Impact of Nanoparticles as an Air Pollutant on Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor in Asthma

Background: The tricellular tight junction protein angulin-1/lipolysis-stimulated lipoprotein receptor (LSR) is linked to numerous signal transduction pathways that govern gene expression, epithelial cell function, and morphogenesis. The effect of titanium dioxide (TiO<sub>2</sub>) on LS...

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Main Authors: DaYeon Hwang, Min-Hyeok An, Pureun-Haneul Lee, Jung-Hyun Kim, Yunha Nam, Shinhee Park, Ae-Rin Baek, An-Soo Jang
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Atmosphere
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Online Access:https://www.mdpi.com/2073-4433/15/12/1532
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author DaYeon Hwang
Min-Hyeok An
Pureun-Haneul Lee
Jung-Hyun Kim
Yunha Nam
Shinhee Park
Ae-Rin Baek
An-Soo Jang
author_facet DaYeon Hwang
Min-Hyeok An
Pureun-Haneul Lee
Jung-Hyun Kim
Yunha Nam
Shinhee Park
Ae-Rin Baek
An-Soo Jang
author_sort DaYeon Hwang
collection DOAJ
description Background: The tricellular tight junction protein angulin-1/lipolysis-stimulated lipoprotein receptor (LSR) is linked to numerous signal transduction pathways that govern gene expression, epithelial cell function, and morphogenesis. The effect of titanium dioxide (TiO<sub>2</sub>) on LSR and asthma remains unknown. The objective of the present study was to evaluate the impact of TiO<sub>2</sub> on LSR expression in asthma. Methods: A TiO<sub>2</sub>-induced animal model of asthma was established using BALB/c mice and cell lines using normal human bronchial epithelial (NHBE) lung cells and we examined LSR, RAGE, and TGFβ expression using this model. Additionally, we analyzed plasma-LSR concentrations and their correlation with clinical variables in asthma patients and control subjects. Results: The LSR concentrations in patients with asthma were lower compared to controls, and were correlated with lung function and inflammatory cell ratio. In NHBE cells treated with <i>Derp1</i>, LSR protein expression was reduced and changed by exposure to TiO<sub>2</sub>, whereas TGFβ expression was increased and changed. In mouse lungs, LSR expression was significantly reduced in OVA mice and changed in OVA/TiO<sub>2</sub> mice. Conclusion: Circulating LSR levels were decreased and correlated with clinical variables in patients with asthma, and they were influenced by TiO<sub>2</sub> exposure in mice, suggesting the potential involvement of LSR in asthma pathogenesis.
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spelling doaj-art-b104fdf3e11d4ece865587293c3dfaf72025-08-20T02:01:06ZengMDPI AGAtmosphere2073-44332024-12-011512153210.3390/atmos15121532Impact of Nanoparticles as an Air Pollutant on Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor in AsthmaDaYeon Hwang0Min-Hyeok An1Pureun-Haneul Lee2Jung-Hyun Kim3Yunha Nam4Shinhee Park5Ae-Rin Baek6An-Soo Jang7Department of Internal Medicine, Soonchunhyang University Bucheon Hospital, 170 Jomaru-ro, Bucheon-si 14584, Republic of KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, 170 Jomaru-ro, Bucheon-si 14584, Republic of KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, 170 Jomaru-ro, Bucheon-si 14584, Republic of KoreaDepartment of Internal Medicine, Korean Armed Forces Capital Hospital, Seongnam 13574, Republic of KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, 170 Jomaru-ro, Bucheon-si 14584, Republic of KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, 170 Jomaru-ro, Bucheon-si 14584, Republic of KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, 170 Jomaru-ro, Bucheon-si 14584, Republic of KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, 170 Jomaru-ro, Bucheon-si 14584, Republic of KoreaBackground: The tricellular tight junction protein angulin-1/lipolysis-stimulated lipoprotein receptor (LSR) is linked to numerous signal transduction pathways that govern gene expression, epithelial cell function, and morphogenesis. The effect of titanium dioxide (TiO<sub>2</sub>) on LSR and asthma remains unknown. The objective of the present study was to evaluate the impact of TiO<sub>2</sub> on LSR expression in asthma. Methods: A TiO<sub>2</sub>-induced animal model of asthma was established using BALB/c mice and cell lines using normal human bronchial epithelial (NHBE) lung cells and we examined LSR, RAGE, and TGFβ expression using this model. Additionally, we analyzed plasma-LSR concentrations and their correlation with clinical variables in asthma patients and control subjects. Results: The LSR concentrations in patients with asthma were lower compared to controls, and were correlated with lung function and inflammatory cell ratio. In NHBE cells treated with <i>Derp1</i>, LSR protein expression was reduced and changed by exposure to TiO<sub>2</sub>, whereas TGFβ expression was increased and changed. In mouse lungs, LSR expression was significantly reduced in OVA mice and changed in OVA/TiO<sub>2</sub> mice. Conclusion: Circulating LSR levels were decreased and correlated with clinical variables in patients with asthma, and they were influenced by TiO<sub>2</sub> exposure in mice, suggesting the potential involvement of LSR in asthma pathogenesis.https://www.mdpi.com/2073-4433/15/12/1532cell barriersLSRbronchial asthmaair pollutantstitanium dioxide
spellingShingle DaYeon Hwang
Min-Hyeok An
Pureun-Haneul Lee
Jung-Hyun Kim
Yunha Nam
Shinhee Park
Ae-Rin Baek
An-Soo Jang
Impact of Nanoparticles as an Air Pollutant on Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor in Asthma
Atmosphere
cell barriers
LSR
bronchial asthma
air pollutants
titanium dioxide
title Impact of Nanoparticles as an Air Pollutant on Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor in Asthma
title_full Impact of Nanoparticles as an Air Pollutant on Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor in Asthma
title_fullStr Impact of Nanoparticles as an Air Pollutant on Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor in Asthma
title_full_unstemmed Impact of Nanoparticles as an Air Pollutant on Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor in Asthma
title_short Impact of Nanoparticles as an Air Pollutant on Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor in Asthma
title_sort impact of nanoparticles as an air pollutant on angulin 1 lipolysis stimulated lipoprotein receptor in asthma
topic cell barriers
LSR
bronchial asthma
air pollutants
titanium dioxide
url https://www.mdpi.com/2073-4433/15/12/1532
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