Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents
Rapid, eco-friendly, and cost-effective one-pot synthesis of copper nanoparticles is reported here using medicinal plants like Gnidia glauca and Plumbago zeylanica. Aqueous extracts of flower, leaf, and stem of G. glauca and leaves of P. zeylanica were prepared which could effectively reduce Cu2+ io...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2019/9080279 |
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| author | Dhiraj A. Jamdade Dishantsingh Rajpali Komal A. Joshi Rohini Kitture Anuja S. Kulkarni Vaishali S. Shinde Jayesh Bellare Kaushik R. Babiya Sougata Ghosh |
| author_facet | Dhiraj A. Jamdade Dishantsingh Rajpali Komal A. Joshi Rohini Kitture Anuja S. Kulkarni Vaishali S. Shinde Jayesh Bellare Kaushik R. Babiya Sougata Ghosh |
| author_sort | Dhiraj A. Jamdade |
| collection | DOAJ |
| description | Rapid, eco-friendly, and cost-effective one-pot synthesis of copper nanoparticles is reported here using medicinal plants like Gnidia glauca and Plumbago zeylanica. Aqueous extracts of flower, leaf, and stem of G. glauca and leaves of P. zeylanica were prepared which could effectively reduce Cu2+ ions to CuNPs within 5 h at 100°C which were further characterized using UV-visible spectroscopy, field emission scanning electron microscopy, high-resolution transmission electron microscopy, energy dispersive spectroscopy, dynamic light scattering, X-ray diffraction, and Fourier-transform infrared spectroscopy. Further, the CuNPs were checked for antidiabetic activity using porcine pancreatic α-amylase and α-glucosidase inhibition followed by evaluation of mechanism using circular dichroism spectroscopy. CuNPs were found to be predominantly spherical in nature with a diameter ranging from 1 to 5 nm. The phenolics and flavonoids in the extracts might play a critical role in the synthesis and stabilization process. Significant change in the peak at ∼1095 cm−1 corresponding to C-O-C bond in ether was observed. CuNPs could inhibit porcine pancreatic α-amylase up to 30% to 50%, while they exhibited a more significant inhibition of α-glucosidase from 70% to 88%. The mechanism of enzyme inhibition was attributed due to the conformational change owing to drastic alteration of secondary structure by CuNPs. This is the first study of its kind that provides a strong scientific rationale that phytogenic CuNPs synthesized using G. glauca and P. zeylanica can be considered to develop candidate antidiabetic nanomedicine. |
| format | Article |
| id | doaj-art-b0dc6a2e009f4dbeb1f14fc801a70d7d |
| institution | Kabale University |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-b0dc6a2e009f4dbeb1f14fc801a70d7d2025-02-03T05:59:35ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422019-01-01201910.1155/2019/90802799080279Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic AgentsDhiraj A. Jamdade0Dishantsingh Rajpali1Komal A. Joshi2Rohini Kitture3Anuja S. Kulkarni4Vaishali S. Shinde5Jayesh Bellare6Kaushik R. Babiya7Sougata Ghosh8Department of Microbiology, Modern College of Arts, Science and Commerce, Ganeshkhind, Pune 411016, IndiaDepartment of Microbiology, Modern College of Arts, Science and Commerce, Ganeshkhind, Pune 411016, IndiaInstitute of Bioinformatics and Biotechnology, Savitribai Phule Pune University, Pune 411007, IndiaDepartment of Applied Physics, Defense Institute of Advanced Technology, Girinagar, Pune 411025, IndiaDepartment of Chemistry, Savitribai Phule Pune University, Pune-411007, IndiaDepartment of Chemistry, Savitribai Phule Pune University, Pune-411007, IndiaDepartment of Chemical Engineering, Indian Institute of Technology, Bombay, Powai, Mumbai 400076, IndiaDepartment of Microbiology, School of Science, RK University, Kasturbadham, Rajkot 360020, IndiaDepartment of Microbiology, School of Science, RK University, Kasturbadham, Rajkot 360020, IndiaRapid, eco-friendly, and cost-effective one-pot synthesis of copper nanoparticles is reported here using medicinal plants like Gnidia glauca and Plumbago zeylanica. Aqueous extracts of flower, leaf, and stem of G. glauca and leaves of P. zeylanica were prepared which could effectively reduce Cu2+ ions to CuNPs within 5 h at 100°C which were further characterized using UV-visible spectroscopy, field emission scanning electron microscopy, high-resolution transmission electron microscopy, energy dispersive spectroscopy, dynamic light scattering, X-ray diffraction, and Fourier-transform infrared spectroscopy. Further, the CuNPs were checked for antidiabetic activity using porcine pancreatic α-amylase and α-glucosidase inhibition followed by evaluation of mechanism using circular dichroism spectroscopy. CuNPs were found to be predominantly spherical in nature with a diameter ranging from 1 to 5 nm. The phenolics and flavonoids in the extracts might play a critical role in the synthesis and stabilization process. Significant change in the peak at ∼1095 cm−1 corresponding to C-O-C bond in ether was observed. CuNPs could inhibit porcine pancreatic α-amylase up to 30% to 50%, while they exhibited a more significant inhibition of α-glucosidase from 70% to 88%. The mechanism of enzyme inhibition was attributed due to the conformational change owing to drastic alteration of secondary structure by CuNPs. This is the first study of its kind that provides a strong scientific rationale that phytogenic CuNPs synthesized using G. glauca and P. zeylanica can be considered to develop candidate antidiabetic nanomedicine.http://dx.doi.org/10.1155/2019/9080279 |
| spellingShingle | Dhiraj A. Jamdade Dishantsingh Rajpali Komal A. Joshi Rohini Kitture Anuja S. Kulkarni Vaishali S. Shinde Jayesh Bellare Kaushik R. Babiya Sougata Ghosh Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents Advances in Pharmacological Sciences |
| title | Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents |
| title_full | Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents |
| title_fullStr | Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents |
| title_full_unstemmed | Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents |
| title_short | Gnidia glauca- and Plumbago zeylanica-Mediated Synthesis of Novel Copper Nanoparticles as Promising Antidiabetic Agents |
| title_sort | gnidia glauca and plumbago zeylanica mediated synthesis of novel copper nanoparticles as promising antidiabetic agents |
| url | http://dx.doi.org/10.1155/2019/9080279 |
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