The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model
The effects of cyclooxygenase (COX) inhibition following the reconstruction of the anterior cruciate ligament remain unclear. We examined the effects of selective COX-2 and nonselective COX inhibition on bone-tendon integration in an in vitro model. We measured the dose-dependent effects of ibuprofe...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/926369 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558549679472640 |
|---|---|
| author | Tim Schwarting Sebastian Pretzsch Florian Debus Steffen Ruchholtz Philipp Lechler |
| author_facet | Tim Schwarting Sebastian Pretzsch Florian Debus Steffen Ruchholtz Philipp Lechler |
| author_sort | Tim Schwarting |
| collection | DOAJ |
| description | The effects of cyclooxygenase (COX) inhibition following the reconstruction of the anterior cruciate ligament remain unclear. We examined the effects of selective COX-2 and nonselective COX inhibition on bone-tendon integration in an in vitro model. We measured the dose-dependent effects of ibuprofen and parecoxib on the viability of lipopolysaccharide- (LPS-) stimulated and unstimulated mouse MC3T3-E1 and 3T3 cells, the influence on gene expression at the osteoblast, interface, and fibroblast regions measured by quantitative PCR, and cellular outgrowth assessed on histological sections. Ibuprofen led to a dose-dependent suppression of MC3T3 cell viability, while parecoxib reduced the viability of 3T3 cultures. Exposure to ibuprofen significantly suppressed expression of Alpl (P < 0.01), Bglap (P < 0.001), and Runx2 (P < 0.01), and although parecoxib reduced expression of Alpl (P < 0.001), Fmod (P < 0.001), and Runx2 (P < 0.01), the expression of Bglap was increased (P < 0.01). Microscopic analysis showed a reduction in cellular outgrowth in LPS-stimulated cultures following exposure to ibuprofen and parecoxib. Nonselective COX inhibition and the specific inhibition of COX-2 led to region-specific reductions in markers of calcification and cell viability. We suggest further in vitro and in vivo studies examining the biologic and biomechanical effects of selective and nonselective COX inhibition. |
| format | Article |
| id | doaj-art-b1855f624f674267a4ae5a183d094966 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-b1855f624f674267a4ae5a183d0949662025-02-03T01:32:13ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/926369926369The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture ModelTim Schwarting0Sebastian Pretzsch1Florian Debus2Steffen Ruchholtz3Philipp Lechler4Department of Trauma, Hand and Reconstructive Surgery, University Hospital Marburg, 35043 Marburg, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, University Hospital Marburg, 35043 Marburg, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, University Hospital Marburg, 35043 Marburg, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, University Hospital Marburg, 35043 Marburg, GermanyDepartment of Trauma, Hand and Reconstructive Surgery, University Hospital Marburg, 35043 Marburg, GermanyThe effects of cyclooxygenase (COX) inhibition following the reconstruction of the anterior cruciate ligament remain unclear. We examined the effects of selective COX-2 and nonselective COX inhibition on bone-tendon integration in an in vitro model. We measured the dose-dependent effects of ibuprofen and parecoxib on the viability of lipopolysaccharide- (LPS-) stimulated and unstimulated mouse MC3T3-E1 and 3T3 cells, the influence on gene expression at the osteoblast, interface, and fibroblast regions measured by quantitative PCR, and cellular outgrowth assessed on histological sections. Ibuprofen led to a dose-dependent suppression of MC3T3 cell viability, while parecoxib reduced the viability of 3T3 cultures. Exposure to ibuprofen significantly suppressed expression of Alpl (P < 0.01), Bglap (P < 0.001), and Runx2 (P < 0.01), and although parecoxib reduced expression of Alpl (P < 0.001), Fmod (P < 0.001), and Runx2 (P < 0.01), the expression of Bglap was increased (P < 0.01). Microscopic analysis showed a reduction in cellular outgrowth in LPS-stimulated cultures following exposure to ibuprofen and parecoxib. Nonselective COX inhibition and the specific inhibition of COX-2 led to region-specific reductions in markers of calcification and cell viability. We suggest further in vitro and in vivo studies examining the biologic and biomechanical effects of selective and nonselective COX inhibition.http://dx.doi.org/10.1155/2015/926369 |
| spellingShingle | Tim Schwarting Sebastian Pretzsch Florian Debus Steffen Ruchholtz Philipp Lechler The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model Mediators of Inflammation |
| title | The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model |
| title_full | The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model |
| title_fullStr | The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model |
| title_full_unstemmed | The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model |
| title_short | The Effect of Cyclooxygenase Inhibition on Tendon-Bone Healing in an In Vitro Coculture Model |
| title_sort | effect of cyclooxygenase inhibition on tendon bone healing in an in vitro coculture model |
| url | http://dx.doi.org/10.1155/2015/926369 |
| work_keys_str_mv | AT timschwarting theeffectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT sebastianpretzsch theeffectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT floriandebus theeffectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT steffenruchholtz theeffectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT philipplechler theeffectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT timschwarting effectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT sebastianpretzsch effectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT floriandebus effectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT steffenruchholtz effectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel AT philipplechler effectofcyclooxygenaseinhibitionontendonbonehealinginaninvitrococulturemodel |