Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells
Insulin is a cytokine which promotes cell growth. Recently, a few published reports on insulin in different cell lines support the antiapoptotic effect of insulin. But the reports fail to explain the role of insulin in modulating glutamate-mediated neuronal cell death through excitotoxicity. Thus, w...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Behavioural Neurology |
| Online Access: | http://dx.doi.org/10.1155/2014/674164 |
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| author | Madhavan Nampoothiri Neetinkumar D. Reddy Jessy John Nitesh Kumar Gopalan Kutty Nampurath Mallikarjuna Rao Chamallamudi |
| author_facet | Madhavan Nampoothiri Neetinkumar D. Reddy Jessy John Nitesh Kumar Gopalan Kutty Nampurath Mallikarjuna Rao Chamallamudi |
| author_sort | Madhavan Nampoothiri |
| collection | DOAJ |
| description | Insulin is a cytokine which promotes cell growth. Recently, a few published reports on insulin in different cell lines support the antiapoptotic effect of insulin. But the reports fail to explain the role of insulin in modulating glutamate-mediated neuronal cell death through excitotoxicity. Thus, we examined the neuroprotective effect of insulin on glutamate-induced toxicity on differentiated SH-SY5Y neuronal cells. Changes in cell viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) based assay, while apoptotic damage was detected by acridine orange/ethidium bromide and Hoechst staining. Intracellular reactive oxygen species (ROS) accumulation and morphological alterations were also measured. Treatment with glutamate induced apoptosis, elevated ROS levels and caused damage to neurons. Insulin was able to attenuate the glutamate-induced excitotoxic damage to neuronal cells. |
| format | Article |
| id | doaj-art-b232a23723b144b0aa061a38cc72c496 |
| institution | Kabale University |
| issn | 0953-4180 1875-8584 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Behavioural Neurology |
| spelling | doaj-art-b232a23723b144b0aa061a38cc72c4962025-02-03T01:28:12ZengWileyBehavioural Neurology0953-41801875-85842014-01-01201410.1155/2014/674164674164Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal CellsMadhavan Nampoothiri0Neetinkumar D. Reddy1Jessy John2Nitesh Kumar3Gopalan Kutty Nampurath4Mallikarjuna Rao Chamallamudi5Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, IndiaDepartment of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, IndiaDepartment of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, IndiaDepartment of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, IndiaDepartment of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, IndiaDepartment of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal, Karnataka 576104, IndiaInsulin is a cytokine which promotes cell growth. Recently, a few published reports on insulin in different cell lines support the antiapoptotic effect of insulin. But the reports fail to explain the role of insulin in modulating glutamate-mediated neuronal cell death through excitotoxicity. Thus, we examined the neuroprotective effect of insulin on glutamate-induced toxicity on differentiated SH-SY5Y neuronal cells. Changes in cell viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) based assay, while apoptotic damage was detected by acridine orange/ethidium bromide and Hoechst staining. Intracellular reactive oxygen species (ROS) accumulation and morphological alterations were also measured. Treatment with glutamate induced apoptosis, elevated ROS levels and caused damage to neurons. Insulin was able to attenuate the glutamate-induced excitotoxic damage to neuronal cells.http://dx.doi.org/10.1155/2014/674164 |
| spellingShingle | Madhavan Nampoothiri Neetinkumar D. Reddy Jessy John Nitesh Kumar Gopalan Kutty Nampurath Mallikarjuna Rao Chamallamudi Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells Behavioural Neurology |
| title | Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells |
| title_full | Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells |
| title_fullStr | Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells |
| title_full_unstemmed | Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells |
| title_short | Insulin Blocks Glutamate-Induced Neurotoxicity in Differentiated SH-SY5Y Neuronal Cells |
| title_sort | insulin blocks glutamate induced neurotoxicity in differentiated sh sy5y neuronal cells |
| url | http://dx.doi.org/10.1155/2014/674164 |
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