A quick-to-implement and optimized CRISPR-Cas9 protocol to obtain insertional and small indel mutants in Chlamydomonas reinhardtii
Chlamydomonas reinhardtii is a leading model organism in algal research, widely used to study photosynthesis, chloroplast and cilia biology, and more recently, metabolism, light signaling, the cell cycle, and algal biotechnology. Its sequenced genome has significantly accelerated research in the fie...
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| Main Authors: | Mariano A. De Silvio, Camila Sánchez-Retuerta, M. Águila Ruiz-Sola, Olga Baidukova, Elena Monte |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-12-01
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| Series: | MethodsX |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016125002626 |
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