Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
Glycosylation of immunoglobulin G (IgG) influences IgG effector function by modulating binding to Fc receptors. To identify genetic loci associated with IgG glycosylation, we quantitated N-linked IgG glycans using two approaches. After isolating IgG from human plasma, we performed 77 quantitative me...
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS Genetics |
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| author | Gordan Lauc Jennifer E Huffman Maja Pučić Lina Zgaga Barbara Adamczyk Ana Mužinić Mislav Novokmet Ozren Polašek Olga Gornik Jasminka Krištić Toma Keser Veronique Vitart Blanca Scheijen Hae-Won Uh Mariam Molokhia Alan Leslie Patrick Paul McKeigue Ivana Kolčić Ivan Krešimir Lukić Olivia Swann Frank N van Leeuwen L Renee Ruhaak Jeanine J Houwing-Duistermaat P Eline Slagboom Marian Beekman Anton J M de Craen André M Deelder Qiang Zeng Wei Wang Nicholas D Hastie Ulf Gyllensten James F Wilson Manfred Wuhrer Alan F Wright Pauline M Rudd Caroline Hayward Yurii Aulchenko Harry Campbell Igor Rudan |
| author_facet | Gordan Lauc Jennifer E Huffman Maja Pučić Lina Zgaga Barbara Adamczyk Ana Mužinić Mislav Novokmet Ozren Polašek Olga Gornik Jasminka Krištić Toma Keser Veronique Vitart Blanca Scheijen Hae-Won Uh Mariam Molokhia Alan Leslie Patrick Paul McKeigue Ivana Kolčić Ivan Krešimir Lukić Olivia Swann Frank N van Leeuwen L Renee Ruhaak Jeanine J Houwing-Duistermaat P Eline Slagboom Marian Beekman Anton J M de Craen André M Deelder Qiang Zeng Wei Wang Nicholas D Hastie Ulf Gyllensten James F Wilson Manfred Wuhrer Alan F Wright Pauline M Rudd Caroline Hayward Yurii Aulchenko Harry Campbell Igor Rudan |
| author_sort | Gordan Lauc |
| collection | DOAJ |
| description | Glycosylation of immunoglobulin G (IgG) influences IgG effector function by modulating binding to Fc receptors. To identify genetic loci associated with IgG glycosylation, we quantitated N-linked IgG glycans using two approaches. After isolating IgG from human plasma, we performed 77 quantitative measurements of N-glycosylation using ultra-performance liquid chromatography (UPLC) in 2,247 individuals from four European discovery populations. In parallel, we measured IgG N-glycans using MALDI-TOF mass spectrometry (MS) in a replication cohort of 1,848 Europeans. Meta-analysis of genome-wide association study (GWAS) results identified 9 genome-wide significant loci (P<2.27 × 10(-9)) in the discovery analysis and two of the same loci (B4GALT1 and MGAT3) in the replication cohort. Four loci contained genes encoding glycosyltransferases (ST6GAL1, B4GALT1, FUT8, and MGAT3), while the remaining 5 contained genes that have not been previously implicated in protein glycosylation (IKZF1, IL6ST-ANKRD55, ABCF2-SMARCD3, SUV420H1, and SMARCB1-DERL3). However, most of them have been strongly associated with autoimmune and inflammatory conditions (e.g., systemic lupus erythematosus, rheumatoid arthritis, ulcerative colitis, Crohn's disease, diabetes type 1, multiple sclerosis, Graves' disease, celiac disease, nodular sclerosis) and/or haematological cancers (acute lymphoblastic leukaemia, Hodgkin lymphoma, and multiple myeloma). Follow-up functional experiments in haplodeficient Ikzf1 knock-out mice showed the same general pattern of changes in IgG glycosylation as identified in the meta-analysis. As IKZF1 was associated with multiple IgG N-glycan traits, we explored biomarker potential of affected N-glycans in 101 cases with SLE and 183 matched controls and demonstrated substantial discriminative power in a ROC-curve analysis (area under the curve = 0.842). Our study shows that it is possible to identify new loci that control glycosylation of a single plasma protein using GWAS. The results may also provide an explanation for the reported pleiotropy and antagonistic effects of loci involved in autoimmune diseases and haematological cancer. |
| format | Article |
| id | doaj-art-b199e4ae942e4dc2a7fbc3d1609faf09 |
| institution | OA Journals |
| issn | 1553-7390 1553-7404 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj-art-b199e4ae942e4dc2a7fbc3d1609faf092025-08-20T02:05:37ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-01-0191e100322510.1371/journal.pgen.1003225Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.Gordan LaucJennifer E HuffmanMaja PučićLina ZgagaBarbara AdamczykAna MužinićMislav NovokmetOzren PolašekOlga GornikJasminka KrištićToma KeserVeronique VitartBlanca ScheijenHae-Won UhMariam MolokhiaAlan Leslie PatrickPaul McKeigueIvana KolčićIvan Krešimir LukićOlivia SwannFrank N van LeeuwenL Renee RuhaakJeanine J Houwing-DuistermaatP Eline SlagboomMarian BeekmanAnton J M de CraenAndré M DeelderQiang ZengWei WangNicholas D HastieUlf GyllenstenJames F WilsonManfred WuhrerAlan F WrightPauline M RuddCaroline HaywardYurii AulchenkoHarry CampbellIgor RudanGlycosylation of immunoglobulin G (IgG) influences IgG effector function by modulating binding to Fc receptors. To identify genetic loci associated with IgG glycosylation, we quantitated N-linked IgG glycans using two approaches. After isolating IgG from human plasma, we performed 77 quantitative measurements of N-glycosylation using ultra-performance liquid chromatography (UPLC) in 2,247 individuals from four European discovery populations. In parallel, we measured IgG N-glycans using MALDI-TOF mass spectrometry (MS) in a replication cohort of 1,848 Europeans. Meta-analysis of genome-wide association study (GWAS) results identified 9 genome-wide significant loci (P<2.27 × 10(-9)) in the discovery analysis and two of the same loci (B4GALT1 and MGAT3) in the replication cohort. Four loci contained genes encoding glycosyltransferases (ST6GAL1, B4GALT1, FUT8, and MGAT3), while the remaining 5 contained genes that have not been previously implicated in protein glycosylation (IKZF1, IL6ST-ANKRD55, ABCF2-SMARCD3, SUV420H1, and SMARCB1-DERL3). However, most of them have been strongly associated with autoimmune and inflammatory conditions (e.g., systemic lupus erythematosus, rheumatoid arthritis, ulcerative colitis, Crohn's disease, diabetes type 1, multiple sclerosis, Graves' disease, celiac disease, nodular sclerosis) and/or haematological cancers (acute lymphoblastic leukaemia, Hodgkin lymphoma, and multiple myeloma). Follow-up functional experiments in haplodeficient Ikzf1 knock-out mice showed the same general pattern of changes in IgG glycosylation as identified in the meta-analysis. As IKZF1 was associated with multiple IgG N-glycan traits, we explored biomarker potential of affected N-glycans in 101 cases with SLE and 183 matched controls and demonstrated substantial discriminative power in a ROC-curve analysis (area under the curve = 0.842). Our study shows that it is possible to identify new loci that control glycosylation of a single plasma protein using GWAS. The results may also provide an explanation for the reported pleiotropy and antagonistic effects of loci involved in autoimmune diseases and haematological cancer.https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003225&type=printable |
| spellingShingle | Gordan Lauc Jennifer E Huffman Maja Pučić Lina Zgaga Barbara Adamczyk Ana Mužinić Mislav Novokmet Ozren Polašek Olga Gornik Jasminka Krištić Toma Keser Veronique Vitart Blanca Scheijen Hae-Won Uh Mariam Molokhia Alan Leslie Patrick Paul McKeigue Ivana Kolčić Ivan Krešimir Lukić Olivia Swann Frank N van Leeuwen L Renee Ruhaak Jeanine J Houwing-Duistermaat P Eline Slagboom Marian Beekman Anton J M de Craen André M Deelder Qiang Zeng Wei Wang Nicholas D Hastie Ulf Gyllensten James F Wilson Manfred Wuhrer Alan F Wright Pauline M Rudd Caroline Hayward Yurii Aulchenko Harry Campbell Igor Rudan Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers. PLoS Genetics |
| title | Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers. |
| title_full | Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers. |
| title_fullStr | Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers. |
| title_full_unstemmed | Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers. |
| title_short | Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers. |
| title_sort | loci associated with n glycosylation of human immunoglobulin g show pleiotropy with autoimmune diseases and haematological cancers |
| url | https://journals.plos.org/plosgenetics/article/file?id=10.1371/journal.pgen.1003225&type=printable |
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