Evaluation of Tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cells
Breast cancer remains a major public health challenge, highlighting the importance of finding new therapeutic approaches, including the use of traditional plants with unstudied medicinal properties. This study aims to explore the bioactive potential of Tinospora cordifolia stem extract by investigat...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2025-01-01
|
| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2025/05/bioconf_icgrc2025_03010.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832539706314719232 |
|---|---|
| author | Annisa Yuslinda Rosyadah Nuraini Kamila Fairuz Sarah Ulfa Siti Mariyah Lestari Sri Rahayu Widodo Nashi |
| author_facet | Annisa Yuslinda Rosyadah Nuraini Kamila Fairuz Sarah Ulfa Siti Mariyah Lestari Sri Rahayu Widodo Nashi |
| author_sort | Annisa Yuslinda |
| collection | DOAJ |
| description | Breast cancer remains a major public health challenge, highlighting the importance of finding new therapeutic approaches, including the use of traditional plants with unstudied medicinal properties. This study aims to explore the bioactive potential of Tinospora cordifolia stem extract by investigating the content of flavonoids, phenols, terpenoids and alkaloids as well as the cytotoxic and apoptotic effects on T47D breast cancer cells. Analysis of bioactive compound content revealed a total flavonoid content of 29.7 ± 0.30 mg QE/g extract, a total phenol content of 120.4 ± 4.25 mg GAE/g extract, a total terpenoid content of 2.41 ± 2.18 mg LE/g extract and a total alkaloid content of 2.55 ± 0.27 mg LE/g extract. Cytotoxicity tests on T47D cells using the WST-1 assay showed a dosedependent decrease in cell viability with an IC50 of 571.3 ± 33.41 µg/ml. Furthermore, at a concentration of 2IC50, T. cordifolia stem extract can induce apoptosis by 36.7 ± 4.19%. These results suggest that T. cordifolia has considerable anticancer activity, although further studies are needed to understand its mechanism of action and evaluate its potential in breast cancer therapy. |
| format | Article |
| id | doaj-art-b25314cc38b4477f9d0f41a23aef9fbf |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-b25314cc38b4477f9d0f41a23aef9fbf2025-02-05T10:43:07ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011540301010.1051/bioconf/202515403010bioconf_icgrc2025_03010Evaluation of Tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cellsAnnisa Yuslinda0Rosyadah Nuraini1Kamila Fairuz Sarah2Ulfa Siti Mariyah3Lestari Sri Rahayu4Widodo Nashi5Department of Biology, Faculty Mathematics and Natural Science, Universitas BrawijayaDepartment of Biology, Faculty Mathematics and Natural Science, Universitas BrawijayaDepartment of Biology, Faculty Mathematics and Natural Science, Universitas BrawijayaDepartment of Chemistry, Faculty Mathematics and Natural Science, Universitas BrawijayaDepartment of Biology, Faculty Mathematics and Natural Science, Universitas Negeri MalangDepartment of Biology, Faculty Mathematics and Natural Science, Universitas BrawijayaBreast cancer remains a major public health challenge, highlighting the importance of finding new therapeutic approaches, including the use of traditional plants with unstudied medicinal properties. This study aims to explore the bioactive potential of Tinospora cordifolia stem extract by investigating the content of flavonoids, phenols, terpenoids and alkaloids as well as the cytotoxic and apoptotic effects on T47D breast cancer cells. Analysis of bioactive compound content revealed a total flavonoid content of 29.7 ± 0.30 mg QE/g extract, a total phenol content of 120.4 ± 4.25 mg GAE/g extract, a total terpenoid content of 2.41 ± 2.18 mg LE/g extract and a total alkaloid content of 2.55 ± 0.27 mg LE/g extract. Cytotoxicity tests on T47D cells using the WST-1 assay showed a dosedependent decrease in cell viability with an IC50 of 571.3 ± 33.41 µg/ml. Furthermore, at a concentration of 2IC50, T. cordifolia stem extract can induce apoptosis by 36.7 ± 4.19%. These results suggest that T. cordifolia has considerable anticancer activity, although further studies are needed to understand its mechanism of action and evaluate its potential in breast cancer therapy.https://www.bio-conferences.org/articles/bioconf/pdf/2025/05/bioconf_icgrc2025_03010.pdf |
| spellingShingle | Annisa Yuslinda Rosyadah Nuraini Kamila Fairuz Sarah Ulfa Siti Mariyah Lestari Sri Rahayu Widodo Nashi Evaluation of Tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cells BIO Web of Conferences |
| title | Evaluation of Tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cells |
| title_full | Evaluation of Tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cells |
| title_fullStr | Evaluation of Tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cells |
| title_full_unstemmed | Evaluation of Tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cells |
| title_short | Evaluation of Tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cells |
| title_sort | evaluation of tinospora cordifolia stem extract bioactive content and anticancer activity against breast cancer cells |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2025/05/bioconf_icgrc2025_03010.pdf |
| work_keys_str_mv | AT annisayuslinda evaluationoftinosporacordifoliastemextractbioactivecontentandanticanceractivityagainstbreastcancercells AT rosyadahnuraini evaluationoftinosporacordifoliastemextractbioactivecontentandanticanceractivityagainstbreastcancercells AT kamilafairuzsarah evaluationoftinosporacordifoliastemextractbioactivecontentandanticanceractivityagainstbreastcancercells AT ulfasitimariyah evaluationoftinosporacordifoliastemextractbioactivecontentandanticanceractivityagainstbreastcancercells AT lestarisrirahayu evaluationoftinosporacordifoliastemextractbioactivecontentandanticanceractivityagainstbreastcancercells AT widodonashi evaluationoftinosporacordifoliastemextractbioactivecontentandanticanceractivityagainstbreastcancercells |