Nematode controlling effects and safety tests of Duddingtonia flagrans biological preparation in sheep
Abstract Duddingtonia flagrans is a nematode-trapping fungus that is widely used to control parasitic nematodes in livestock. After oral ingestion and passage through the digestive tract of animals, this microorganism captures nematodes in feces. Although many researchers have examined the safety of...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-85844-z |
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| author | Yuan Ma Lili Jiang Zhaobin Fan Luyao Hao Zhengyi Li Yanni Zhang Qiannan Li Rui Wang Hongliang Luo |
| author_facet | Yuan Ma Lili Jiang Zhaobin Fan Luyao Hao Zhengyi Li Yanni Zhang Qiannan Li Rui Wang Hongliang Luo |
| author_sort | Yuan Ma |
| collection | DOAJ |
| description | Abstract Duddingtonia flagrans is a nematode-trapping fungus that is widely used to control parasitic nematodes in livestock. After oral ingestion and passage through the digestive tract of animals, this microorganism captures nematodes in feces. Although many researchers have examined the safety of this fungus for humans, animals, and the environment, few reports have discussed the safety of nematode-trapping D. flagrans biologics for animals. In this study, D. flagrans safety was tested, while adverse effects and toxicities were examined in sheep. First, the nematode killing effects in naturally parasitized sheep after administration of lyophilized D. flagrans preparations were tested.lyophilized D. flagrans preparations were administered to sheep at various doses, followed by key blood factor monitoring and an examination of major tissues, organ lesions, and pathology. Lastly, lyophilized D. flagrans preparations were administered to sheep at various doses, followed by key blood factor monitoring and an examination of major tissues, organ lesions, and pathology. the nematode killing effects of naturally parasitized sheep after administration were tested. The results demonstrated that treatment with D. flagrans isolates significantly reduced developing larvae numbers in feces, with an efficiency of 92.99%. Lyophilized preparations had no observable effects on physiological parameters in sheep, thus indicating a wide safety range in target animals, with potentially minimal risks in veterinary clinical practice. Overall, D. flagrans freeze-dried biologics effectively helped to controlled parasitic infections, which are safe in animals like sheep, and thus may provide a practical platform for nematode-trapping fungi in veterinary clinical settings. |
| format | Article |
| id | doaj-art-b2e0d4715dbd43c8ac91bb2700ff9552 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-b2e0d4715dbd43c8ac91bb2700ff95522025-01-19T12:24:08ZengNature PortfolioScientific Reports2045-23222025-01-011511810.1038/s41598-025-85844-zNematode controlling effects and safety tests of Duddingtonia flagrans biological preparation in sheepYuan Ma0Lili Jiang1Zhaobin Fan2Luyao Hao3Zhengyi Li4Yanni Zhang5Qiannan Li6Rui Wang7Hongliang Luo8College of Veterinary Medicine, Inner Mongolia Agricultural UniversityCollege of Pharmacy Heze UniversityCollege of Pharmacy Heze UniversityCollege of Veterinary Medicine, Inner Mongolia Agricultural UniversityCollege of Veterinary Medicine, Inner Mongolia Agricultural UniversityCollege of Veterinary Medicine, Inner Mongolia Agricultural UniversityCollege of Veterinary Medicine, Inner Mongolia Agricultural UniversityCollege of Veterinary Medicine, Inner Mongolia Agricultural UniversityRui Pu Agricultural Technology Co., LtdAbstract Duddingtonia flagrans is a nematode-trapping fungus that is widely used to control parasitic nematodes in livestock. After oral ingestion and passage through the digestive tract of animals, this microorganism captures nematodes in feces. Although many researchers have examined the safety of this fungus for humans, animals, and the environment, few reports have discussed the safety of nematode-trapping D. flagrans biologics for animals. In this study, D. flagrans safety was tested, while adverse effects and toxicities were examined in sheep. First, the nematode killing effects in naturally parasitized sheep after administration of lyophilized D. flagrans preparations were tested.lyophilized D. flagrans preparations were administered to sheep at various doses, followed by key blood factor monitoring and an examination of major tissues, organ lesions, and pathology. Lastly, lyophilized D. flagrans preparations were administered to sheep at various doses, followed by key blood factor monitoring and an examination of major tissues, organ lesions, and pathology. the nematode killing effects of naturally parasitized sheep after administration were tested. The results demonstrated that treatment with D. flagrans isolates significantly reduced developing larvae numbers in feces, with an efficiency of 92.99%. Lyophilized preparations had no observable effects on physiological parameters in sheep, thus indicating a wide safety range in target animals, with potentially minimal risks in veterinary clinical practice. Overall, D. flagrans freeze-dried biologics effectively helped to controlled parasitic infections, which are safe in animals like sheep, and thus may provide a practical platform for nematode-trapping fungi in veterinary clinical settings.https://doi.org/10.1038/s41598-025-85844-zLyophilized biologicSafety profilesKilling effectsNematode-trapping fungi |
| spellingShingle | Yuan Ma Lili Jiang Zhaobin Fan Luyao Hao Zhengyi Li Yanni Zhang Qiannan Li Rui Wang Hongliang Luo Nematode controlling effects and safety tests of Duddingtonia flagrans biological preparation in sheep Scientific Reports Lyophilized biologic Safety profiles Killing effects Nematode-trapping fungi |
| title | Nematode controlling effects and safety tests of Duddingtonia flagrans biological preparation in sheep |
| title_full | Nematode controlling effects and safety tests of Duddingtonia flagrans biological preparation in sheep |
| title_fullStr | Nematode controlling effects and safety tests of Duddingtonia flagrans biological preparation in sheep |
| title_full_unstemmed | Nematode controlling effects and safety tests of Duddingtonia flagrans biological preparation in sheep |
| title_short | Nematode controlling effects and safety tests of Duddingtonia flagrans biological preparation in sheep |
| title_sort | nematode controlling effects and safety tests of duddingtonia flagrans biological preparation in sheep |
| topic | Lyophilized biologic Safety profiles Killing effects Nematode-trapping fungi |
| url | https://doi.org/10.1038/s41598-025-85844-z |
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