MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus
The role of microRNA (miRNA) in gestational diabetes mellitus has been widely investigated during the last decade. However, the altering effect of miR-6869-5p on immunity and placental microenvironment in gestational diabetes mellitus is largely unknown. In our study, the expression of miR-6869-5p w...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/6696636 |
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| author | Pingping Wang Zhenzhi Ma Zengyan Wang Ximei Wang Guifeng Zhao Zengfang Wang |
| author_facet | Pingping Wang Zhenzhi Ma Zengyan Wang Ximei Wang Guifeng Zhao Zengfang Wang |
| author_sort | Pingping Wang |
| collection | DOAJ |
| description | The role of microRNA (miRNA) in gestational diabetes mellitus has been widely investigated during the last decade. However, the altering effect of miR-6869-5p on immunity and placental microenvironment in gestational diabetes mellitus is largely unknown. In our study, the expression of miR-6869-5p was documented to be significantly decreased in placenta-derived mononuclear macrophages, which was also negatively related to PTPRO. Besides, PTPRO was negatively regulated by miR-6869-5p in placenta-derived mononuclear macrophages. In vitro, miR-6869-5p inhibited macrophage proliferation demonstrated by EdU and CCK-8 experiments. The inflammatory response in macrophages was also significantly inhibited by miR-6869-5p, which could regulate PTPRO as a target documented by luciferase reporter assay. Moreover, miR-6869-5p promoted M2 macrophage polarization and thus restrain inflammation. Accordingly, miR-6869-5p is involved in maintaining placental microenvironment balance by preventing from inflammation and inducing M2 macrophages in gestational diabetes mellitus. |
| format | Article |
| id | doaj-art-b1a9d8a10038439f8d06d2ad519dd2c4 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b1a9d8a10038439f8d06d2ad519dd2c42025-02-03T05:49:18ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/66966366696636MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes MellitusPingping Wang0Zhenzhi Ma1Zengyan Wang2Ximei Wang3Guifeng Zhao4Zengfang Wang5Department of Gynecology and Obstetrics, the Affiliated Hospital of Maternal and Child Health, Weifang Medical University, Weifang 261000, ChinaDepartment of Pharmacy, the First Affiliated Hospital of Weifang Medical University, Weifang 261000, ChinaOperating Room, Zhucheng People’s Hospital, Zhucheng 262200, ChinaDepartment of Gynecology and Obstetrics, the Affiliated Hospital of Maternal and Child Health, Weifang Medical University, Weifang 261000, ChinaDepartment of Antenatal Diagnosis, the First Affiliated Hospital of Maternal and Child Health, Weifang Medical University, Weifang 261000, ChinaDepartment of Gynecology and Obstetrics, the Affiliated Hospital of Maternal and Child Health, Weifang Medical University, Weifang 261000, ChinaThe role of microRNA (miRNA) in gestational diabetes mellitus has been widely investigated during the last decade. However, the altering effect of miR-6869-5p on immunity and placental microenvironment in gestational diabetes mellitus is largely unknown. In our study, the expression of miR-6869-5p was documented to be significantly decreased in placenta-derived mononuclear macrophages, which was also negatively related to PTPRO. Besides, PTPRO was negatively regulated by miR-6869-5p in placenta-derived mononuclear macrophages. In vitro, miR-6869-5p inhibited macrophage proliferation demonstrated by EdU and CCK-8 experiments. The inflammatory response in macrophages was also significantly inhibited by miR-6869-5p, which could regulate PTPRO as a target documented by luciferase reporter assay. Moreover, miR-6869-5p promoted M2 macrophage polarization and thus restrain inflammation. Accordingly, miR-6869-5p is involved in maintaining placental microenvironment balance by preventing from inflammation and inducing M2 macrophages in gestational diabetes mellitus.http://dx.doi.org/10.1155/2021/6696636 |
| spellingShingle | Pingping Wang Zhenzhi Ma Zengyan Wang Ximei Wang Guifeng Zhao Zengfang Wang MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus Mediators of Inflammation |
| title | MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus |
| title_full | MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus |
| title_fullStr | MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus |
| title_full_unstemmed | MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus |
| title_short | MiR-6869-5p Induces M2 Polarization by Regulating PTPRO in Gestational Diabetes Mellitus |
| title_sort | mir 6869 5p induces m2 polarization by regulating ptpro in gestational diabetes mellitus |
| url | http://dx.doi.org/10.1155/2021/6696636 |
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