Highly effective treatment of bacterial infection-accompanied wounds by fat extract-embedded phototherapeutic hydrogel
Abstract Phototherapy presents an effective approach for treating localized methicillin-resistant Staphylococcus aureus (MRSA) infections; however, the tradeoff between therapeutic efficacy and negative off-target effect persists. To address these issues, we have developed a nanoparticle-hydrogel su...
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| Format: | Article |
| Language: | English |
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BMC
2025-01-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03093-8 |
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| author | Haonan Wang Yingnan Xiao Deli Zhuge Genghe Shi Chengping Liu Hui Liang Jing Wu Yunxuan Huang Yilin Xie Chunnan Hu Jiafeng Xie Xinyi Wang Yao Yao Fang Wang Xufei Zhang Chengke Huang Yingzheng Zhao Weiyang Meng Yijie Chen Mengchun Chen |
| author_facet | Haonan Wang Yingnan Xiao Deli Zhuge Genghe Shi Chengping Liu Hui Liang Jing Wu Yunxuan Huang Yilin Xie Chunnan Hu Jiafeng Xie Xinyi Wang Yao Yao Fang Wang Xufei Zhang Chengke Huang Yingzheng Zhao Weiyang Meng Yijie Chen Mengchun Chen |
| author_sort | Haonan Wang |
| collection | DOAJ |
| description | Abstract Phototherapy presents an effective approach for treating localized methicillin-resistant Staphylococcus aureus (MRSA) infections; however, the tradeoff between therapeutic efficacy and negative off-target effect persists. To address these issues, we have developed a nanoparticle-hydrogel superstructure comprising phototherapeutic liposomal nanobubbles (NB) and fat extract (FE) encapsulated by F-127 hydrogel. After local administration to sites of MRSA infection, the superstructure effectively neutralizes high levels of MRSA toxins to protect against toxin-mediated cytotoxicity through loaded, which can also be leveraged to enhance anti-MRSA efficacy via toxin-regulated on-demand phototherapy upon near-infrared irradiation. Meanwhile, the oxidative stress-induced injury to healthy cells can be mitigated by the FE. In a murine model of skin MRSA infection, treatment with the nanoparticle-hydrogel superstructure significantly reduces MRSA load, especially when combined with MRSA toxin for enhanced bacterial inhibition. Concurrently, this superstructure accelerates wound healing by enhancing angiogenesis and collagen deposition while reducing inflammation. Overall, the nanoparticle-hydrogel superstructure shows promise for treating local pathogen-infected wounds. |
| format | Article |
| id | doaj-art-b1aab5dd05e746d5857364ed3ff33a32 |
| institution | Kabale University |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-b1aab5dd05e746d5857364ed3ff33a322025-01-26T12:50:51ZengBMCJournal of Nanobiotechnology1477-31552025-01-0123111610.1186/s12951-025-03093-8Highly effective treatment of bacterial infection-accompanied wounds by fat extract-embedded phototherapeutic hydrogelHaonan Wang0Yingnan Xiao1Deli Zhuge2Genghe Shi3Chengping Liu4Hui Liang5Jing Wu6Yunxuan Huang7Yilin Xie8Chunnan Hu9Jiafeng Xie10Xinyi Wang11Yao Yao12Fang Wang13Xufei Zhang14Chengke Huang15Yingzheng Zhao16Weiyang Meng17Yijie Chen18Mengchun Chen19Department of Pharmacy, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Wenzhou Medical UniversityDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Wenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityWenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Wenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Wenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityWenzhou Medical UniversityDepartment of Pharmacy, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Pharmaceutics, School of Pharmaceutical Sciences, Wenzhou Medical UniversityDepartment of Emergency Medicine, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Obstetrics and Gynecology, The Second Affiliated Hospital of Wenzhou Medical UniversityDepartment of Pharmacy, The Second Affiliated Hospital of Wenzhou Medical UniversityAbstract Phototherapy presents an effective approach for treating localized methicillin-resistant Staphylococcus aureus (MRSA) infections; however, the tradeoff between therapeutic efficacy and negative off-target effect persists. To address these issues, we have developed a nanoparticle-hydrogel superstructure comprising phototherapeutic liposomal nanobubbles (NB) and fat extract (FE) encapsulated by F-127 hydrogel. After local administration to sites of MRSA infection, the superstructure effectively neutralizes high levels of MRSA toxins to protect against toxin-mediated cytotoxicity through loaded, which can also be leveraged to enhance anti-MRSA efficacy via toxin-regulated on-demand phototherapy upon near-infrared irradiation. Meanwhile, the oxidative stress-induced injury to healthy cells can be mitigated by the FE. In a murine model of skin MRSA infection, treatment with the nanoparticle-hydrogel superstructure significantly reduces MRSA load, especially when combined with MRSA toxin for enhanced bacterial inhibition. Concurrently, this superstructure accelerates wound healing by enhancing angiogenesis and collagen deposition while reducing inflammation. Overall, the nanoparticle-hydrogel superstructure shows promise for treating local pathogen-infected wounds.https://doi.org/10.1186/s12951-025-03093-8Methicillin-resistant Staphylococcus aureusBacterial infection-accompanied woundsPhototherapyHydrogelWound healing |
| spellingShingle | Haonan Wang Yingnan Xiao Deli Zhuge Genghe Shi Chengping Liu Hui Liang Jing Wu Yunxuan Huang Yilin Xie Chunnan Hu Jiafeng Xie Xinyi Wang Yao Yao Fang Wang Xufei Zhang Chengke Huang Yingzheng Zhao Weiyang Meng Yijie Chen Mengchun Chen Highly effective treatment of bacterial infection-accompanied wounds by fat extract-embedded phototherapeutic hydrogel Journal of Nanobiotechnology Methicillin-resistant Staphylococcus aureus Bacterial infection-accompanied wounds Phototherapy Hydrogel Wound healing |
| title | Highly effective treatment of bacterial infection-accompanied wounds by fat extract-embedded phototherapeutic hydrogel |
| title_full | Highly effective treatment of bacterial infection-accompanied wounds by fat extract-embedded phototherapeutic hydrogel |
| title_fullStr | Highly effective treatment of bacterial infection-accompanied wounds by fat extract-embedded phototherapeutic hydrogel |
| title_full_unstemmed | Highly effective treatment of bacterial infection-accompanied wounds by fat extract-embedded phototherapeutic hydrogel |
| title_short | Highly effective treatment of bacterial infection-accompanied wounds by fat extract-embedded phototherapeutic hydrogel |
| title_sort | highly effective treatment of bacterial infection accompanied wounds by fat extract embedded phototherapeutic hydrogel |
| topic | Methicillin-resistant Staphylococcus aureus Bacterial infection-accompanied wounds Phototherapy Hydrogel Wound healing |
| url | https://doi.org/10.1186/s12951-025-03093-8 |
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