A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus
The aberrant activity of CD4+ T cells in patients with systemic lupus erythematosus (SLE) is associated with DNA hypomethylation of the regulatory regions in CD11a and CD70 genes. Our previous studies demonstrated that Gadd45a contributes to the development of SLE by promoting DNA demethylation in C...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/206298 |
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| author | Yaping Li Chenghui Huang Ming Zhao Gongping Liang Rong Xiao Susan Yung Tak Mao Chan Qianjin Lu |
| author_facet | Yaping Li Chenghui Huang Ming Zhao Gongping Liang Rong Xiao Susan Yung Tak Mao Chan Qianjin Lu |
| author_sort | Yaping Li |
| collection | DOAJ |
| description | The aberrant activity of CD4+ T cells in patients with systemic lupus erythematosus (SLE) is associated with DNA hypomethylation of the regulatory regions in CD11a and CD70 genes. Our previous studies demonstrated that Gadd45a contributes to the development of SLE by promoting DNA demethylation in CD4+ T cells. In this study, we identified proteins that bind to Gadd45a in CD4+ T cells during SLE flare by using the method of co-immunoprecipitation and mass spectrometry, High mobility group box protein 1 (HMGB1) is one of identified proteins. Furthermore, gene and protein expression of HMGB1 was significantly increased in SLE CD4+ T cells compared to controls, and HMGB1 mRNA was correlated with CD11a and CD70 mRNA. A significant, positive correlation was found between HMGB1 mRNA and SLEDAI for SLE patients. Our data demonstrate that HMGB1 binds to Gadd45a and may be involved in DNA demethylation in CD4+ T cells during lupus flare. |
| format | Article |
| id | doaj-art-b31b71eb425743c796350ff07b7cb2d1 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-b31b71eb425743c796350ff07b7cb2d12025-02-03T01:29:58ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/206298206298A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus ErythematosusYaping Li0Chenghui Huang1Ming Zhao2Gongping Liang3Rong Xiao4Susan Yung5Tak Mao Chan6Qianjin Lu7Department of Dermatology and Epigenetic Research Center, Key Laboratory of Diabetes Immunology of Ministry of Education, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Oncology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Dermatology and Epigenetic Research Center, Key Laboratory of Diabetes Immunology of Ministry of Education, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Dermatology and Epigenetic Research Center, Key Laboratory of Diabetes Immunology of Ministry of Education, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Dermatology and Epigenetic Research Center, Key Laboratory of Diabetes Immunology of Ministry of Education, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Medicine, The University of Hong Kong, Hong KongDepartment of Medicine, The University of Hong Kong, Hong KongDepartment of Dermatology and Epigenetic Research Center, Key Laboratory of Diabetes Immunology of Ministry of Education, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaThe aberrant activity of CD4+ T cells in patients with systemic lupus erythematosus (SLE) is associated with DNA hypomethylation of the regulatory regions in CD11a and CD70 genes. Our previous studies demonstrated that Gadd45a contributes to the development of SLE by promoting DNA demethylation in CD4+ T cells. In this study, we identified proteins that bind to Gadd45a in CD4+ T cells during SLE flare by using the method of co-immunoprecipitation and mass spectrometry, High mobility group box protein 1 (HMGB1) is one of identified proteins. Furthermore, gene and protein expression of HMGB1 was significantly increased in SLE CD4+ T cells compared to controls, and HMGB1 mRNA was correlated with CD11a and CD70 mRNA. A significant, positive correlation was found between HMGB1 mRNA and SLEDAI for SLE patients. Our data demonstrate that HMGB1 binds to Gadd45a and may be involved in DNA demethylation in CD4+ T cells during lupus flare.http://dx.doi.org/10.1155/2013/206298 |
| spellingShingle | Yaping Li Chenghui Huang Ming Zhao Gongping Liang Rong Xiao Susan Yung Tak Mao Chan Qianjin Lu A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus Clinical and Developmental Immunology |
| title | A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus |
| title_full | A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus |
| title_fullStr | A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus |
| title_full_unstemmed | A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus |
| title_short | A Possible Role of HMGB1 in DNA Demethylation in CD4+ T Cells from Patients with Systemic Lupus Erythematosus |
| title_sort | possible role of hmgb1 in dna demethylation in cd4 t cells from patients with systemic lupus erythematosus |
| url | http://dx.doi.org/10.1155/2013/206298 |
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