Factors Associated With Missing Biological Samples in the Dog Aging Project
ABSTRACT Background The Dog Aging Project (DAP) is a longitudinal study examining aging in US companion dogs, where missing data pose a challenge to result validity and statistical power. Early recognition and procedural adjustments are vital to address missing samples. Objectives This research asse...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-11-01
|
| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.70113 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832593432052236288 |
|---|---|
| author | Sydney N. Holland Amanda K. Tinkle Jena N. Prescott Brianna L. Blattman Robyn L. McClelland Yunbi Nam Dog Aging Project Consortium Kate E. Creevy Virginia R. Fajt |
| author_facet | Sydney N. Holland Amanda K. Tinkle Jena N. Prescott Brianna L. Blattman Robyn L. McClelland Yunbi Nam Dog Aging Project Consortium Kate E. Creevy Virginia R. Fajt |
| author_sort | Sydney N. Holland |
| collection | DOAJ |
| description | ABSTRACT Background The Dog Aging Project (DAP) is a longitudinal study examining aging in US companion dogs, where missing data pose a challenge to result validity and statistical power. Early recognition and procedural adjustments are vital to address missing samples. Objectives This research assesses missing biospecimen samples within the PRECISION cohort of the DAP, aiming to identify contributing factors and propose mitigation strategies. Methods Ninety sample kits collected between August 2021 and October 2021 were evaluated for the missingness of individual kit components. A primary assessment reviewed the missingness of each sample type with hypothesized associated factors. A secondary assessment addressed the missingness of plasma and serum aliquots made after check‐in. Results EDTA samples for flow cytometry, peripheral blood mononuclear cells (PBMCs), DNA and biobanking were missing at rates of 18.9%, 13.3%, 6.7% and 5.6%, respectively. Faecal samples were missing at 5.6%. Urine and plasma were each missing at 2.2%. Serum, hair and dried blood spot cards were each missing at 1.1%. No statistically significant difference in missingness was seen across regions, ease of blood collection, or cooperativeness of dog during sample collection. Higher time‐to‐receipt was associated with a higher missingness proportion. Increasing missingness proportions were seen across plasma and serum aliquots that were created in sequential order. Conclusion The study suggests reevaluating sample collection instructions to address frequently missing sample types and prevent further loss. Investigation into collection procedures for these types is recommended to identify potential causes of sample yield loss. |
| format | Article |
| id | doaj-art-b22b4353ec1e464399ef14a2d185beb2 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-b22b4353ec1e464399ef14a2d185beb22025-01-20T17:16:45ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70113Factors Associated With Missing Biological Samples in the Dog Aging ProjectSydney N. Holland0Amanda K. Tinkle1Jena N. Prescott2Brianna L. Blattman3Robyn L. McClelland4Yunbi Nam5Dog Aging Project Consortium6Kate E. Creevy7Virginia R. Fajt8Department of Small Animal Clinical SciencesTexas A&M College of Veterinary Medicine & Biomedical SciencesCollege StationTexasUSADepartment of Small Animal Clinical SciencesTexas A&M College of Veterinary Medicine & Biomedical SciencesCollege StationTexasUSADepartment of Small Animal Clinical SciencesTexas A&M College of Veterinary Medicine & Biomedical SciencesCollege StationTexasUSADepartment of Small Animal Clinical SciencesTexas A&M College of Veterinary Medicine & Biomedical SciencesCollege StationTexasUSADepartment of BiostatisticsUniversity of WashingtonSeattle Washington USADepartment of BiostatisticsUniversity of WashingtonSeattle Washington USADepartment of BiostatisticsUniversity of WashingtonSeattle Washington USADepartment of Small Animal Clinical SciencesTexas A&M College of Veterinary Medicine & Biomedical SciencesCollege StationTexasUSADepartment of Veterinary Physiology and PharmacologyTexas A&M University College of Veterinary Medicine & Biomedical SciencesCollege StationTexasUSAABSTRACT Background The Dog Aging Project (DAP) is a longitudinal study examining aging in US companion dogs, where missing data pose a challenge to result validity and statistical power. Early recognition and procedural adjustments are vital to address missing samples. Objectives This research assesses missing biospecimen samples within the PRECISION cohort of the DAP, aiming to identify contributing factors and propose mitigation strategies. Methods Ninety sample kits collected between August 2021 and October 2021 were evaluated for the missingness of individual kit components. A primary assessment reviewed the missingness of each sample type with hypothesized associated factors. A secondary assessment addressed the missingness of plasma and serum aliquots made after check‐in. Results EDTA samples for flow cytometry, peripheral blood mononuclear cells (PBMCs), DNA and biobanking were missing at rates of 18.9%, 13.3%, 6.7% and 5.6%, respectively. Faecal samples were missing at 5.6%. Urine and plasma were each missing at 2.2%. Serum, hair and dried blood spot cards were each missing at 1.1%. No statistically significant difference in missingness was seen across regions, ease of blood collection, or cooperativeness of dog during sample collection. Higher time‐to‐receipt was associated with a higher missingness proportion. Increasing missingness proportions were seen across plasma and serum aliquots that were created in sequential order. Conclusion The study suggests reevaluating sample collection instructions to address frequently missing sample types and prevent further loss. Investigation into collection procedures for these types is recommended to identify potential causes of sample yield loss.https://doi.org/10.1002/vms3.70113biospecimenbloodcaninemissingness |
| spellingShingle | Sydney N. Holland Amanda K. Tinkle Jena N. Prescott Brianna L. Blattman Robyn L. McClelland Yunbi Nam Dog Aging Project Consortium Kate E. Creevy Virginia R. Fajt Factors Associated With Missing Biological Samples in the Dog Aging Project Veterinary Medicine and Science biospecimen blood canine missingness |
| title | Factors Associated With Missing Biological Samples in the Dog Aging Project |
| title_full | Factors Associated With Missing Biological Samples in the Dog Aging Project |
| title_fullStr | Factors Associated With Missing Biological Samples in the Dog Aging Project |
| title_full_unstemmed | Factors Associated With Missing Biological Samples in the Dog Aging Project |
| title_short | Factors Associated With Missing Biological Samples in the Dog Aging Project |
| title_sort | factors associated with missing biological samples in the dog aging project |
| topic | biospecimen blood canine missingness |
| url | https://doi.org/10.1002/vms3.70113 |
| work_keys_str_mv | AT sydneynholland factorsassociatedwithmissingbiologicalsamplesinthedogagingproject AT amandaktinkle factorsassociatedwithmissingbiologicalsamplesinthedogagingproject AT jenanprescott factorsassociatedwithmissingbiologicalsamplesinthedogagingproject AT briannalblattman factorsassociatedwithmissingbiologicalsamplesinthedogagingproject AT robynlmcclelland factorsassociatedwithmissingbiologicalsamplesinthedogagingproject AT yunbinam factorsassociatedwithmissingbiologicalsamplesinthedogagingproject AT dogagingprojectconsortium factorsassociatedwithmissingbiologicalsamplesinthedogagingproject AT kateecreevy factorsassociatedwithmissingbiologicalsamplesinthedogagingproject AT virginiarfajt factorsassociatedwithmissingbiologicalsamplesinthedogagingproject |