Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway
In previous studies, Lycium barbarum polysaccharides (LBP), a traditional Chinese medicine, can promote immature dendritic cells (DCs) to mature. However, the molecular mechanisms by which LBP works are not yet elucidated. Here, we found that LBP can induce DCs maturation, which is mainly characteri...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/1751793 |
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| author | Xiangguo Duan Yaru Lan Xiaoyu Zhang Shaozhang Hou Jian Chen Bin Ma Yuhan Xia Chunxia Su |
| author_facet | Xiangguo Duan Yaru Lan Xiaoyu Zhang Shaozhang Hou Jian Chen Bin Ma Yuhan Xia Chunxia Su |
| author_sort | Xiangguo Duan |
| collection | DOAJ |
| description | In previous studies, Lycium barbarum polysaccharides (LBP), a traditional Chinese medicine, can promote immature dendritic cells (DCs) to mature. However, the molecular mechanisms by which LBP works are not yet elucidated. Here, we found that LBP can induce DCs maturation, which is mainly characterized by the upregulation of MHCII and costimulatory molecules (CD80, CD86), and increase the production of IL-6 and IL-4. Furthermore, we found that LBP could increase the mRNA and protein expression of TLR4, p38, Erk1/2, JNK, and Blimp1 signal molecules. More interestingly, after blocking by Toll-like receptor 4 inhibitor, Resatorvid (TAK 242), the mRNA and protein expression of TLR4, Erk1/2, and Blimp1 was significantly decreased while the expression of p38 and JNK has not changed. Then, we found that after blocking by p38 inhibitor (SB203580), Erk inhibitor (PD98059), and JNK inhibitor (SP603580) separately, Blimp1 protein expression was significantly reduced; after downregulating Blimp1 by Blimp1-siRNA, the production of IL-6 was reduced. In conclusion, our results indicate that LBP can induce maturation of DCs through the TLR4-Erk1/2-Blimp1 signal pathway instead of the JNK/p38-Blimp1 pathway. Our findings may provide a novel evidence for understanding the molecular mechanisms of LBP on activating murine DCs. |
| format | Article |
| id | doaj-art-b1380969bf694466a0aef311c8e0b601 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-b1380969bf694466a0aef311c8e0b6012025-02-03T06:43:36ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/17517931751793Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling PathwayXiangguo Duan0Yaru Lan1Xiaoyu Zhang2Shaozhang Hou3Jian Chen4Bin Ma5Yuhan Xia6Chunxia Su7Department of Laboratory Medicine, College of Clinical Medicine, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, ChinaSchool of Basic Medical Sciences, Ningxia Medical University, Yinchuan 750004, ChinaNingxia Innovation Team of the Foundation and Clinical Researches of Diabetes and Its Complications, Yinchuan 750004, ChinaGuolong Hospital, Yinchuan 750004, ChinaDepartment of Oncology Surgery, The First People’s Hospital of Yinchuan, Yinchuan 750004, ChinaGeneral Hospital of Ningxia Medical University, Yinchuan 750004, ChinaNingxia Innovation Team of the Foundation and Clinical Researches of Diabetes and Its Complications, Yinchuan 750004, ChinaIn previous studies, Lycium barbarum polysaccharides (LBP), a traditional Chinese medicine, can promote immature dendritic cells (DCs) to mature. However, the molecular mechanisms by which LBP works are not yet elucidated. Here, we found that LBP can induce DCs maturation, which is mainly characterized by the upregulation of MHCII and costimulatory molecules (CD80, CD86), and increase the production of IL-6 and IL-4. Furthermore, we found that LBP could increase the mRNA and protein expression of TLR4, p38, Erk1/2, JNK, and Blimp1 signal molecules. More interestingly, after blocking by Toll-like receptor 4 inhibitor, Resatorvid (TAK 242), the mRNA and protein expression of TLR4, Erk1/2, and Blimp1 was significantly decreased while the expression of p38 and JNK has not changed. Then, we found that after blocking by p38 inhibitor (SB203580), Erk inhibitor (PD98059), and JNK inhibitor (SP603580) separately, Blimp1 protein expression was significantly reduced; after downregulating Blimp1 by Blimp1-siRNA, the production of IL-6 was reduced. In conclusion, our results indicate that LBP can induce maturation of DCs through the TLR4-Erk1/2-Blimp1 signal pathway instead of the JNK/p38-Blimp1 pathway. Our findings may provide a novel evidence for understanding the molecular mechanisms of LBP on activating murine DCs.http://dx.doi.org/10.1155/2020/1751793 |
| spellingShingle | Xiangguo Duan Yaru Lan Xiaoyu Zhang Shaozhang Hou Jian Chen Bin Ma Yuhan Xia Chunxia Su Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway Journal of Immunology Research |
| title | Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway |
| title_full | Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway |
| title_fullStr | Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway |
| title_full_unstemmed | Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway |
| title_short | Lycium barbarum Polysaccharides Promote Maturity of Murine Dendritic Cells through Toll-Like Receptor 4-Erk1/2-Blimp1 Signaling Pathway |
| title_sort | lycium barbarum polysaccharides promote maturity of murine dendritic cells through toll like receptor 4 erk1 2 blimp1 signaling pathway |
| url | http://dx.doi.org/10.1155/2020/1751793 |
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