Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells
Background. The current treatment for postoperative hypoparathyroidism has shortcomings, such as repeated blood monitoring for dosage adjustment, uncertain long-term efficacy, and the high price of recombinant parathyroid hormone therapy. Adipose-derived stem cells can undergo adipogenic and osteoge...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2020/1860842 |
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| author | Ping Zhang Hao Zhang Wenwu Dong Zhihong Wang Yuan Qin Changhao Wu Qi Dong |
| author_facet | Ping Zhang Hao Zhang Wenwu Dong Zhihong Wang Yuan Qin Changhao Wu Qi Dong |
| author_sort | Ping Zhang |
| collection | DOAJ |
| description | Background. The current treatment for postoperative hypoparathyroidism has shortcomings, such as repeated blood monitoring for dosage adjustment, uncertain long-term efficacy, and the high price of recombinant parathyroid hormone therapy. Adipose-derived stem cells can undergo adipogenic and osteogenic differentiation in vitro and are considered a novel source of parathyroid-like cells, but the idea lacks theoretical basis and feasibility. We aimed at establishing a protocol for differentiating adipose-derived stem cells into parathyroid-like cells for treating hypoparathyroidism. Materials/Methods. Adipose-derived stem cells were isolated and purified from the inguinal adipose tissue of Sprague Dawley rats. Adipogenic differentiation and osteogenic differentiation of the cells were identified by oil red O and alizarin red S staining, respectively. The adipose-derived stem cells were stimulated by sonic hedgehog (SHH) and activin A. The differentiation of the adipose-derived stem cells to parathyroid-like cells was confirmed by the detection of parathyroid hormone and the related parathyroid markers. Results. Adipose-derived stem cells were successfully isolated and purified from the rat adipocytes. The adipogenic and osteogenic differentiation capabilities of the adipose-derived stem cells were determined. SHH and activin A stimulated parathyroid hormone secretion by the adipose-derived stem cells and significantly increased the expression of calcium-sensing receptor (CaSR), parathyroid hormone, and glial cells missing homolog 2 (GCM2) in the cells in a time- and concentration-dependent manner. Conclusion. We successfully differentiated rat adipose-derived stem cells into parathyroid-like cells, which will pave a new route to curing hypoparathyroidism. |
| format | Article |
| id | doaj-art-b1dd6f73942347f1b4fcc731ce6a5a69 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-b1dd6f73942347f1b4fcc731ce6a5a692025-02-03T06:46:41ZengWileyInternational Journal of Endocrinology1687-83371687-83452020-01-01202010.1155/2020/18608421860842Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like CellsPing Zhang0Hao Zhang1Wenwu Dong2Zhihong Wang3Yuan Qin4Changhao Wu5Qi Dong6Department of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, ChinaDepartment of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, ChinaDepartment of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, ChinaDepartment of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, ChinaDepartment of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, ChinaDepartment of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, ChinaDepartment of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, ChinaBackground. The current treatment for postoperative hypoparathyroidism has shortcomings, such as repeated blood monitoring for dosage adjustment, uncertain long-term efficacy, and the high price of recombinant parathyroid hormone therapy. Adipose-derived stem cells can undergo adipogenic and osteogenic differentiation in vitro and are considered a novel source of parathyroid-like cells, but the idea lacks theoretical basis and feasibility. We aimed at establishing a protocol for differentiating adipose-derived stem cells into parathyroid-like cells for treating hypoparathyroidism. Materials/Methods. Adipose-derived stem cells were isolated and purified from the inguinal adipose tissue of Sprague Dawley rats. Adipogenic differentiation and osteogenic differentiation of the cells were identified by oil red O and alizarin red S staining, respectively. The adipose-derived stem cells were stimulated by sonic hedgehog (SHH) and activin A. The differentiation of the adipose-derived stem cells to parathyroid-like cells was confirmed by the detection of parathyroid hormone and the related parathyroid markers. Results. Adipose-derived stem cells were successfully isolated and purified from the rat adipocytes. The adipogenic and osteogenic differentiation capabilities of the adipose-derived stem cells were determined. SHH and activin A stimulated parathyroid hormone secretion by the adipose-derived stem cells and significantly increased the expression of calcium-sensing receptor (CaSR), parathyroid hormone, and glial cells missing homolog 2 (GCM2) in the cells in a time- and concentration-dependent manner. Conclusion. We successfully differentiated rat adipose-derived stem cells into parathyroid-like cells, which will pave a new route to curing hypoparathyroidism.http://dx.doi.org/10.1155/2020/1860842 |
| spellingShingle | Ping Zhang Hao Zhang Wenwu Dong Zhihong Wang Yuan Qin Changhao Wu Qi Dong Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells International Journal of Endocrinology |
| title | Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells |
| title_full | Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells |
| title_fullStr | Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells |
| title_full_unstemmed | Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells |
| title_short | Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells |
| title_sort | differentiation of rat adipose derived stem cells into parathyroid like cells |
| url | http://dx.doi.org/10.1155/2020/1860842 |
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