Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepiali
More and more studies have reported the role of Paecilomyces hepiali polysaccharides in bidirectional regulation (immune enhancement and anti-inflammatory). However, there are few collaborative studies on the structure, immune enhancement, and anti-inflammatory effects of polysaccharides from the fe...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co. Ltd.
2025-11-01
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| Series: | Journal of Future Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772566924000971 |
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| author | Baoming Tian Renjian Liu Qi Li Hanshan Xu Ming Cai Rongfa Guan Peilong Sun Kai Yang |
| author_facet | Baoming Tian Renjian Liu Qi Li Hanshan Xu Ming Cai Rongfa Guan Peilong Sun Kai Yang |
| author_sort | Baoming Tian |
| collection | DOAJ |
| description | More and more studies have reported the role of Paecilomyces hepiali polysaccharides in bidirectional regulation (immune enhancement and anti-inflammatory). However, there are few collaborative studies on the structure, immune enhancement, and anti-inflammatory effects of polysaccharides from the fermentation broth of P. hepiali (PHP) and the mechanisms are still unclear. The present study aimed to clarify the mechanism underlying the dual-immunomodulatory (immune enhancement and anti-inflammatory). PHP40 was precipitated with 40% ethanol from PHP40 and further purified to obtain PHP40-2. The chemical characteristics of PHP40-2 was analyzed by chemical and instrumental analyses, and the double immunomodulatory effects of RAW264.7 macrophages were investigated. PHP40-2 was a heteropolysaccharide composed of →4,6)-α-D-Glcp-(1→, →6)-α-D-Manp-(1→, α-D-Glcp-(1→, and →4)-β-D-Galp-(1→, and its molecular weight was 6.2 × 105 Da. The nitric oxide (NO) production, phagocytosis activity, and cytokine secretion were significantly increased (P < 0.05) after PHP40-2 treatment; however, PHP40-2 could inhibit NO production and cytokine secretion in LPS-induced RAW264.7 macrophages (P < 0.05). After mRNA and protein expression analysis, toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway was discovered to play an important role in the dual-immunomodulatory effects of PHP40-2. This study reveals that the fermentation broth of PHP is also a valuable resource and PHP40-2 has the potential to be developed as a novel functional food. |
| format | Article |
| id | doaj-art-b29373bca8d54643a14b2d6e5d660e16 |
| institution | Kabale University |
| issn | 2772-5669 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Journal of Future Foods |
| spelling | doaj-art-b29373bca8d54643a14b2d6e5d660e162025-01-30T05:15:15ZengKeAi Communications Co. Ltd.Journal of Future Foods2772-56692025-11-0156628640Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepialiBaoming Tian0Renjian Liu1Qi Li2Hanshan Xu3Ming Cai4Rongfa Guan5Peilong Sun6Kai Yang7College of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, Huzhou 313299, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, ChinaHangzhou Xueyu Biotechnology Co., Ltd., Hangzhou 311254, ChinaLinping District of Supervising Testing Center for Quality and Meterology, Hangzhou 311199, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, Huzhou 313299, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, Huzhou 313299, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, Huzhou 313299, China; Corresponding authors at: College of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, China.College of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, Huzhou 313299, China; Corresponding authors at: College of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, China.More and more studies have reported the role of Paecilomyces hepiali polysaccharides in bidirectional regulation (immune enhancement and anti-inflammatory). However, there are few collaborative studies on the structure, immune enhancement, and anti-inflammatory effects of polysaccharides from the fermentation broth of P. hepiali (PHP) and the mechanisms are still unclear. The present study aimed to clarify the mechanism underlying the dual-immunomodulatory (immune enhancement and anti-inflammatory). PHP40 was precipitated with 40% ethanol from PHP40 and further purified to obtain PHP40-2. The chemical characteristics of PHP40-2 was analyzed by chemical and instrumental analyses, and the double immunomodulatory effects of RAW264.7 macrophages were investigated. PHP40-2 was a heteropolysaccharide composed of →4,6)-α-D-Glcp-(1→, →6)-α-D-Manp-(1→, α-D-Glcp-(1→, and →4)-β-D-Galp-(1→, and its molecular weight was 6.2 × 105 Da. The nitric oxide (NO) production, phagocytosis activity, and cytokine secretion were significantly increased (P < 0.05) after PHP40-2 treatment; however, PHP40-2 could inhibit NO production and cytokine secretion in LPS-induced RAW264.7 macrophages (P < 0.05). After mRNA and protein expression analysis, toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway was discovered to play an important role in the dual-immunomodulatory effects of PHP40-2. This study reveals that the fermentation broth of PHP is also a valuable resource and PHP40-2 has the potential to be developed as a novel functional food.http://www.sciencedirect.com/science/article/pii/S2772566924000971Dual-immunomodulatoryPaecilomyces hepiali polysaccharidesFermentation brothRAW264.7 macrophagesStructure of polysaccharides |
| spellingShingle | Baoming Tian Renjian Liu Qi Li Hanshan Xu Ming Cai Rongfa Guan Peilong Sun Kai Yang Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepiali Journal of Future Foods Dual-immunomodulatory Paecilomyces hepiali polysaccharides Fermentation broth RAW264.7 macrophages Structure of polysaccharides |
| title | Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepiali |
| title_full | Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepiali |
| title_fullStr | Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepiali |
| title_full_unstemmed | Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepiali |
| title_short | Dual-immunomodulatory effects on RAW264.7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of Paecilomyces hepiali |
| title_sort | dual immunomodulatory effects on raw264 7 macrophages and structural elucidation of a polysaccharide isolated from fermentation broth of paecilomyces hepiali |
| topic | Dual-immunomodulatory Paecilomyces hepiali polysaccharides Fermentation broth RAW264.7 macrophages Structure of polysaccharides |
| url | http://www.sciencedirect.com/science/article/pii/S2772566924000971 |
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