<i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant Growth
Rice blast disease, caused by <i>Magnaporthe oryzae</i> (<i>M. oryzae</i>), is a significant threat to global rice production. Conventional methods for disease management face limitations, emphasizing the importance of sustainable alternatives. In this study, two rice cultiva...
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2025-01-01
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| author | Manegdebwaoga Arthur Fabrice Kabore Guanpeng Huang Changqing Feng Shuhong Wu Jiayi Guo Guofeng Wu Yiqiong Sun Samuel Tareke Woldegiorgis Yufang Ai Lina Zhang Wei Liu Huaqin He |
| author_facet | Manegdebwaoga Arthur Fabrice Kabore Guanpeng Huang Changqing Feng Shuhong Wu Jiayi Guo Guofeng Wu Yiqiong Sun Samuel Tareke Woldegiorgis Yufang Ai Lina Zhang Wei Liu Huaqin He |
| author_sort | Manegdebwaoga Arthur Fabrice Kabore |
| collection | DOAJ |
| description | Rice blast disease, caused by <i>Magnaporthe oryzae</i> (<i>M. oryzae</i>), is a significant threat to global rice production. Conventional methods for disease management face limitations, emphasizing the importance of sustainable alternatives. In this study, two rice cultivars with different blast resistant abilities, the susceptible variety CO39 and the resistant variety Pi4b, were used as materials to study the effects of <i>Piriformospora indica</i> (Pi) on the resistance to <i>M. oryzae</i> infection and rice growth. The <i>in vitro</i> tests revealed no direct antagonistic interaction between Pi and <i>M. oryzae</i>. However, the <i>in vivo</i> experiments showed that Pi promoted plant growth by increasing root and shoot length, chlorophyll content, and nitrogen uptake, particularly in CO39 during pathogen infection. Pi inoculation also significantly reduced disease severity, which was indicated by smaller lesion areas and shorter lesion lengths in both cultivars but a more pronounced effect in CO39. This occurred due to the decreasing levels of MDA and the modulating activity of antioxidant enzymes in Pi-inoculated rice plants. At the early stage of <i>M. oryzae</i> infection, the expression of the ethylene signaling gene <i>OsEIN2</i> and the gibberellin biosynthesis gene <i>OsGA20ox1</i> in Pi-inoculated CO39 decreased but significantly increased in both rice cultivars at the later stage. The reverse was found for the pathogenesis-related (PR) genes <i>OsPR10</i> and <i>OsPBZ1</i> and the blast-resistant genes <i>OsBRG1</i>, <i>OsBRG2</i>, and <i>OsBRW1</i>, suggesting early growth suppression for rice resilience to blast followed by a later shift back to growth. Meanwhile, Pi inoculation increased <i>OsCesA9</i> expression in rice to strengthen cell walls and establish the primary defense barrier against <i>M. oryzae</i> and upregulated the expression of <i>OsNPR1</i> without a significant difference in CO39 but downregulated it in Pi4b to activate PR genes to enhance plant resistance. In summary, these results underscore the potential of Pi as a sustainable biological control agent for rice blast disease, which is particularly beneficial for blast-susceptible rice cultivars. |
| format | Article |
| id | doaj-art-b1f3c94abc7242129113ee92968ee5bd |
| institution | Kabale University |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
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| spelling | doaj-art-b1f3c94abc7242129113ee92968ee5bd2025-01-24T13:16:48ZengMDPI AGAgronomy2073-43952025-01-0115111810.3390/agronomy15010118<i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant GrowthManegdebwaoga Arthur Fabrice Kabore0Guanpeng Huang1Changqing Feng2Shuhong Wu3Jiayi Guo4Guofeng Wu5Yiqiong Sun6Samuel Tareke Woldegiorgis7Yufang Ai8Lina Zhang9Wei Liu10Huaqin He11College of Juncao Sciences and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Juncao Sciences and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Juncao Sciences and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Juncao Sciences and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Juncao Sciences and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Juncao Sciences and Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaRice blast disease, caused by <i>Magnaporthe oryzae</i> (<i>M. oryzae</i>), is a significant threat to global rice production. Conventional methods for disease management face limitations, emphasizing the importance of sustainable alternatives. In this study, two rice cultivars with different blast resistant abilities, the susceptible variety CO39 and the resistant variety Pi4b, were used as materials to study the effects of <i>Piriformospora indica</i> (Pi) on the resistance to <i>M. oryzae</i> infection and rice growth. The <i>in vitro</i> tests revealed no direct antagonistic interaction between Pi and <i>M. oryzae</i>. However, the <i>in vivo</i> experiments showed that Pi promoted plant growth by increasing root and shoot length, chlorophyll content, and nitrogen uptake, particularly in CO39 during pathogen infection. Pi inoculation also significantly reduced disease severity, which was indicated by smaller lesion areas and shorter lesion lengths in both cultivars but a more pronounced effect in CO39. This occurred due to the decreasing levels of MDA and the modulating activity of antioxidant enzymes in Pi-inoculated rice plants. At the early stage of <i>M. oryzae</i> infection, the expression of the ethylene signaling gene <i>OsEIN2</i> and the gibberellin biosynthesis gene <i>OsGA20ox1</i> in Pi-inoculated CO39 decreased but significantly increased in both rice cultivars at the later stage. The reverse was found for the pathogenesis-related (PR) genes <i>OsPR10</i> and <i>OsPBZ1</i> and the blast-resistant genes <i>OsBRG1</i>, <i>OsBRG2</i>, and <i>OsBRW1</i>, suggesting early growth suppression for rice resilience to blast followed by a later shift back to growth. Meanwhile, Pi inoculation increased <i>OsCesA9</i> expression in rice to strengthen cell walls and establish the primary defense barrier against <i>M. oryzae</i> and upregulated the expression of <i>OsNPR1</i> without a significant difference in CO39 but downregulated it in Pi4b to activate PR genes to enhance plant resistance. In summary, these results underscore the potential of Pi as a sustainable biological control agent for rice blast disease, which is particularly beneficial for blast-susceptible rice cultivars.https://www.mdpi.com/2073-4395/15/1/118<i>Piriformospora indica</i><i>Magnaporthe oryzae</i>rice (<i>Oryza sativa</i> L.)balance between growth and defense |
| spellingShingle | Manegdebwaoga Arthur Fabrice Kabore Guanpeng Huang Changqing Feng Shuhong Wu Jiayi Guo Guofeng Wu Yiqiong Sun Samuel Tareke Woldegiorgis Yufang Ai Lina Zhang Wei Liu Huaqin He <i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant Growth Agronomy <i>Piriformospora indica</i> <i>Magnaporthe oryzae</i> rice (<i>Oryza sativa</i> L.) balance between growth and defense |
| title | <i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant Growth |
| title_full | <i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant Growth |
| title_fullStr | <i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant Growth |
| title_full_unstemmed | <i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant Growth |
| title_short | <i>Piriformospora indica</i> Enhances Rice Blast Resistance and Plant Growth |
| title_sort | i piriformospora indica i enhances rice blast resistance and plant growth |
| topic | <i>Piriformospora indica</i> <i>Magnaporthe oryzae</i> rice (<i>Oryza sativa</i> L.) balance between growth and defense |
| url | https://www.mdpi.com/2073-4395/15/1/118 |
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