INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUM
Intergenomic chromosome pairing was analyzed in unique allohaploid hybrids, which had been produced through a process of somatic hybridization followed by a haploidization procedure (in vitro androgenesis). Two hybrid combinations were studied: (1) between cultivated potato S. tuberosum (dihaploid,...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/293 |
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| author | T. A. Gavrilenko G. I. Pendinen V.-M. Rokka O. Y. Antonova R. Thieme |
| author_facet | T. A. Gavrilenko G. I. Pendinen V.-M. Rokka O. Y. Antonova R. Thieme |
| author_sort | T. A. Gavrilenko |
| collection | DOAJ |
| description | Intergenomic chromosome pairing was analyzed in unique allohaploid hybrids, which had been produced through a process of somatic hybridization followed by a haploidization procedure (in vitro androgenesis). Two hybrid combinations were studied: (1) between cultivated potato S. tuberosum (dihaploid, 2n = 2x = 24, AA genome) and wild species S. etuberosum (2n = 2x = 24, EE genome), (2) between cultivated tomato S. lycopersicum (LL, 2n = 2x = 24) and wild species S. etuberosum (2n = 2x = 24, EE). Genome and chromosome composition of allohaploids were identified through FISH with chromosome specific BAC clones and GISH. Up to 7 intergenomic bivalents per cell were observed in allohaploids between S. tuberosum and S. etuberosum (AE); the chiasmata were distributed in the distal regions of long arms of each chromosome and in the short arms of chromosomes 3, 6, 11, 12. Rare bivalents (from 0 to 2) were observed in allohaploids between S. lycopersicum and S. etuberosum (LE); the chiasmata were distributed in the long arms of chromosome 4 and 6. The perspectives of proposed approach are discussed for application of somatic hybridization and chromosome engineering to study the potential of homeologous pairing and the strategy of introgression. |
| format | Article |
| id | doaj-art-b2e3806dfd3642bd83847dd1653954a6 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-b2e3806dfd3642bd83847dd1653954a62025-02-01T09:58:01ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01184/1650659277INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUMT. A. Gavrilenko0G. I. Pendinen1V.-M. Rokka2O. Y. Antonova3R. Thieme4Vavilov Institute of Plant Industry, Saint-Petersburg, Russia Saint-Petersburg State University, Saint-Petersburg, RussiaVavilov Institute of Plant Industry, Saint-Petersburg, RussiaMTT Agrifood Research Finland, Biotechnology and Food Research, Jokioinen, FinlandVavilov Institute of Plant Industry, Saint-Petersburg, RussiaJulius Kühn-Institut, Federal Research Centre for Cultivated Plants, Quedlinburg, GermanyIntergenomic chromosome pairing was analyzed in unique allohaploid hybrids, which had been produced through a process of somatic hybridization followed by a haploidization procedure (in vitro androgenesis). Two hybrid combinations were studied: (1) between cultivated potato S. tuberosum (dihaploid, 2n = 2x = 24, AA genome) and wild species S. etuberosum (2n = 2x = 24, EE genome), (2) between cultivated tomato S. lycopersicum (LL, 2n = 2x = 24) and wild species S. etuberosum (2n = 2x = 24, EE). Genome and chromosome composition of allohaploids were identified through FISH with chromosome specific BAC clones and GISH. Up to 7 intergenomic bivalents per cell were observed in allohaploids between S. tuberosum and S. etuberosum (AE); the chiasmata were distributed in the distal regions of long arms of each chromosome and in the short arms of chromosomes 3, 6, 11, 12. Rare bivalents (from 0 to 2) were observed in allohaploids between S. lycopersicum and S. etuberosum (LE); the chiasmata were distributed in the long arms of chromosome 4 and 6. The perspectives of proposed approach are discussed for application of somatic hybridization and chromosome engineering to study the potential of homeologous pairing and the strategy of introgression.https://vavilov.elpub.ru/jour/article/view/293solanumpotatotomatoetuberosuminterspecific hybridizationhomeologous chromosome pairingfishgish |
| spellingShingle | T. A. Gavrilenko G. I. Pendinen V.-M. Rokka O. Y. Antonova R. Thieme INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUM Вавиловский журнал генетики и селекции solanum potato tomato etuberosum interspecific hybridization homeologous chromosome pairing fish gish |
| title | INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUM |
| title_full | INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUM |
| title_fullStr | INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUM |
| title_full_unstemmed | INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUM |
| title_short | INTERGENOMIC CHROMOSOME PAIRING IN ALLOHAPLOID HYBRIDS OF GENUS SOLANUM |
| title_sort | intergenomic chromosome pairing in allohaploid hybrids of genus solanum |
| topic | solanum potato tomato etuberosum interspecific hybridization homeologous chromosome pairing fish gish |
| url | https://vavilov.elpub.ru/jour/article/view/293 |
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