Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilage
In contrast to decellularization of soft tissues for use as tissue-specific matrices in the creation of tissue-engineered constructs, decellularization of cartilage tissue requires several processing techniques, which can negatively affect the biocompatibility and functional properties of the native...
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Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
2022-12-01
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| Series: | Вестник трансплантологии и искусственных органов |
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| Online Access: | https://journal.transpl.ru/vtio/article/view/1553 |
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| author | E. A. Nemets A. E. Lazhko A. M. Grigoriev Yu. B. Basok A. D. Kirillova V. I. Sevastianov |
| author_facet | E. A. Nemets A. E. Lazhko A. M. Grigoriev Yu. B. Basok A. D. Kirillova V. I. Sevastianov |
| author_sort | E. A. Nemets |
| collection | DOAJ |
| description | In contrast to decellularization of soft tissues for use as tissue-specific matrices in the creation of tissue-engineered constructs, decellularization of cartilage tissue requires several processing techniques, which can negatively affect the biocompatibility and functional properties of the native extracellular matrix (ECM).Objective: to study the biocompatible and functional properties of microdispersed tissue-specific 3D matrix from a porcine cartilage that is decellularized by sequential use of chemical, physical and enzymatic techniques.Materials and methods. For decellularization, microdispersed cartilage particles (MCPs), obtained by cryomilling, were incubated in detergent solutions (sodium dodecyl sulfate and Triton X-100), then treated with supercritical carbon dioxide (scCO2) with 10% ethanol and DNase I. The Ames test (Salmonella typhimurium reverse mutation assay) was used to determine the genotoxicity of decellularized microdispersed cartilage particles (dMCPs). Local and general toxic effects, as well as resorption of dMCPs were studied in vivo on sexually mature outbred rats. Decellularized MCP specimens (10 mg) were implanted into the thigh muscle tissue. Viability of human adipose-derived mesenchymal stem/stromal cells (hAdMSCs), when cultured on dMCPs, was analyzed by in vivo microscopy, stained with fluorescent Calcein AM dye. Cell metabolic activity was assessed using PrestoBlue™ Cell Viability Reagent.Results. It has been proven that porcine dMCPs implanted in rat muscle after treatment with scCO2 do not exhibit local and general toxic effects, and do not show genotoxicity and negative effects on the reproductive system of animals. After 6 months of in vivo experiment, most (87%) of the implanted decellularized cartilage was resorbed. It was shown that the resulting matrices are able to support adhesion and proliferation of hAdMSCs. Conclusion. Porcine dMCP specimens are suitable for biocompatible medical products in terms of local and general toxic effects, genotoxicity and reproductive toxicity, and can be used as a matrix for creating cell- and tissue-engineered cartilage constructs. |
| format | Article |
| id | doaj-art-b2c83101bbd1467fa7985dff80bb072f |
| institution | Kabale University |
| issn | 1995-1191 |
| language | Russian |
| publishDate | 2022-12-01 |
| publisher | Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov |
| record_format | Article |
| series | Вестник трансплантологии и искусственных органов |
| spelling | doaj-art-b2c83101bbd1467fa7985dff80bb072f2025-08-20T03:44:23ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.ShumakovВестник трансплантологии и искусственных органов1995-11912022-12-01244738410.15825/1995-1191-2022-4-73-841130Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilageE. A. Nemets0A. E. Lazhko1A. M. Grigoriev2Yu. B. Basok3A. D. Kirillova4V. I. Sevastianov5Shumakov National Medical Research Center of Transplantology and Artificial OrgansMoscow State UniversityShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial OrgansShumakov National Medical Research Center of Transplantology and Artificial Organs; Institute of Biomedical Research and TechnologyIn contrast to decellularization of soft tissues for use as tissue-specific matrices in the creation of tissue-engineered constructs, decellularization of cartilage tissue requires several processing techniques, which can negatively affect the biocompatibility and functional properties of the native extracellular matrix (ECM).Objective: to study the biocompatible and functional properties of microdispersed tissue-specific 3D matrix from a porcine cartilage that is decellularized by sequential use of chemical, physical and enzymatic techniques.Materials and methods. For decellularization, microdispersed cartilage particles (MCPs), obtained by cryomilling, were incubated in detergent solutions (sodium dodecyl sulfate and Triton X-100), then treated with supercritical carbon dioxide (scCO2) with 10% ethanol and DNase I. The Ames test (Salmonella typhimurium reverse mutation assay) was used to determine the genotoxicity of decellularized microdispersed cartilage particles (dMCPs). Local and general toxic effects, as well as resorption of dMCPs were studied in vivo on sexually mature outbred rats. Decellularized MCP specimens (10 mg) were implanted into the thigh muscle tissue. Viability of human adipose-derived mesenchymal stem/stromal cells (hAdMSCs), when cultured on dMCPs, was analyzed by in vivo microscopy, stained with fluorescent Calcein AM dye. Cell metabolic activity was assessed using PrestoBlue™ Cell Viability Reagent.Results. It has been proven that porcine dMCPs implanted in rat muscle after treatment with scCO2 do not exhibit local and general toxic effects, and do not show genotoxicity and negative effects on the reproductive system of animals. After 6 months of in vivo experiment, most (87%) of the implanted decellularized cartilage was resorbed. It was shown that the resulting matrices are able to support adhesion and proliferation of hAdMSCs. Conclusion. Porcine dMCP specimens are suitable for biocompatible medical products in terms of local and general toxic effects, genotoxicity and reproductive toxicity, and can be used as a matrix for creating cell- and tissue-engineered cartilage constructs.https://journal.transpl.ru/vtio/article/view/1553articular cartilagedecellularization3d matrixresorptionbiocompatibilityadipose-derived mscsadhesionproliferationtissue-engineered construct |
| spellingShingle | E. A. Nemets A. E. Lazhko A. M. Grigoriev Yu. B. Basok A. D. Kirillova V. I. Sevastianov Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilage Вестник трансплантологии и искусственных органов articular cartilage decellularization 3d matrix resorption biocompatibility adipose-derived mscs adhesion proliferation tissue-engineered construct |
| title | Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilage |
| title_full | Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilage |
| title_fullStr | Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilage |
| title_full_unstemmed | Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilage |
| title_short | Biocompatible and functional properties of a microdispersed tissue-specific 3D matrix from decellularized porcine cartilage |
| title_sort | biocompatible and functional properties of a microdispersed tissue specific 3d matrix from decellularized porcine cartilage |
| topic | articular cartilage decellularization 3d matrix resorption biocompatibility adipose-derived mscs adhesion proliferation tissue-engineered construct |
| url | https://journal.transpl.ru/vtio/article/view/1553 |
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