Structural and Functional Characterization of Deceased Donor Stem Cells: A Viable Alternative to Living Donor Stem Cells
Stem cells can be isolated from various human tissues including bone marrow (BM) and adipose tissue (AT). Our study outlines a process to isolate adult stem cells from deceased donors. We have shown that cell counts obtained from deceased donor BM were within established living donor parameters. Eva...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2019/5841587 |
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| _version_ | 1832551483725316096 |
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| author | Prakash N. Rao Dayanand D. Deo Misty A. Marchioni Rouzbeh R. Taghizadeh Kyle Cetrulo Sharyn Sawczak Jacob Myrick |
| author_facet | Prakash N. Rao Dayanand D. Deo Misty A. Marchioni Rouzbeh R. Taghizadeh Kyle Cetrulo Sharyn Sawczak Jacob Myrick |
| author_sort | Prakash N. Rao |
| collection | DOAJ |
| description | Stem cells can be isolated from various human tissues including bone marrow (BM) and adipose tissue (AT). Our study outlines a process to isolate adult stem cells from deceased donors. We have shown that cell counts obtained from deceased donor BM were within established living donor parameters. Evaluation of demographic information exhibited a higher percentage of hematopoietic stem cells (HSC) in males versus females, as well as a higher percentage of HSC in the age bracket of 25 years and under. For the first time, we show that deceased donor femur BM grew cell colonies. Our introduction of new technology for nonenzymatic AT processing significantly increased cell recovery over the traditional enzymatic processing method. Cell counts from the deceased donor AT exceeded living donor parameters. Furthermore, our data illustrated that AT from female donors yielded a much higher number of total nucleated cells (TNC) than males. Together, our data demonstrates that our approach to isolate stem cells from deceased donors could be a routine practice to provide a viable alternative to living donor stem cells. This will offer increased accessibility for patients awaiting stem cell therapies. |
| format | Article |
| id | doaj-art-b203e41cda514419b93a0181aee53389 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-b203e41cda514419b93a0181aee533892025-02-03T06:01:26ZengWileyStem Cells International1687-966X1687-96782019-01-01201910.1155/2019/58415875841587Structural and Functional Characterization of Deceased Donor Stem Cells: A Viable Alternative to Living Donor Stem CellsPrakash N. Rao0Dayanand D. Deo1Misty A. Marchioni2Rouzbeh R. Taghizadeh3Kyle Cetrulo4Sharyn Sawczak5Jacob Myrick6NJ Sharing Network, New Providence, NJ, USANJ Sharing Network, New Providence, NJ, USANJ Sharing Network, New Providence, NJ, USAAuxoCell Laboratories, Inc., Cambridge, MA, USAAuxoCell Laboratories, Inc., Cambridge, MA, USANJ Sharing Network, New Providence, NJ, USANJ Sharing Network, New Providence, NJ, USAStem cells can be isolated from various human tissues including bone marrow (BM) and adipose tissue (AT). Our study outlines a process to isolate adult stem cells from deceased donors. We have shown that cell counts obtained from deceased donor BM were within established living donor parameters. Evaluation of demographic information exhibited a higher percentage of hematopoietic stem cells (HSC) in males versus females, as well as a higher percentage of HSC in the age bracket of 25 years and under. For the first time, we show that deceased donor femur BM grew cell colonies. Our introduction of new technology for nonenzymatic AT processing significantly increased cell recovery over the traditional enzymatic processing method. Cell counts from the deceased donor AT exceeded living donor parameters. Furthermore, our data illustrated that AT from female donors yielded a much higher number of total nucleated cells (TNC) than males. Together, our data demonstrates that our approach to isolate stem cells from deceased donors could be a routine practice to provide a viable alternative to living donor stem cells. This will offer increased accessibility for patients awaiting stem cell therapies.http://dx.doi.org/10.1155/2019/5841587 |
| spellingShingle | Prakash N. Rao Dayanand D. Deo Misty A. Marchioni Rouzbeh R. Taghizadeh Kyle Cetrulo Sharyn Sawczak Jacob Myrick Structural and Functional Characterization of Deceased Donor Stem Cells: A Viable Alternative to Living Donor Stem Cells Stem Cells International |
| title | Structural and Functional Characterization of Deceased Donor Stem Cells: A Viable Alternative to Living Donor Stem Cells |
| title_full | Structural and Functional Characterization of Deceased Donor Stem Cells: A Viable Alternative to Living Donor Stem Cells |
| title_fullStr | Structural and Functional Characterization of Deceased Donor Stem Cells: A Viable Alternative to Living Donor Stem Cells |
| title_full_unstemmed | Structural and Functional Characterization of Deceased Donor Stem Cells: A Viable Alternative to Living Donor Stem Cells |
| title_short | Structural and Functional Characterization of Deceased Donor Stem Cells: A Viable Alternative to Living Donor Stem Cells |
| title_sort | structural and functional characterization of deceased donor stem cells a viable alternative to living donor stem cells |
| url | http://dx.doi.org/10.1155/2019/5841587 |
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