Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary
The progressive decline of the ovarian follicle pool leads to reproductive ageing. The latter is accompanied by age-related disorders, including various types of cancer. In fact, the highest rates of ovarian cancer (OC) occur at postmenopause while OC risk is significantly modulated by parity record...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2018/1289103 |
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| _version_ | 1832558952850653184 |
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| author | Ulises Urzua Carlos Chacon Renato Espinoza Sebastián Martínez Nicole Hernandez |
| author_facet | Ulises Urzua Carlos Chacon Renato Espinoza Sebastián Martínez Nicole Hernandez |
| author_sort | Ulises Urzua |
| collection | DOAJ |
| description | The progressive decline of the ovarian follicle pool leads to reproductive ageing. The latter is accompanied by age-related disorders, including various types of cancer. In fact, the highest rates of ovarian cancer (OC) occur at postmenopause while OC risk is significantly modulated by parity records during previous fertile life. We approached the age-parity relationship in the C57BL/6 mouse model and herein describe the presence of nonheme iron (hemosiderin) and deposits of the “age pigment” lipofuscin in reproductively aged mouse ovaries by applying conventional histochemical methods and autofluorescence. In addition, the 8-OHdG adduct was evaluated in ovarian genomic DNA. Both hemosiderin and lipofuscin were significantly higher in virgin compared to multiparous ovaries. The same pattern was observed for 8-OHdG. We conclude that nulliparity induces a long-term accumulation of iron and lipofuscin with concomitant oxidative damage to DNA in the mouse ovary. Since lipofuscin is a widely accepted senescence marker and given the recently postulated role of lipofuscin-associated iron as a source of reactive oxygen species (ROS) in senescent cells, these findings suggest a possible pathogenic mechanism by which nulliparity contributes to an increased OC risk in the postmenopausal ovary. |
| format | Article |
| id | doaj-art-b1c06aec5d9e441298a17df705f157a7 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-b1c06aec5d9e441298a17df705f157a72025-02-03T01:31:17ZengWileyAnalytical Cellular Pathology2210-71772210-71852018-01-01201810.1155/2018/12891031289103Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse OvaryUlises Urzua0Carlos Chacon1Renato Espinoza2Sebastián Martínez3Nicole Hernandez4Laboratorio de Genómica Aplicada, Departamento de Oncología Básica y Clínica, Facultad de Medicina, Universidad de Chile, Santiago, ChileLaboratorio de Genómica Aplicada, Departamento de Oncología Básica y Clínica, Facultad de Medicina, Universidad de Chile, Santiago, ChileLaboratorio de Genómica Aplicada, Departamento de Oncología Básica y Clínica, Facultad de Medicina, Universidad de Chile, Santiago, ChileLaboratorio de Genómica Aplicada, Departamento de Oncología Básica y Clínica, Facultad de Medicina, Universidad de Chile, Santiago, ChileLaboratorio de Genómica Aplicada, Departamento de Oncología Básica y Clínica, Facultad de Medicina, Universidad de Chile, Santiago, ChileThe progressive decline of the ovarian follicle pool leads to reproductive ageing. The latter is accompanied by age-related disorders, including various types of cancer. In fact, the highest rates of ovarian cancer (OC) occur at postmenopause while OC risk is significantly modulated by parity records during previous fertile life. We approached the age-parity relationship in the C57BL/6 mouse model and herein describe the presence of nonheme iron (hemosiderin) and deposits of the “age pigment” lipofuscin in reproductively aged mouse ovaries by applying conventional histochemical methods and autofluorescence. In addition, the 8-OHdG adduct was evaluated in ovarian genomic DNA. Both hemosiderin and lipofuscin were significantly higher in virgin compared to multiparous ovaries. The same pattern was observed for 8-OHdG. We conclude that nulliparity induces a long-term accumulation of iron and lipofuscin with concomitant oxidative damage to DNA in the mouse ovary. Since lipofuscin is a widely accepted senescence marker and given the recently postulated role of lipofuscin-associated iron as a source of reactive oxygen species (ROS) in senescent cells, these findings suggest a possible pathogenic mechanism by which nulliparity contributes to an increased OC risk in the postmenopausal ovary.http://dx.doi.org/10.1155/2018/1289103 |
| spellingShingle | Ulises Urzua Carlos Chacon Renato Espinoza Sebastián Martínez Nicole Hernandez Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary Analytical Cellular Pathology |
| title | Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary |
| title_full | Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary |
| title_fullStr | Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary |
| title_full_unstemmed | Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary |
| title_short | Parity-Dependent Hemosiderin and Lipofuscin Accumulation in the Reproductively Aged Mouse Ovary |
| title_sort | parity dependent hemosiderin and lipofuscin accumulation in the reproductively aged mouse ovary |
| url | http://dx.doi.org/10.1155/2018/1289103 |
| work_keys_str_mv | AT ulisesurzua paritydependenthemosiderinandlipofuscinaccumulationinthereproductivelyagedmouseovary AT carloschacon paritydependenthemosiderinandlipofuscinaccumulationinthereproductivelyagedmouseovary AT renatoespinoza paritydependenthemosiderinandlipofuscinaccumulationinthereproductivelyagedmouseovary AT sebastianmartinez paritydependenthemosiderinandlipofuscinaccumulationinthereproductivelyagedmouseovary AT nicolehernandez paritydependenthemosiderinandlipofuscinaccumulationinthereproductivelyagedmouseovary |