Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice
Curculigoside (CCG) is a phenolic glycoside compound extracted from the root of a natural plant called Curculigo orchioides Gaertn. In this study, the neuroprotective effect of CCG through oxidative stress mediated mitochondrial dysfunction on L-glutamate (L-Glu)-damaged hippocampal neuron cell line...
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002282 |
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| author | Wenqi Wang Yidi Qu Siyu Li Jinyu Chu Hongxin Yang Lirong Teng Di Wang |
| author_facet | Wenqi Wang Yidi Qu Siyu Li Jinyu Chu Hongxin Yang Lirong Teng Di Wang |
| author_sort | Wenqi Wang |
| collection | DOAJ |
| description | Curculigoside (CCG) is a phenolic glycoside compound extracted from the root of a natural plant called Curculigo orchioides Gaertn. In this study, the neuroprotective effect of CCG through oxidative stress mediated mitochondrial dysfunction on L-glutamate (L-Glu)-damaged hippocampal neuron cell line (HT22) and APPswe/PSEN1dE9 transgenic (APP/PS1) mice were investigated. Observably, CCG in L-Glu-damaged HT22 cells suppressed apoptosis, reduced the accumulation of reactive oxygen species, balanced the mitochondrial membrane potential and prevented the over-influx of calcium. In APP/PS1 mice, 4-week CCG administration significantly improved their memory and behavioral impairments, enhanced the function of cholinergic system, reduced the deposition of Aβ and neurofibrillary fiber tangles caused by tau phosphorylation, and suppressed the development and progression of oxidative stress in brains of APP/PS1 mice. Based on the screening of proteomic analysis on hippocampus, CCG were confirmed that it could regulate the expression levels of proteins related to mitochondrial dysfunction, mainly through activating on AMPK/Nrf2 signaling, in APP/PS1 mice and L-Glu-exposed HT22 cells. CCG has a prominent neuroprotective effect on regulate the AMPK/Nrf2-mediated mitochondrial dysfunction in cells APP/PS1 mice support CCG is a potentially potent drug for AD treatment and merits further investigation. |
| format | Article |
| id | doaj-art-b22d44749ada4bc0832d96612c35df69 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b22d44749ada4bc0832d96612c35df692025-02-03T07:06:24ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112412651278Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 miceWenqi Wang0Yidi Qu1Siyu Li2Jinyu Chu3Hongxin Yang4Lirong Teng5Di Wang6School of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Life Sciences, Jilin University, Changchun 130012, ChinaCorresponding author at: School of Life Sciences, Jilin University, Changchun 130012, China.; School of Life Sciences, Jilin University, Changchun 130012, ChinaCurculigoside (CCG) is a phenolic glycoside compound extracted from the root of a natural plant called Curculigo orchioides Gaertn. In this study, the neuroprotective effect of CCG through oxidative stress mediated mitochondrial dysfunction on L-glutamate (L-Glu)-damaged hippocampal neuron cell line (HT22) and APPswe/PSEN1dE9 transgenic (APP/PS1) mice were investigated. Observably, CCG in L-Glu-damaged HT22 cells suppressed apoptosis, reduced the accumulation of reactive oxygen species, balanced the mitochondrial membrane potential and prevented the over-influx of calcium. In APP/PS1 mice, 4-week CCG administration significantly improved their memory and behavioral impairments, enhanced the function of cholinergic system, reduced the deposition of Aβ and neurofibrillary fiber tangles caused by tau phosphorylation, and suppressed the development and progression of oxidative stress in brains of APP/PS1 mice. Based on the screening of proteomic analysis on hippocampus, CCG were confirmed that it could regulate the expression levels of proteins related to mitochondrial dysfunction, mainly through activating on AMPK/Nrf2 signaling, in APP/PS1 mice and L-Glu-exposed HT22 cells. CCG has a prominent neuroprotective effect on regulate the AMPK/Nrf2-mediated mitochondrial dysfunction in cells APP/PS1 mice support CCG is a potentially potent drug for AD treatment and merits further investigation.http://www.sciencedirect.com/science/article/pii/S2213453022002282Alzheimer's diseaseCurculigosideApoptosisOxidative stressMitochondrial dysfunctionAMPK/Nrf2 signaling |
| spellingShingle | Wenqi Wang Yidi Qu Siyu Li Jinyu Chu Hongxin Yang Lirong Teng Di Wang Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice Food Science and Human Wellness Alzheimer's disease Curculigoside Apoptosis Oxidative stress Mitochondrial dysfunction AMPK/Nrf2 signaling |
| title | Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice |
| title_full | Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice |
| title_fullStr | Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice |
| title_full_unstemmed | Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice |
| title_short | Neuroprotective effects of curculigoside against Alzheimer’s disease via regulation oxidative stress mediated mitochondrial dysfunction in L-Glu-exposed HT22 cells and APP/PS1 mice |
| title_sort | neuroprotective effects of curculigoside against alzheimer s disease via regulation oxidative stress mediated mitochondrial dysfunction in l glu exposed ht22 cells and app ps1 mice |
| topic | Alzheimer's disease Curculigoside Apoptosis Oxidative stress Mitochondrial dysfunction AMPK/Nrf2 signaling |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002282 |
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