Peel of pumpkin (Cucurbita pepo) diets boost growth, digestive-absorptive functions, immune-antioxidant indices, and regulate immunomodulatory genes and mTOR/MAPK-P38/apoptosis signaling pathway in Oreochromis niloticus
Much attention has been focused on the potential application of industrial by-products as feed supplements in fish diets. The current study examines the effects of feeding pumpkin (Cucurbita pepo) peel powder (CPP) on growth, digestive processes, immune-antioxidant parameters, and gene expression th...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006367 |
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| author | Rowida E. Ibrahim Gehad E. Elshopakey Elsayed M. Younis Abdelwahab A. Abdelwarith Asmaa Yaseen Tarek Khamis Simon J. Davies Afaf N. Abdel Rahman |
| author_facet | Rowida E. Ibrahim Gehad E. Elshopakey Elsayed M. Younis Abdelwahab A. Abdelwarith Asmaa Yaseen Tarek Khamis Simon J. Davies Afaf N. Abdel Rahman |
| author_sort | Rowida E. Ibrahim |
| collection | DOAJ |
| description | Much attention has been focused on the potential application of industrial by-products as feed supplements in fish diets. The current study examines the effects of feeding pumpkin (Cucurbita pepo) peel powder (CPP) on growth, digestive processes, immune-antioxidant parameters, and gene expression that regulates these processes in Nile tilapia (Oreochromis niloticus). Additionally, fish resistance to Aeromonas veronii was assessed. Fish (n = 200; 34.93 ± 0.11 g) were split equally into four groups and kept for 60 days. Fish were given diets supplemented with different CPP levels: 0 (CPP0 was the control diet), 5 (CPP5), 10 (CPP10), and 15 (CPP15) g CPP/kg diet. The findings showed that CPP meals significantly increased (P˂0.05) growth, intestinal morphometrics, and digestive enzyme activity. The optimum dietary level of CPP was 12 g/kg diet according to the broken line regression analysis based on the data of weight gain and feed conversion ratio. CPP diets up-regulated (P˂0.05) the intestinal and muscular gene expression (trypsin, solute carrier family 6, member 18, fatty acid-binding proteins-2, cluster of differentiation 36, insulin growth factor-1, carnitine palmitoyl-transferase-1alpha, pyruvate kinase, and glucokinase). CPP diets improved the immune-antioxidant indices (P˂0.001) (lysozyme, complement 3, myeloperoxidase, superoxide dismutase, reduced glutathione content, and total antioxidant capacity). Moreover, CPP diets increased (P˂0.05) the splenic expression of heme oxygenase-1, interleukins (IL-6 and IL8), tumor necrosis factor-alpha, and nuclear factor erythroid-related factor 2. CPP diets caused down-regulation (P˂0.05) of the splenic expression of the tumor suppressor gene (P53), mitogen-activated protein kinase (P38), caspase-9, and mechanistic target of rapamycin. During the A. veronii challenge, the fish survival percentage increased with CPP diets. Noteworthy, CPP diets could improve Nile tilapia immunity, growth, and disease resistance. Moreover, the best dietary level of CPP was 12 g/kg diet for sustainable Nile tilapia aquaculture. |
| format | Article |
| id | doaj-art-b0bfd3ce58f04619925ae4d50c69dbc5 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj-art-b0bfd3ce58f04619925ae4d50c69dbc52025-02-06T05:12:06ZengElsevierAquaculture Reports2352-51342025-03-0140102548Peel of pumpkin (Cucurbita pepo) diets boost growth, digestive-absorptive functions, immune-antioxidant indices, and regulate immunomodulatory genes and mTOR/MAPK-P38/apoptosis signaling pathway in Oreochromis niloticusRowida E. Ibrahim0Gehad E. Elshopakey1Elsayed M. Younis2Abdelwahab A. Abdelwarith3Asmaa Yaseen4Tarek Khamis5Simon J. Davies6Afaf N. Abdel Rahman7Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt; Corresponding authors.Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, PO Box 35516, Mansoura, Dakahlia, Egypt; Department of Veterinary Diseases, Faculty of Veterinary Medicine, Delta University for Science and Technology, Gamasa 35712, EgyptDepartment of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Zoology, College of Science, King Saud University, PO Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Nutrition and Clinical Nutrition, Faculty of Veterinary Medicine, University of Sadat City, PO Box 32897, Sadat City, Menofia, EgyptDepartment of Pharmacology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptAquaculture Nutrition Research Unit ANRU, Carna Research Station, Ryan Institute, College of Science and Engineering, University of Galway, Galway H91V8Y1, IrelandDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, PO Box 44511, Zagazig, Sharkia, Egypt; Corresponding authors.Much attention has been focused on the potential application of industrial by-products as feed supplements in fish diets. The current study examines the effects of feeding pumpkin (Cucurbita pepo) peel powder (CPP) on growth, digestive processes, immune-antioxidant parameters, and gene expression that regulates these processes in Nile tilapia (Oreochromis niloticus). Additionally, fish resistance to Aeromonas veronii was assessed. Fish (n = 200; 34.93 ± 0.11 g) were split equally into four groups and kept for 60 days. Fish were given diets supplemented with different CPP levels: 0 (CPP0 was the control diet), 5 (CPP5), 10 (CPP10), and 15 (CPP15) g CPP/kg diet. The findings showed that CPP meals significantly increased (P˂0.05) growth, intestinal morphometrics, and digestive enzyme activity. The optimum dietary level of CPP was 12 g/kg diet according to the broken line regression analysis based on the data of weight gain and feed conversion ratio. CPP diets up-regulated (P˂0.05) the intestinal and muscular gene expression (trypsin, solute carrier family 6, member 18, fatty acid-binding proteins-2, cluster of differentiation 36, insulin growth factor-1, carnitine palmitoyl-transferase-1alpha, pyruvate kinase, and glucokinase). CPP diets improved the immune-antioxidant indices (P˂0.001) (lysozyme, complement 3, myeloperoxidase, superoxide dismutase, reduced glutathione content, and total antioxidant capacity). Moreover, CPP diets increased (P˂0.05) the splenic expression of heme oxygenase-1, interleukins (IL-6 and IL8), tumor necrosis factor-alpha, and nuclear factor erythroid-related factor 2. CPP diets caused down-regulation (P˂0.05) of the splenic expression of the tumor suppressor gene (P53), mitogen-activated protein kinase (P38), caspase-9, and mechanistic target of rapamycin. During the A. veronii challenge, the fish survival percentage increased with CPP diets. Noteworthy, CPP diets could improve Nile tilapia immunity, growth, and disease resistance. Moreover, the best dietary level of CPP was 12 g/kg diet for sustainable Nile tilapia aquaculture.http://www.sciencedirect.com/science/article/pii/S2352513424006367Aeromonas veroniiDigestive enzymesFeed supplementImmunityNile tilapiaPumpkins |
| spellingShingle | Rowida E. Ibrahim Gehad E. Elshopakey Elsayed M. Younis Abdelwahab A. Abdelwarith Asmaa Yaseen Tarek Khamis Simon J. Davies Afaf N. Abdel Rahman Peel of pumpkin (Cucurbita pepo) diets boost growth, digestive-absorptive functions, immune-antioxidant indices, and regulate immunomodulatory genes and mTOR/MAPK-P38/apoptosis signaling pathway in Oreochromis niloticus Aquaculture Reports Aeromonas veronii Digestive enzymes Feed supplement Immunity Nile tilapia Pumpkins |
| title | Peel of pumpkin (Cucurbita pepo) diets boost growth, digestive-absorptive functions, immune-antioxidant indices, and regulate immunomodulatory genes and mTOR/MAPK-P38/apoptosis signaling pathway in Oreochromis niloticus |
| title_full | Peel of pumpkin (Cucurbita pepo) diets boost growth, digestive-absorptive functions, immune-antioxidant indices, and regulate immunomodulatory genes and mTOR/MAPK-P38/apoptosis signaling pathway in Oreochromis niloticus |
| title_fullStr | Peel of pumpkin (Cucurbita pepo) diets boost growth, digestive-absorptive functions, immune-antioxidant indices, and regulate immunomodulatory genes and mTOR/MAPK-P38/apoptosis signaling pathway in Oreochromis niloticus |
| title_full_unstemmed | Peel of pumpkin (Cucurbita pepo) diets boost growth, digestive-absorptive functions, immune-antioxidant indices, and regulate immunomodulatory genes and mTOR/MAPK-P38/apoptosis signaling pathway in Oreochromis niloticus |
| title_short | Peel of pumpkin (Cucurbita pepo) diets boost growth, digestive-absorptive functions, immune-antioxidant indices, and regulate immunomodulatory genes and mTOR/MAPK-P38/apoptosis signaling pathway in Oreochromis niloticus |
| title_sort | peel of pumpkin cucurbita pepo diets boost growth digestive absorptive functions immune antioxidant indices and regulate immunomodulatory genes and mtor mapk p38 apoptosis signaling pathway in oreochromis niloticus |
| topic | Aeromonas veronii Digestive enzymes Feed supplement Immunity Nile tilapia Pumpkins |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006367 |
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