A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

A systematic screening assay identifies efficient small guide RNAs for CRISPR activation

CRISPR-mediated gene activation (CRISPRa) encompasses a growing field of biotechnological approaches with exciting implications for gene therapy. However, there is a lack of experimental validation tools for selecting efficient sgRNAs for downstream applications. Here, we present a screening assay c...

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Bibliographic Details
Main Authors: Elin Arvidsson, Diana Duarte Lobo, Ermelinda Sabarese, Fabio Duarte, Rui Jorge Nobre, Luis Quintino, Cecilia Lundberg
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
CRISPRa
gene activation
gene therapy
Tfeb
Adam17
Sirt1
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2025.1336313/full
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