Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic Disorders
Gastric and duodenal biopsies from 90 patients with various acid peptic disorders - reflux esophagitis (n=24), gastric ulcer (n=13), duodenal ulcer (n=47) and nonulcer dyspepsia (n=6) - were examined. Seven patients with minimal dyspeptic symptoms and an endoscopically and histologically normal stom...
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| Format: | Article |
| Language: | English |
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Wiley
1996-01-01
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| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/1996/245908 |
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| author | WR Yacoub ABR Thomson P Hooper LD Jewell |
| author_facet | WR Yacoub ABR Thomson P Hooper LD Jewell |
| author_sort | WR Yacoub |
| collection | DOAJ |
| description | Gastric and duodenal biopsies from 90 patients with various acid peptic disorders - reflux esophagitis (n=24), gastric ulcer (n=13), duodenal ulcer (n=47) and nonulcer dyspepsia (n=6) - were examined. Seven patients with minimal dyspeptic symptoms and an endoscopically and histologically normal stomach and duodenum served as controls. Immunoperoxidase staining for gastrin-producing G cells, somatostatin-producing D cells and serotonin-producing EC cells was carried out on fundic, antral and duodenal biopsies, and was quantified using a Zeiss MOP Videoplan using the peroxidase-antiperoxidase technique of Sternberger. In the gastric antrum, a G:D:EC cell ratio of approximately 1.6:1:1 was observed. In the duodenum the corresponding ratio was 1:1:2.4. No significant differences were observed within any of the major diagnostic categories. Patient age, sex, duration of symptoms, smoking habits, alcohol consumption and nonsteroidal anti-inflammatory drug use had no effect on endocrine cell densities. Reduced G cell density in the descending duodenum was observed in the presence of mild duodenitis in four patients. In four patients with evidence of antral intestinal metaplastic changes, a significant increase in duodenal G cell densities was found. These results suggest that a change in the number of G, D or EC cells does not play a primary role in the pathophysiology of acid peptic disorders in the majority of patients. |
| format | Article |
| id | doaj-art-b139f1a775884b22a9f0f36b42ab852d |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-b139f1a775884b22a9f0f36b42ab852d2025-02-03T07:25:37ZengWileyCanadian Journal of Gastroenterology0835-79001996-01-0110639540010.1155/1996/245908Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic DisordersWR Yacoub0ABR Thomson1P Hooper2LD Jewell3Departments of Pathology and Medicine (Division of Gastroenterology) and the Department of Statistics and Applied Probabilities, University of Alberta, Edmonton, Alberta, CanadaDepartments of Pathology and Medicine (Division of Gastroenterology) and the Department of Statistics and Applied Probabilities, University of Alberta, Edmonton, Alberta, CanadaDepartments of Pathology and Medicine (Division of Gastroenterology) and the Department of Statistics and Applied Probabilities, University of Alberta, Edmonton, Alberta, CanadaDepartments of Pathology and Medicine (Division of Gastroenterology) and the Department of Statistics and Applied Probabilities, University of Alberta, Edmonton, Alberta, CanadaGastric and duodenal biopsies from 90 patients with various acid peptic disorders - reflux esophagitis (n=24), gastric ulcer (n=13), duodenal ulcer (n=47) and nonulcer dyspepsia (n=6) - were examined. Seven patients with minimal dyspeptic symptoms and an endoscopically and histologically normal stomach and duodenum served as controls. Immunoperoxidase staining for gastrin-producing G cells, somatostatin-producing D cells and serotonin-producing EC cells was carried out on fundic, antral and duodenal biopsies, and was quantified using a Zeiss MOP Videoplan using the peroxidase-antiperoxidase technique of Sternberger. In the gastric antrum, a G:D:EC cell ratio of approximately 1.6:1:1 was observed. In the duodenum the corresponding ratio was 1:1:2.4. No significant differences were observed within any of the major diagnostic categories. Patient age, sex, duration of symptoms, smoking habits, alcohol consumption and nonsteroidal anti-inflammatory drug use had no effect on endocrine cell densities. Reduced G cell density in the descending duodenum was observed in the presence of mild duodenitis in four patients. In four patients with evidence of antral intestinal metaplastic changes, a significant increase in duodenal G cell densities was found. These results suggest that a change in the number of G, D or EC cells does not play a primary role in the pathophysiology of acid peptic disorders in the majority of patients.http://dx.doi.org/10.1155/1996/245908 |
| spellingShingle | WR Yacoub ABR Thomson P Hooper LD Jewell Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic Disorders Canadian Journal of Gastroenterology |
| title | Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic Disorders |
| title_full | Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic Disorders |
| title_fullStr | Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic Disorders |
| title_full_unstemmed | Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic Disorders |
| title_short | Immunocytochemical and Morphometric Studies of Gastrin-, Somatostatin- and Serotonin-Producing Cells in the Stomach and Duodenum of Patients with Acid Peptic Disorders |
| title_sort | immunocytochemical and morphometric studies of gastrin somatostatin and serotonin producing cells in the stomach and duodenum of patients with acid peptic disorders |
| url | http://dx.doi.org/10.1155/1996/245908 |
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