Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs
ABSTRACT Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies h...
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Wiley
2024-11-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.70108 |
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| author | Francisco Jesus Castañeda Montes José Luis Cerriteño Sánchez Julieta Sandra Cuevas‐Romero María Azucena Castañeda Montes Susana Mendoza Elvira |
| author_facet | Francisco Jesus Castañeda Montes José Luis Cerriteño Sánchez Julieta Sandra Cuevas‐Romero María Azucena Castañeda Montes Susana Mendoza Elvira |
| author_sort | Francisco Jesus Castañeda Montes |
| collection | DOAJ |
| description | ABSTRACT Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies have shown that M protein is highly conserved and, therefore, suitable for diagnostic systems. In this study, for the first time, an indirect enzyme‐linked immunosorbent assay (iELISA) based on a recombinant M protein (rM‐PDCoV) was developed to evaluate the seroprevalence of PDCoV in four states in Mexico. High sensitivity (83%) and specificity (100%) were observed for the iELISA. The kappa index calculated a nearly perfect agreement (0.8831) compared to the Western blot (gold standard test), suggesting acceptable statistical value support. In this study, 50.38% of the serum samples from backyard pigs were PDCoV‐positive. The serological comparison showed that PDCoV/PEDV coinfections occurred in 31.98% of the analysed sera. These results can enrich our understanding of how this virus spreads and enable the evaluation of PDCoV infections. Moreover, it highlights the importance of continually investigating the seroprevalence of PDCoV in Mexico because there is also no information about the current prevalence of the disease. |
| format | Article |
| id | doaj-art-b2175b310e1e40688c70d9096bd45618 |
| institution | Kabale University |
| issn | 2053-1095 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj-art-b2175b310e1e40688c70d9096bd456182025-01-20T17:16:45ZengWileyVeterinary Medicine and Science2053-10952024-11-01106n/an/a10.1002/vms3.70108Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican PigsFrancisco Jesus Castañeda Montes0José Luis Cerriteño Sánchez1Julieta Sandra Cuevas‐Romero2María Azucena Castañeda Montes3Susana Mendoza Elvira4Estancias Posdoctorales por México para la Formación y Consolidación de las y los Investigadores por México CONAHCYT Mexico City MexicoCentro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Mexico City MexicoCentro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias Mexico City MexicoDirección de Procesos Alimentarios y Química Área Biotecnología, Unidad Académica de Capulhuac Universidad Tecnológica del Valle de Toluca Lerma MexicoPosgrado en Ciencias de la Producción y de la Salud Animal Facultad de Estudios Superiores, Universidad Nacional Autónoma de México Cuautitlan Estado de México MexicoABSTRACT Porcine deltacoronavirus (PDCoV) is an infectious disease that causes diarrhoea in pigs of different ages; however, piglets are more susceptible. PDCoV was first reported in 2012 in China and Hong Kong. Later, it was first reported in the USA in 2014 and in Mexico in 2019. Several studies have shown that M protein is highly conserved and, therefore, suitable for diagnostic systems. In this study, for the first time, an indirect enzyme‐linked immunosorbent assay (iELISA) based on a recombinant M protein (rM‐PDCoV) was developed to evaluate the seroprevalence of PDCoV in four states in Mexico. High sensitivity (83%) and specificity (100%) were observed for the iELISA. The kappa index calculated a nearly perfect agreement (0.8831) compared to the Western blot (gold standard test), suggesting acceptable statistical value support. In this study, 50.38% of the serum samples from backyard pigs were PDCoV‐positive. The serological comparison showed that PDCoV/PEDV coinfections occurred in 31.98% of the analysed sera. These results can enrich our understanding of how this virus spreads and enable the evaluation of PDCoV infections. Moreover, it highlights the importance of continually investigating the seroprevalence of PDCoV in Mexico because there is also no information about the current prevalence of the disease.https://doi.org/10.1002/vms3.70108indirect enzyme‐linked immunosorbent assay (iELISA)M proteinporcine deltacoronavirusseroprevalence |
| spellingShingle | Francisco Jesus Castañeda Montes José Luis Cerriteño Sánchez Julieta Sandra Cuevas‐Romero María Azucena Castañeda Montes Susana Mendoza Elvira Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs Veterinary Medicine and Science indirect enzyme‐linked immunosorbent assay (iELISA) M protein porcine deltacoronavirus seroprevalence |
| title | Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs |
| title_full | Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs |
| title_fullStr | Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs |
| title_full_unstemmed | Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs |
| title_short | Development and Application of an Indirect Enzyme‐Linked Immunosorbent Assay Based on a Recombinant Matrix Protein for the Serological Study of Porcine Deltacoronavirus in Mexican Pigs |
| title_sort | development and application of an indirect enzyme linked immunosorbent assay based on a recombinant matrix protein for the serological study of porcine deltacoronavirus in mexican pigs |
| topic | indirect enzyme‐linked immunosorbent assay (iELISA) M protein porcine deltacoronavirus seroprevalence |
| url | https://doi.org/10.1002/vms3.70108 |
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