Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice
This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice. Two P. acidilactici strains with antibacterial activity (P. acidilactici CCFM28 and CCFM18) were obtaine...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2022-03-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302100104X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832557329129668608 |
|---|---|
| author | Yiteng Qiao Zhichang Qiu Fengwei Tian Leilei Yu Jianxin Zhao Hao Zhang Qixiao Zhai Wei Chen |
| author_facet | Yiteng Qiao Zhichang Qiu Fengwei Tian Leilei Yu Jianxin Zhao Hao Zhang Qixiao Zhai Wei Chen |
| author_sort | Yiteng Qiao |
| collection | DOAJ |
| description | This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice. Two P. acidilactici strains with antibacterial activity (P. acidilactici CCFM28 and CCFM18) were obtained based on the inhibition-zone assay. The produced components were identified as bacteriocins through protease treatment, pH adjustment and hydrogen peroxide treatment. Bacteriocin-producing and non-bacteriocin-producing P. acidilactici strains (P. acidilactici CCFM28, CCFM18 and NT17-3) caused significant changes in serum immune factors and intestinal flora of normal mice. After 14 days of intervention, the relative abundance of Firmicutes was significantly decreased, but that of Proteobacteria was significantly increased at the phylum level. At the genus level, the administration of three P. acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus. Furthermore, there were also different regulations on some probiotic strains, such as Bifidobacterium, Coprococcus and Akkermansia, which were closely related to the antibacterial ability of the bacteriocin and the type of strain. The results indicated that the intervention of different P. acidilactici strains could differently change the structure of intestinal flora in normal mice, which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future. |
| format | Article |
| id | doaj-art-b2187de066c142d1b70d978b47c23fcd |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-03-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-b2187de066c142d1b70d978b47c23fcd2025-02-03T05:21:16ZengTsinghua University PressFood Science and Human Wellness2213-45302022-03-01112238246Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal miceYiteng Qiao0Zhichang Qiu1Fengwei Tian2Leilei Yu3Jianxin Zhao4Hao Zhang5Qixiao Zhai6Wei Chen7State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaCollege of Food Science and Engineering, Shandong Agricultural University, Tai’an, Shandong 271018, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China; Wuxi Translational Medicine Research Center, Jiangsu Translational Medicine, Research Institute Wuxi Branch, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China; Corresponding author.This study was performed to determine the effects of bacteriocin-producing and non-bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice. Two P. acidilactici strains with antibacterial activity (P. acidilactici CCFM28 and CCFM18) were obtained based on the inhibition-zone assay. The produced components were identified as bacteriocins through protease treatment, pH adjustment and hydrogen peroxide treatment. Bacteriocin-producing and non-bacteriocin-producing P. acidilactici strains (P. acidilactici CCFM28, CCFM18 and NT17-3) caused significant changes in serum immune factors and intestinal flora of normal mice. After 14 days of intervention, the relative abundance of Firmicutes was significantly decreased, but that of Proteobacteria was significantly increased at the phylum level. At the genus level, the administration of three P. acidilactici strains resulted in the downregulation of Blautia and the upregulation of Ruminococcus and Lactobacillus. Furthermore, there were also different regulations on some probiotic strains, such as Bifidobacterium, Coprococcus and Akkermansia, which were closely related to the antibacterial ability of the bacteriocin and the type of strain. The results indicated that the intervention of different P. acidilactici strains could differently change the structure of intestinal flora in normal mice, which provided theoretical guidance for the selective use of bacteriocin-producing strains for health regulation in the future.http://www.sciencedirect.com/science/article/pii/S221345302100104XPediococcus acidilacticiBacteriocinImmune systemIntestinal floraRegulation |
| spellingShingle | Yiteng Qiao Zhichang Qiu Fengwei Tian Leilei Yu Jianxin Zhao Hao Zhang Qixiao Zhai Wei Chen Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice Food Science and Human Wellness Pediococcus acidilactici Bacteriocin Immune system Intestinal flora Regulation |
| title | Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice |
| title_full | Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice |
| title_fullStr | Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice |
| title_full_unstemmed | Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice |
| title_short | Effect of bacteriocin-producing Pediococcus acidilactici strains on the immune system and intestinal flora of normal mice |
| title_sort | effect of bacteriocin producing pediococcus acidilactici strains on the immune system and intestinal flora of normal mice |
| topic | Pediococcus acidilactici Bacteriocin Immune system Intestinal flora Regulation |
| url | http://www.sciencedirect.com/science/article/pii/S221345302100104X |
| work_keys_str_mv | AT yitengqiao effectofbacteriocinproducingpediococcusacidilacticistrainsontheimmunesystemandintestinalfloraofnormalmice AT zhichangqiu effectofbacteriocinproducingpediococcusacidilacticistrainsontheimmunesystemandintestinalfloraofnormalmice AT fengweitian effectofbacteriocinproducingpediococcusacidilacticistrainsontheimmunesystemandintestinalfloraofnormalmice AT leileiyu effectofbacteriocinproducingpediococcusacidilacticistrainsontheimmunesystemandintestinalfloraofnormalmice AT jianxinzhao effectofbacteriocinproducingpediococcusacidilacticistrainsontheimmunesystemandintestinalfloraofnormalmice AT haozhang effectofbacteriocinproducingpediococcusacidilacticistrainsontheimmunesystemandintestinalfloraofnormalmice AT qixiaozhai effectofbacteriocinproducingpediococcusacidilacticistrainsontheimmunesystemandintestinalfloraofnormalmice AT weichen effectofbacteriocinproducingpediococcusacidilacticistrainsontheimmunesystemandintestinalfloraofnormalmice |