors GF 109203X and Ro 31–82220, respectively, and by the p38 MAP kinase inhibitor SB 202190 suggests that the activation of the PLA2s by TPA is mediated via PKC and p38 MAP kinase.http://dx.doi.org/10.1080/09629350050024357 |
| spellingShingle | M. Lehr K. Griessbach Involvement of different protein kinases and phospholipases A2 in phorbol ester (TPA)-induced arachidonic acid liberation in bovine platelets Mediators of Inflammation |
| title | Involvement of different protein kinases and phospholipases A2 in phorbol ester (TPA)-induced arachidonic acid liberation in bovine platelets |
| title_full | Involvement of different protein kinases and phospholipases A2 in phorbol ester (TPA)-induced arachidonic acid liberation in bovine platelets |
| title_fullStr | Involvement of different protein kinases and phospholipases A2 in phorbol ester (TPA)-induced arachidonic acid liberation in bovine platelets |
| title_full_unstemmed | Involvement of different protein kinases and phospholipases A2 in phorbol ester (TPA)-induced arachidonic acid liberation in bovine platelets |
| title_short | Involvement of different protein kinases and phospholipases A2 in phorbol ester (TPA)-induced arachidonic acid liberation in bovine platelets |
| title_sort | involvement of different protein kinases and phospholipases a2 in phorbol ester tpa induced arachidonic acid liberation in bovine platelets |
| url | http://dx.doi.org/10.1080/09629350050024357 |
| work_keys_str_mv | AT mlehr involvementofdifferentproteinkinasesandphospholipasesa2inphorbolestertpainducedarachidonicacidliberationinbovineplatelets AT kgriessbach involvementofdifferentproteinkinasesandphospholipasesa2inphorbolestertpainducedarachidonicacidliberationinbovineplatelets |