Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115
Probiotic bacteria improve human health by secreting pro-microbial substances, balancing intestinal flora, binding to the mucous membrane and epithelium, strengthening the intestinal epithelial barrier, and creating interactions between the gastrointestinal microbiota and the immune system. This stu...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | Biofilm |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207525000036 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832591789909868544 |
|---|---|
| author | Behrooz Alizadeh Behbahani Mostafa Rahmati-Joneidabad Morteza Taki |
| author_facet | Behrooz Alizadeh Behbahani Mostafa Rahmati-Joneidabad Morteza Taki |
| author_sort | Behrooz Alizadeh Behbahani |
| collection | DOAJ |
| description | Probiotic bacteria improve human health by secreting pro-microbial substances, balancing intestinal flora, binding to the mucous membrane and epithelium, strengthening the intestinal epithelial barrier, and creating interactions between the gastrointestinal microbiota and the immune system. This study aimed to investigate the probiotic potential, biofilm-related gene expression and anti-biofilm capabilities of Lactiplantibacillus pentosus 6MMI. The strain exhibited remarkable resilience to challenging conditions, including acidic environments, gastrointestinal settings, and bile salts. Notably, Lpb. pentosus demonstrated significant hydrophobicity (71.89 %), auto-aggregation (42.39 %), co-aggregation (51.28 %), antioxidant activity (ranging from 42.29 % to 64.61 %), and a cholesterol reduction capacity of 50.31 %. Its competitive abilities against Listeria monocytogenes were quantified, showing a competition rate of 54.51 %, displacement rate of 48.57 %, and inhibition of adhesion at 27.71 %. Also, Lpb. pentosus resulted an adhesion rate of 12.91 % to epithelial cells and showed no DNase or hemolytic activity. The strain exhibited the highest resistance to nalidixic acid, with an inhibition zone measuring 15.20 mm, while it was least resistant to chloramphenicol, which had an inhibition zone of 27.30 mm. Treatment with cell-free supernatant (CFS) from Lpb. pentosus significantly reduced biofilm formation by 91.25 % and 24.50 % and diminished mature biofilm formation by 83.82 % and 21.80 % on L. monocytogenes. Additionally, the CFS inhibited the transcription of the plcB, hly, and prfA genes in L. monocytogenes, suggesting a potential reduction in bacterial virulence through decreased hemolysin release and modulation of phospholipase activity. In the next step of the study, the Gaussian Process Regression (GPR) model accurately predicted bile tolerance and acid parameters with a high R2 of 0.99 and minimal Mean Absolute Percentage Error (MAPE) values of 0.33 % and 0.21 %, respectively. The residual errors showed a normal distribution, indicating reliable and consistent predictions. Overall, Lpb. pentosus 6MMI represents a valuable candidate for further investigation in probiotic development and biofilm management strategies. |
| format | Article |
| id | doaj-art-b2b9173a0541444cbb44b55b4bc56351 |
| institution | Kabale University |
| issn | 2590-2075 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj-art-b2b9173a0541444cbb44b55b4bc563512025-01-22T05:43:59ZengElsevierBiofilm2590-20752025-06-019100255Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115Behrooz Alizadeh Behbahani0Mostafa Rahmati-Joneidabad1Morteza Taki2Department of Food Science and Technology, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran; Corresponding author.Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, IranDepartment of Agricultural Machinery and Mechanization Engineering, Faculty of Agricultural Engineering and Rural Development, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, IranProbiotic bacteria improve human health by secreting pro-microbial substances, balancing intestinal flora, binding to the mucous membrane and epithelium, strengthening the intestinal epithelial barrier, and creating interactions between the gastrointestinal microbiota and the immune system. This study aimed to investigate the probiotic potential, biofilm-related gene expression and anti-biofilm capabilities of Lactiplantibacillus pentosus 6MMI. The strain exhibited remarkable resilience to challenging conditions, including acidic environments, gastrointestinal settings, and bile salts. Notably, Lpb. pentosus demonstrated significant hydrophobicity (71.89 %), auto-aggregation (42.39 %), co-aggregation (51.28 %), antioxidant activity (ranging from 42.29 % to 64.61 %), and a cholesterol reduction capacity of 50.31 %. Its competitive abilities against Listeria monocytogenes were quantified, showing a competition rate of 54.51 %, displacement rate of 48.57 %, and inhibition of adhesion at 27.71 %. Also, Lpb. pentosus resulted an adhesion rate of 12.91 % to epithelial cells and showed no DNase or hemolytic activity. The strain exhibited the highest resistance to nalidixic acid, with an inhibition zone measuring 15.20 mm, while it was least resistant to chloramphenicol, which had an inhibition zone of 27.30 mm. Treatment with cell-free supernatant (CFS) from Lpb. pentosus significantly reduced biofilm formation by 91.25 % and 24.50 % and diminished mature biofilm formation by 83.82 % and 21.80 % on L. monocytogenes. Additionally, the CFS inhibited the transcription of the plcB, hly, and prfA genes in L. monocytogenes, suggesting a potential reduction in bacterial virulence through decreased hemolysin release and modulation of phospholipase activity. In the next step of the study, the Gaussian Process Regression (GPR) model accurately predicted bile tolerance and acid parameters with a high R2 of 0.99 and minimal Mean Absolute Percentage Error (MAPE) values of 0.33 % and 0.21 %, respectively. The residual errors showed a normal distribution, indicating reliable and consistent predictions. Overall, Lpb. pentosus 6MMI represents a valuable candidate for further investigation in probiotic development and biofilm management strategies.http://www.sciencedirect.com/science/article/pii/S2590207525000036Anti-biofilmGene expressionModelingProbiotic |
| spellingShingle | Behrooz Alizadeh Behbahani Mostafa Rahmati-Joneidabad Morteza Taki Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115 Biofilm Anti-biofilm Gene expression Modeling Probiotic |
| title | Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115 |
| title_full | Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115 |
| title_fullStr | Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115 |
| title_full_unstemmed | Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115 |
| title_short | Examining the impact of probiotic Lactiplantibacillus pentosus 6MMI on inhibiting biofilm formation, adhesion, and virulence gene expression in Listeria monocytogenes ATCC 19115 |
| title_sort | examining the impact of probiotic lactiplantibacillus pentosus 6mmi on inhibiting biofilm formation adhesion and virulence gene expression in listeria monocytogenes atcc 19115 |
| topic | Anti-biofilm Gene expression Modeling Probiotic |
| url | http://www.sciencedirect.com/science/article/pii/S2590207525000036 |
| work_keys_str_mv | AT behroozalizadehbehbahani examiningtheimpactofprobioticlactiplantibacilluspentosus6mmioninhibitingbiofilmformationadhesionandvirulencegeneexpressioninlisteriamonocytogenesatcc19115 AT mostafarahmatijoneidabad examiningtheimpactofprobioticlactiplantibacilluspentosus6mmioninhibitingbiofilmformationadhesionandvirulencegeneexpressioninlisteriamonocytogenesatcc19115 AT mortezataki examiningtheimpactofprobioticlactiplantibacilluspentosus6mmioninhibitingbiofilmformationadhesionandvirulencegeneexpressioninlisteriamonocytogenesatcc19115 |