PURITY TESTING OF ARRIAH FMD VACCINES
The paper demonstrates results of FMD vaccine testing for their inability to induce antibodies to FMD virus non-structural proteins (FMDV NSP antibodies) in cattle at different dates post vaccination. Subtype A, O, Asia-1 and SAT-2 cultural FMD virus replicated in baby hamster kidney suspension cult...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/313 |
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| author | D. A. Lozovoy D. V. Mikhalishin V. A. Starikov M. I. Doronin A. S. Sharypov |
| author_facet | D. A. Lozovoy D. V. Mikhalishin V. A. Starikov M. I. Doronin A. S. Sharypov |
| author_sort | D. A. Lozovoy |
| collection | DOAJ |
| description | The paper demonstrates results of FMD vaccine testing for their inability to induce antibodies to FMD virus non-structural proteins (FMDV NSP antibodies) in cattle at different dates post vaccination. Subtype A, O, Asia-1 and SAT-2 cultural FMD virus replicated in baby hamster kidney suspension culture (ВНК-21/2-17) was used for vaccine production. FMDV antigen was inactivated with aminoethyl ethylenimine solution, and the suspension was subsequently purified. Non NSP-purified preparation was made to simulate presence of NSPs in the vaccine. ELISA test-kits were used for testing cattle sera collected before and after immunization for presence of 3ABC polyprotein-specific antibodies. Inoculation of cattle with non-purified preparation induced antibodies specific to FMDV NSPs in 87.5% animals on day 30, in 75% animals on day 44 and in 62.5% animals on day 132 post third immunization. All tested vaccines manufactured by the FGBI “ARRIAH” were demonstrated to induce no FMDV NSP antibodies suggesting their complete removal from the virus-containing suspension. |
| format | Article |
| id | doaj-art-b1ee6a5b4ca8444b9ec0fa072f852ebd |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-b1ee6a5b4ca8444b9ec0fa072f852ebd2025-02-06T09:52:06ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-0103612313PURITY TESTING OF ARRIAH FMD VACCINESD. A. Lozovoy0D. V. Mikhalishin1V. A. Starikov2M. I. Doronin3A. S. Sharypov4FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThe paper demonstrates results of FMD vaccine testing for their inability to induce antibodies to FMD virus non-structural proteins (FMDV NSP antibodies) in cattle at different dates post vaccination. Subtype A, O, Asia-1 and SAT-2 cultural FMD virus replicated in baby hamster kidney suspension culture (ВНК-21/2-17) was used for vaccine production. FMDV antigen was inactivated with aminoethyl ethylenimine solution, and the suspension was subsequently purified. Non NSP-purified preparation was made to simulate presence of NSPs in the vaccine. ELISA test-kits were used for testing cattle sera collected before and after immunization for presence of 3ABC polyprotein-specific antibodies. Inoculation of cattle with non-purified preparation induced antibodies specific to FMDV NSPs in 87.5% animals on day 30, in 75% animals on day 44 and in 62.5% animals on day 132 post third immunization. All tested vaccines manufactured by the FGBI “ARRIAH” were demonstrated to induce no FMDV NSP antibodies suggesting their complete removal from the virus-containing suspension.https://veterinary.arriah.ru/jour/article/view/313антителанеструктурные белки вируса ящурапротивоящурная вакцинаклеточный детритиммуноферментный анализantibodiesfmdv non-structural proteinsfmd vaccinecell debriselisa |
| spellingShingle | D. A. Lozovoy D. V. Mikhalishin V. A. Starikov M. I. Doronin A. S. Sharypov PURITY TESTING OF ARRIAH FMD VACCINES Ветеринария сегодня антитела неструктурные белки вируса ящура противоящурная вакцина клеточный детрит иммуноферментный анализ antibodies fmdv non-structural proteins fmd vaccine cell debris elisa |
| title | PURITY TESTING OF ARRIAH FMD VACCINES |
| title_full | PURITY TESTING OF ARRIAH FMD VACCINES |
| title_fullStr | PURITY TESTING OF ARRIAH FMD VACCINES |
| title_full_unstemmed | PURITY TESTING OF ARRIAH FMD VACCINES |
| title_short | PURITY TESTING OF ARRIAH FMD VACCINES |
| title_sort | purity testing of arriah fmd vaccines |
| topic | антитела неструктурные белки вируса ящура противоящурная вакцина клеточный детрит иммуноферментный анализ antibodies fmdv non-structural proteins fmd vaccine cell debris elisa |
| url | https://veterinary.arriah.ru/jour/article/view/313 |
| work_keys_str_mv | AT dalozovoy puritytestingofarriahfmdvaccines AT dvmikhalishin puritytestingofarriahfmdvaccines AT vastarikov puritytestingofarriahfmdvaccines AT midoronin puritytestingofarriahfmdvaccines AT assharypov puritytestingofarriahfmdvaccines |