Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release
Abstract Inflammatory bowel disease (IBD) is characterized by compromised intestinal barrier function and a lack of effective treatments. Probiotics have shown promise in managing IBD due to their ability to modulate the gut microbiota, enhance intestinal barrier function, and exert anti-inflammator...
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BMC
2025-03-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-025-03280-7 |
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| author | Yuanyuan Wu Xinyue Huang Qianbei Li Chaoqun Yang Xixin Huang Hualongyue Du Bo Situ Lei Zheng Zihao Ou |
| author_facet | Yuanyuan Wu Xinyue Huang Qianbei Li Chaoqun Yang Xixin Huang Hualongyue Du Bo Situ Lei Zheng Zihao Ou |
| author_sort | Yuanyuan Wu |
| collection | DOAJ |
| description | Abstract Inflammatory bowel disease (IBD) is characterized by compromised intestinal barrier function and a lack of effective treatments. Probiotics have shown promise in managing IBD due to their ability to modulate the gut microbiota, enhance intestinal barrier function, and exert anti-inflammatory effects. However, the specific mechanisms through which probiotics exert these therapeutic effects in IBD treatment remain poorly understood. Our research revealed a significant reduction of Lactiplantibacillus plantarum (L. plantarum) in the gut microbiota of IBD patients. L. plantarum is a well-known probiotic strain in the list of edible probiotics, recognized for its beneficial effects on gut health, including its ability to strengthen the intestinal barrier and reduce inflammation. We demonstrated that supplementation with L. plantarum could alleviate IBD symptoms in mice, primarily by inhibiting apoptosis in intestinal epithelial cells through L. plantarum’s bacterial extracellular vesicles (L. plant-EVs). This protective effect is dependent on the efficient uptake of L. plant-EVs by intestinal cells. Intriguingly, watermelon enhances L. plantarum colonization and L. plant-EVs release, further promoting intestinal barrier repair. Our findings contribute to the understanding of L. plant-EVs in the probiotic-based therapeutic approach for IBD, as they are promising candidates for nanoparticle-based therapeutic methods that are enhanced by natural diets such as watermelon. This study thereby offers a potential breakthrough in the management and treatment of IBD. Graphical Abstract |
| format | Article |
| id | doaj-art-b0c52edfc42e4e7ea307fcc38e1b0d77 |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj-art-b0c52edfc42e4e7ea307fcc38e1b0d772025-08-20T02:41:33ZengBMCJournal of Nanobiotechnology1477-31552025-03-0123111510.1186/s12951-025-03280-7Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles releaseYuanyuan Wu0Xinyue Huang1Qianbei Li2Chaoqun Yang3Xixin Huang4Hualongyue Du5Bo Situ6Lei Zheng7Zihao Ou8Department of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityDepartment of Laboratory Medicine, Nanfang Hospital, Southern Medical UniversityAbstract Inflammatory bowel disease (IBD) is characterized by compromised intestinal barrier function and a lack of effective treatments. Probiotics have shown promise in managing IBD due to their ability to modulate the gut microbiota, enhance intestinal barrier function, and exert anti-inflammatory effects. However, the specific mechanisms through which probiotics exert these therapeutic effects in IBD treatment remain poorly understood. Our research revealed a significant reduction of Lactiplantibacillus plantarum (L. plantarum) in the gut microbiota of IBD patients. L. plantarum is a well-known probiotic strain in the list of edible probiotics, recognized for its beneficial effects on gut health, including its ability to strengthen the intestinal barrier and reduce inflammation. We demonstrated that supplementation with L. plantarum could alleviate IBD symptoms in mice, primarily by inhibiting apoptosis in intestinal epithelial cells through L. plantarum’s bacterial extracellular vesicles (L. plant-EVs). This protective effect is dependent on the efficient uptake of L. plant-EVs by intestinal cells. Intriguingly, watermelon enhances L. plantarum colonization and L. plant-EVs release, further promoting intestinal barrier repair. Our findings contribute to the understanding of L. plant-EVs in the probiotic-based therapeutic approach for IBD, as they are promising candidates for nanoparticle-based therapeutic methods that are enhanced by natural diets such as watermelon. This study thereby offers a potential breakthrough in the management and treatment of IBD. Graphical Abstracthttps://doi.org/10.1186/s12951-025-03280-7Bacterial extracellular vesiclesLactiplantibacillus plantarumInflammatory bowel diseaseIntestinal barrierWatermelon |
| spellingShingle | Yuanyuan Wu Xinyue Huang Qianbei Li Chaoqun Yang Xixin Huang Hualongyue Du Bo Situ Lei Zheng Zihao Ou Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release Journal of Nanobiotechnology Bacterial extracellular vesicles Lactiplantibacillus plantarum Inflammatory bowel disease Intestinal barrier Watermelon |
| title | Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release |
| title_full | Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release |
| title_fullStr | Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release |
| title_full_unstemmed | Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release |
| title_short | Reducing severity of inflammatory bowel disease through colonization of Lactiplantibacillus plantarum and its extracellular vesicles release |
| title_sort | reducing severity of inflammatory bowel disease through colonization of lactiplantibacillus plantarum and its extracellular vesicles release |
| topic | Bacterial extracellular vesicles Lactiplantibacillus plantarum Inflammatory bowel disease Intestinal barrier Watermelon |
| url | https://doi.org/10.1186/s12951-025-03280-7 |
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